@article{venkatesh_abdel-aal_armstrong_roe_1990, title={Characterization of affinity-purified juvenile hormone esterase from the plasma of the tobacco hornworm, Manduca sexta}, volume={265}, number={35}, journal={Journal of Biological Chemistry}, author={Venkatesh, K. and Abdel-Aal, Y. A. I. and Armstrong, F. B. and Roe, R. M.}, year={1990}, pages={21727} }
 @article{venkatesh_roe_apperson_sonenshine_schriefer_boland_1990, title={METABOLISM OF JUVENILE-HORMONE DURING ADULT DEVELOPMENT OF DERMACENTOR-VARIABILIS (ACARI, IXODIDAE)}, volume={27}, ISSN={["0022-2585"]}, DOI={10.1093/jmedent/27.1.36}, abstractNote={Juvenile hormone (JH)-I and -III were used as model substrates to study the in vitro metabolism of JH in the hemolymph and body homogenates of the American dog tick, Dermacentor variabilis (Say). Ester hydrolysis was the principal pathway of JH metabolism in hemolymph and homogenates. JH also was converted into JH-diol primarily by body homogenates, indicating the presence of JH epoxide hydrolase activity. JH epoxide hydrolase activity, alpha-naphthyl acetate esterase activity, and protein concentration per milligram wet weight were significantly lower (t test, alpha = 0.05) in homogenates of partially fed, virgin and replete, mated females of D. variabilis compared with unfed, virgin females. The decline in these factors was probably because of the influx of water into the tissues caused by the blood meal. In addition, the epoxide hydrolase and alpha-naphthyl acetate esterase activity per milligram tissue protein decreased significantly during this time. Mating of fed females rather than feeding alone caused a significant decline in the tissue JH esterase activity per milligram wet weight but not per milligram protein. The JH esterase activity per milligram protein was significantly higher in partially fed, virgin and replete, mated females compared with unfed females, indicating that feeding may actually increase JH esterase activity on a protein basis. JH-III was metabolized 1.4 times faster than JH-I by the hemolymph of partially fed, virgin females. The inhibitors O,O-diisopropyl phosphorofluoridate and octylthio-1,1,1-trifluoropropan-2-one at 10(-4) M inhibited JH and alpha-naphthyl acetate esterase activity in hemolymph and body homogenate.}, number={1}, journal={JOURNAL OF MEDICAL ENTOMOLOGY}, author={VENKATESH, K and ROE, RM and APPERSON, CS and SONENSHINE, DE and SCHRIEFER, ME and BOLAND, LM}, year={1990}, month={Jan}, pages={36–42} }
 @article{venkatesh_roe_1989, title={THE REGULATION OF THE 1ST PEAK OF HEMOLYMPH JUVENILE-HORMONE ESTERASE-ACTIVITY DURING THE LAST STADIUM OF THE CABBAGE-LOOPER, TRICHOPLUSIA-NI}, volume={35}, ISSN={["0022-1910"]}, DOI={10.1016/0022-1910(89)90142-X}, abstractNote={The role of the head and a juvenoid, epofenonane, in the regulation of the first peak of haemolymph juvenile hormone esterase activity was examined during the fifth (last) stadium of the cabbage looper, Trichoplusia ni. The topical application of epofenonane to unligated day-1 larvae brought about an average 36% increase in the haemolymph juvenile hormone esterase activity. Epofenonane treatment also resulted in a 2-fold increase in the juvenile hormone and α-naphthyl acetate esterase activity of the whole body of unligated larvae suggesting that the juvenoid causes an increase in esterase biosynthesis or activation. Ligation and starvation prevented the appearance of the first peak of haemolymph juvenile hormone esterase activity. Application of epofenonane to newly ligated larvae had no effect whereas it significantly increased the juvenile hormone and α-naphthyl acetate esterase activity of the haemolymph in larvae ligated for 24 h prior to the treatment. In newly starved larvae, epofenonane increased the haemolymph juvenile hormone and α-naphthyl acetate esterase activity above respective controls. In 24 h starved larvae, juvenile hormone esterase activity of the haemolymph increased in response to epofenonane, head extract, or a combination of head extract injection and epofenonane application whereas α-naphthyl acetate esterase activity increased only to head extract or to a combination of head extract injection and epofenonane application. Injection of head extracts of day-1, fifth-instar larvae into the same aged ligated larvae increased both the juvenile hormone and α-naphthyl acetate esterase activity in the haemolymph by about 3-fold 5 h later compared to the respective controls. This increase, however, was not reflected in a similar increase in the whole body esterase levels suggesting that head extract may have caused a release of esterases from tissues into the haemolymph. Injection of head extract from day-1 larvae into the same aged unligated larvae inhibited the normal increase in the haemolymph juvenile hormone esterase activity seen between day 1 and 2 and no inhibition was noted with injections of body extracts or bovine serum albumin. Head extract injection significantly decreased the juvenile hormone esterase activity in the whole body, and haemolymph of unligated and 24 h ligated larvae treated with epofenonane. The head-extract-induced inhibition of haemolymph juvenile hormone esterase activity was dose dependent and the esterase activity in the haemolymph was unaffected when incubated in vitro with head extract for a period of 4 days at 30°C.}, number={7}, journal={JOURNAL OF INSECT PHYSIOLOGY}, author={VENKATESH, K and ROE, RM}, year={1989}, pages={543–551} }