@misc{riggs_perryman_2004, title={Anti-cryptosporidium parvum preparations}, volume={6,682,737}, number={2004 Jan. 27}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Riggs, M. W. and Perryman, L. E.}, year={2004} }
 @misc{perryman_jasmer_riggs_mcguire_2004, title={Neutralization-sensitive epitopes of Cryptosporidium parvum}, volume={6,759,048}, number={2004 July 06}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Perryman, L. E. and Jasmer, D. P. and Riggs, M. W. and McGuire, T. C.}, year={2004} }
 @article{riggs_schaefer_kapil_barley-maloney_perryman_2002, title={Efficacy of monoclonal antibodies against defined antigens for passive immunotherapy of chronic gastrointestinal cryptosporidiosis}, volume={46}, ISSN={["1098-6596"]}, DOI={10.1128/AAC.46.2.275-282.2002}, abstractNote={ABSTRACT}, number={2}, journal={ANTIMICROBIAL AGENTS AND CHEMOTHERAPY}, author={Riggs, MW and Schaefer, DA and Kapil, SJ and Barley-Maloney, L and Perryman, LE}, year={2002}, month={Feb}, pages={275–282} }
 @misc{perryman_jasmer_riggs_mcguire_2001, title={Neutralization-sensitive epitopes of Cryptosporidium parvum}, volume={6,323,020}, number={2001 Nov. 27}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Perryman, L. E. and Jasmer, D. P. and Riggs, M. W. and McGuire, T. C.}, year={2001} }
 @article{riggs_schaefer_kapil_barley-maloney_perryman_mcneil_2001, title={Targeted disruption of CSL ligand-host cell receptor interaction in treatment of Cryptosporidium parvum infection}, number={2001}, journal={Journal of Eukaryotic Microbiology}, author={Riggs, M. W. and Schaefer, D. A. and Kapil, S. J. and Barley-Maloney, L. and Perryman, L. E. and McNeil, M. R.}, year={2001}, pages={44S–46} }
 @misc{riggs_perryman_2000, title={Anti-Cryptosporidium parvum preparations}, volume={6,110,463}, number={2000 Aug. 29}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Riggs, M. W. and Perryman, L. E.}, year={2000} }
 @inbook{wyatt_perryman_2000, title={Detection of mucosally delivered antibody to Cryptosporidium parvum p23 in infected calves}, volume={916}, number={2000}, booktitle={Tropical veterinary diseases: Control and prevention in the context of the new world order}, publisher={New York, NY: New York Academy of Sciences}, author={Wyatt, C. R. and Perryman, L. E.}, editor={J. A. House, K. M. Kocan and Gibbs, E. P. J.Editors}, year={2000}, pages={378–387} }
 @article{wyatt_brackett_mason_savidge_perryman_2000, title={Excretion patterns of mucosally delivered antibodies to p23 in Cryptosporidium parvum infected calves}, volume={76}, ISSN={["0165-2427"]}, DOI={10.1016/S0165-2427(00)00218-X}, abstractNote={Fecal samples obtained at intervals from six calves with acute cryptosporidiosis contained antibodies of multiple isotypes to p23. IgM-, IgA-, and IgG(1)-isotype anti-p23 appeared before IgG(2)-isotype antibodies. All anti-p23 antibodies had declined by 2 months after infection. One calf that failed to shed oocysts following initial exposure developed IgG(1)-isotype anti-p23 antibodies. One calf that died following exposure to Cryptosporidium parvum oocysts lacked detectable anti-p23 antibodies. Re-inoculation with C. parvum resulted in a brief, marked recall response to p23.}, number={3-4}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Wyatt, CR and Brackett, EJ and Mason, PH and Savidge, J and Perryman, LE}, year={2000}, month={Oct}, pages={309–317} }
 @article{perryman_2000, title={Primary immunodeficiencies of horses}, volume={16}, ISSN={["1558-4224"]}, DOI={10.1016/S0749-0739(17)30121-9}, abstractNote={Primary immunodeficiency disorders are genetically determined failures of immune defense that increase susceptibility to infectious agents. This article reviews the salient features of equine primary immunodeficiency disorders, summarizes the molecular mechanisms of each disorder, and updates information that facilitates diagnosis and management of affected horses. The central theme is to encourage clinicians to ask, “I wonder if this horse has an underlying primary immunodeficiency disorder?” when caring for horses suffering from chronic and recurring infections and responding poorly to standard therapy.}, number={1}, journal={VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE}, author={Perryman, LE}, year={2000}, month={Apr}, pages={105-+} }
 @article{riggs_mcneil_perryman_stone_scherman_o'connor_1999, title={Cryptosporidium parvum sporozoite pellicle antigen recognized by a neutralizing monoclonal antibody is a beta-mannosylated glycolipid}, volume={67}, number={3}, journal={Infection and Immunity}, author={Riggs, M. W. and McNeil, M. R. and Perryman, L. E. and Stone, A. L. and Scherman, M. S. and O'Connor, R. M.}, year={1999}, pages={1317–1322} }
 @article{kappmeyer_perryman_hines_baszler_katz_hennager_knowles_1999, title={Detection of equine antibodies to Babesia Caballi by recombinant B-Caballi Rhoptry-associated protein 1 in a competitive-Inhibition enzyme-linked immunosorbent assay}, volume={37}, number={7}, journal={Journal of Clinical Microbiology}, author={Kappmeyer, L. S. and Perryman, L. E. and Hines, S. A. and Baszler, T. V. and Katz, J. B. and Hennager, S. G. and Knowles, D. P.}, year={1999}, pages={2285–2290} }
 @article{perryman_kapil_jones_hunt_1999, title={Protection of calves against cryptosporidiosis with immune bovine colostrum induced by a Cryptosporidium parvum recombinant protein}, volume={17}, ISSN={["0264-410X"]}, DOI={10.1016/S0264-410X(98)00477-0}, abstractNote={The purpose of the study was to determine if immunization with a recombinant protein (rC7) of Cryptosporidium parvum would induce immune bovine colostrum that protected calves against cryptosporidiosis following oral challenge with C. parvum oocysts. Late gestation Holstein cows with low titers of antibody to the p23 antigen of C. parvum were immunized three times with 300 μg affinity purified rC7 C. parvum recombinant protein (immune cows), or left nonimmunized (control cows). Colostrum was obtained from each cow in both groups and partitioned into identical aliquots of pooled immune colostrum or pooled control colostrum. Twelve calves obtained at birth received either immune or control colostrum within the first 2 h, and again at 12 and 24 h of age. Each calf was challenged orally with 107 C. parvum oocysts at 12 h of age and monitored for signs of cryptosporidiosis. All six calves administered pooled control colostrum developed severe diarrhea (mean total fecal volume=8447±5600 ml) and shed an average of 1.87±1.66×1012 C. parvum oocysts. None of the six calves administered pooled immune colostrum developed diarrhea (mean total fecal volume=740±750 ml, p<0.05), and shed significantly fewer oocysts (3.05±2.26×109, p<0.05). The absence of diarrhea and 2.79 log10 (99.8%) reduction in oocyst excretion indicates that immune bovine colostrum induced by immunization with C. parvum recombinant protein rC7 provided substantial protection against cryptosporidiosis in neonatal calves.}, number={17}, journal={VACCINE}, author={Perryman, LE and Kapil, SJ and Jones, ML and Hunt, EL}, year={1999}, month={Apr}, pages={2142–2149} }
 @article{tschetter_davis_perryman_mcguire_1998, title={CD8 dimer usage on alpha beta and gamma delta T lymphocytes from equine lymphoid tissues}, volume={198}, ISSN={["0171-2985"]}, DOI={10.1016/S0171-2985(98)80050-8}, abstractNote={Eight murine monoclonal antibodies (mAb) were used to identify the equine CD8α or CD8β chains and to define the expression of these chains on lymphocytes from various lymphoid tissues. CD8α was a 39 kDa protein and CD8β was a 32 kDa protein. Both chains were expressed on most of the CD8+ T lymphocytes in the peripheral blood, spleen, thymus, mesenteric lymph nodes and ileal intraepithelial lymphocytes (IEL), however, in each lymphoid compartment a percentage of lymphocytes expressed only the CD8α chain. The largest percentage of CD8αα expressing T lymphocytes was 37.7% of the IELs. Purified T lymphocytes from the ileum expressing CD8αβ co-expressed the αβ T cell receptor (TCR). In contrast, purified CD8+ T lymphocytes from the PBMC co-expressed either the αβ or γδ TCR by RT-PCR. Use of pooled anti-CD8α mAb of the murine IgG2a isotype and rabbit complement resulted in lysis of the entire CD8 expressing population in peripheral blood mononuclear cells (PBMC). These results indicated that CD8 dimer usage by equine T lymphocytes is similar to other species and that the mAb described can be further used to separate equine CD8+ T lymphocyte subsets from the lymphoid tissues to define their function in protection against viral and other infections.}, number={4}, journal={IMMUNOBIOLOGY}, author={Tschetter, JR and Davis, WC and Perryman, LE and McGuire, TC}, year={1998}, month={Feb}, pages={424–438} }
 @article{leber_wiler_perryman_meek_1998, title={Equine SCID: mechanistic analysis and comparison with murine SCID}, volume={65}, ISSN={["0165-2427"]}, DOI={10.1016/S0165-2427(98)00174-3}, abstractNote={V(D)J rearrangement is the molecular mechanism by which an almost limitless number of unique immune receptors is generated. V(D)J rearrangement involves two DNA breaks and religations – resulting in two DNA joints; coding and signal joints. If V(D)J recombination is impaired (as in murine SCID (C.B-17 mouse] or RAG [Recombinase Activating Genes) deficient mice), B lymphocyte and T lymphocyte development is blocked and severe immunodeficiency results. The first animal model of SCID was reported in Arabian foals in 1973. Recently we demonstrated that the mechanistic defect in SCID foals is V(D)J recombination. However, the impairment of V(D)J recombination in SCID foals is phenotypically distinct from SCID mice in that both signal and coding joint ligation are impaired. Furthermore, though equine SCID and murine SCID have definite phenotypic differences, both defects are likely to be the result of defective expression of the catalytic subunit of the DNA-dependent protein kinase.}, number={1}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Leber, R and Wiler, R and Perryman, LE and Meek, K}, year={1998}, month={Sep}, pages={1–9} }
 @article{aguirre_perryman_davis_mcguire_1998, title={IL-4 protects adult C57BL/6 mice from prolonged Cryptosporidium parvum infection: Analysis of CD4(+)alpha beta+IFN-gamma(+) and CD4(+)alpha beta+IL-4(+) lymphocytes in gut-associated lymphoid tissue during resolution of infection}, volume={161}, number={4}, journal={Journal of Immunology}, author={Aguirre, S. A. and Perryman, L. E. and Davis, W. C. and McGuire, T. C.}, year={1998}, pages={1891–1900} }
 @article{shin_perryman_meek_1997, title={A kinase-negative mutation of DNA-PK(CS) in equine scid results in defective coding and signal joint formation}, volume={158}, number={8}, journal={Journal of Immunology}, author={Shin, E. K. and Perryman, L. E. and Meek, K.}, year={1997}, pages={3565–3569} }
 @article{wyatt_brackett_perryman_ficht_brown_o'rourke_1997, title={Activation of intestinal intraepithelial T lymphocytes in calves infected with Cryptosporidium parvum}, volume={65}, number={1}, journal={Infection and Immunity}, author={Wyatt, C. R. and Brackett, E. J. and Perryman, L. E. and Ficht, A. C. and Brown, W. C. and O'Rourke, K. I.}, year={1997}, pages={185–190} }
 @article{tschetter_byrne_perryman_mcguire_1997, title={Control of equine infectious anemia virus is not dependent on ADCC mediating antibodies}, volume={230}, ISSN={["0042-6822"]}, DOI={10.1006/viro.1997.8502}, abstractNote={Horses infected with equine infectious anemia virus (EIAV) have recurrent episodes of viremia which are eventually controlled, but the immune mechanisms have not been identified. Antibodies were detected to the surface of EIAV-infected cells within 1 month postinfection and remained for at least 3.5 years postinfection. These antibodies recognized cell surface-exposed envelope (Env) glycoproteins, but could not mediate antibody dependent cellular cytotoxicity (ADCC) using EIAV-WSU5-infected equine kidney (EK) cells as targets and peripheral blood mononuclear cells (PBMC) or polymorphonuclear cells (PMN) as effector cells. Furthermore, purified IgG antibodies from horses infected with either EIAV-WSU5 or EIAV-Wyo did not mediate ADCC of infected target cells. Armed effector cells could not be detected in infected horse blood nor could effector cells be prearmed by incubation with serum antibodies to cell surface antigens. The use of EIAV-WSU5-infected equine macrophages as target cells did not result in ADCC. In contrast, serum antibody from EHV-1 vaccinated horses and PBMC or PMN as effector cells caused ADCC of EHV-1-infected EK cells. These results indicate that ADCC is not involved in the control of EIAV in carrier horses.}, number={2}, journal={VIROLOGY}, author={Tschetter, JR and Byrne, KM and Perryman, LE and McGuire, TC}, year={1997}, month={Apr}, pages={275–280} }
 @article{shin_perryman_meek_1997, title={Evaluation of a test for identification of Arabian horses heterozygous for the severe combined immunodeficiency trait}, volume={211}, number={10}, journal={Journal of the American Veterinary Medical Association}, author={Shin, E. K. and Perryman, L. E. and Meek, K.}, year={1997}, pages={1268-} }
 @article{knowles_kappmeyer_perryman_1997, title={Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species}, volume={90}, number={1}, journal={Molecular and Biochemical Parasitology}, author={Knowles, D. P. and Kappmeyer, L. S. and Perryman, L. E.}, year={1997}, pages={69–79} }
 @article{perryman_jasmer_riggs_bohnet_mcguire_arrowood_1996, title={A cloned gene of Cryptosporidium parvum encodes neutralization-sensitive epitopes}, volume={80}, ISSN={["0166-6851"]}, DOI={10.1016/0166-6851(96)02681-3}, abstractNote={Two mAb, C6B6 and 7D10, each significantly reduced infection of mice by Cryptosporidium parvum and reacted with a 23-kDa glycoprotein (p23) of geographically disperse C. parvum isolates. The antibodies were used to identify plaques in a cDNA library prepared from C. parvum sporozoite mRNA. cDNA insert sequences from positive plaques were determined and used to isolate additional clones encoding p23 coding sequences. A consensus open reading frame of 333 base pairs, encoding 111 amino acids, was identified in this collection of cDNAs. The predicted amino acid sequence contained one N-glycosylation site, but lacked hydrophobic membrane spanning regions. Epitope mapping revealed that mAb 7D10 defines the linear epitope QDKPAD which occurs twice in the C terminal region of the peptide encoded by the ORF. This same C terminal peptide region contains a non-linear epitope bound by mAb C6B6. Serum from mice immunized with synthetic C terminal peptide reacted with sporozoite p23. The occurrence of neutralization-sensitive epitopes encoded by defined regions of the C. parvum genome suggests that recombinant proteins or synthetic peptides containing these epitopes may prove useful for inducing immune responses that diminish infection.}, number={2}, journal={MOLECULAR AND BIOCHEMICAL PARASITOLOGY}, author={Perryman, LE and Jasmer, DP and Riggs, MW and Bohnet, SG and McGuire, TC and Arrowood, MJ}, year={1996}, month={Oct}, pages={137–147} }