@article{schmitz_schwartz_catignani_1994, title={RESOLUTION AND QUANTITATION OF THE PREDOMINANT GEOMETRIC BETA-CAROTENE ISOMERS PRESENT IN HUMAN SERUM USING NORMAL-PHASE HPLC}, volume={42}, ISSN={["0021-8561"]}, DOI={10.1021/jf00048a019}, abstractNote={An isocratic high-performance liquid chromatography (HPLC) method completely resolving all-trans-, 9-cis-, and 13-cis-β-carotenes in human serum was developed. The chromatographic separation was achieved on a calcium hydroxide stationary phase following sample extraction and fractionation on fully activated neutral alumina. A combination of p-methylanisole and acetone was used to modify the hexane (1:1:98) mobile phase, which improved the tailing of later eluting peaks generally associated with chromatographic separations on calcium hydroxide. Sample fractionation on alumina was used to remove components present in serum that interfered with the HPLC separation of β-carotene isomers}, number={12}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={SCHMITZ, HH and SCHWARTZ, SJ and CATIGNANI, GL}, year={1994}, month={Dec}, pages={2746–2750} }