@misc{wheeler_steiner_schlarbaum_neale_2015, title={The evolution of forest genetics and tree improvement research in the United States}, volume={113}, number={5}, journal={Journal of Forestry}, author={Wheeler, N. C. and Steiner, K. C. and Schlarbaum, S. E. and Neale, D. B.}, year={2015}, pages={500–510} } @article{fang_blackmon_staton_nelson_kubisiak_olukolu_henry_zhebentyayeva_saski_cheng_et al._2013, title={A physical map of the Chinese chestnut (Castanea mollissima) genome and its integration with the genetic map}, volume={9}, ISSN={["1614-2950"]}, DOI={10.1007/s11295-012-0576-6}, abstractNote={Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www.fagaceae.org/physical_maps .}, number={2}, journal={TREE GENETICS & GENOMES}, author={Fang, Guang-Chen and Blackmon, Barbara P. and Staton, Margaret E. and Nelson, C. Dana and Kubisiak, Thomas L. and Olukolu, Bode A. and Henry, David and Zhebentyayeva, Tatyana and Saski, Christopher A. and Cheng, Chun-Huai and et al.}, year={2013}, month={Apr}, pages={525–537} } @article{kubisiak_nelson_staton_zhebentyayeva_smith_olukolu_fang_hebard_anagnostakis_wheeler_et al._2012, title={A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)}, volume={9}, ISSN={1614-2942 1614-2950}, url={http://dx.doi.org/10.1007/S11295-012-0579-3}, DOI={10.1007/s11295-012-0579-3}, abstractNote={The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, ‘Mahogany’, ‘Nanking’, and ‘Vanuxem’, totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.}, number={2}, journal={Tree Genetics & Genomes}, publisher={Springer Science and Business Media LLC}, author={Kubisiak, T. L. and Nelson, C. D. and Staton, M. E. and Zhebentyayeva, T. and Smith, C. and Olukolu, B. A. and Fang, G.-C. and Hebard, F. V. and Anagnostakis, S. and Wheeler, N. and et al.}, year={2012}, month={Nov}, pages={557–571} } @article{barakat_staton_cheng_park_yassin_ficklin_yeh_hebard_baier_powell_et al._2012, title={Chestnut resistance to the blight disease: Insights from transcriptome analysis}, volume={12}, journal={BMC Plant Biology}, author={Barakat, A. and Staton, M. and Cheng, C. H. and Park, J. and Yassin, N. B. M. and Ficklin, S. and Yeh, C. C. and Hebard, F. and Baier, K. and Powell, W. and et al.}, year={2012} } @article{kremer_sederoff_wheeler_2010, title={Genomics of forest and ecosystem health in the Fagaceae: meeting report}, volume={6}, ISSN={["1614-2942"]}, DOI={10.1007/s11295-010-0277-y}, abstractNote={A summary of 35 keynote, invited and volunteer papers delivered at a recent international conference is provided along with web links to PDFs of those presentations. Major conference themes targeted Genomic Tool Development for the Fagaceae and Application of Genomic Resources. The meeting provided a venue for reviewing the rapidly expanding knowledge base on Fagaceae genomics and for developing collaborations between scientists from Europe and North America.}, number={5}, journal={TREE GENETICS & GENOMES}, author={Kremer, Antoine and Sederoff, Ronald and Wheeler, Nicholas}, year={2010}, month={Oct}, pages={815–820} } @article{barakat_diloreto_zhang_smith_baier_powell_wheeler_sederoff_carlson_2009, title={Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection}, volume={9}, ISSN={["1471-2229"]}, DOI={10.1186/1471-2229-9-51}, abstractNote={American chestnut (Castanea dentata) was devastated by an exotic pathogen in the beginning of the twentieth century. This chestnut blight is caused by Cryphonectria parasitica, a fungus that infects stem tissues and kills the trees by girdling them. Because of the great economic and ecological value of this species, significant efforts have been made over the century to combat this disease, but it wasn't until recently that a focused genomics approach was initiated. Prior to the Genomic Tool Development for the Fagaceae project, genomic resources available in public databases for this species were limited to a few hundred ESTs. To identify genes involved in resistance to C. parasitica, we have sequenced the transcriptome from fungal infected and healthy stem tissues collected from blight-sensitive American chestnut and blight-resistant Chinese chestnut (Castanea mollissima) trees using ultra high throughput pyrosequencing. We produced over a million 454 reads, totaling over 250 million bp, from which we generated 40,039 and 28,890 unigenes in total from C. mollissima and C. dentata respectively. The functions of the unigenes, from GO annotation, cover a diverse set of molecular functions and biological processes, among which we identified a large number of genes associated with resistance to stresses and response to biotic stimuli. In silico expression analyses showed that many of the stress response unigenes were expressed more in canker tissues versus healthy stem tissues in both American and Chinese chestnut. Comparative analysis also identified genes belonging to different pathways of plant defense against biotic stresses that are differentially expressed in either American or Chinese chestnut canker tissues. Our study resulted in the identification of a large set of cDNA unigenes from American chestnut and Chinese chestnut. The ESTs and unigenes from this study constitute an important resource to the scientific community interested in the discovery of genes involved in various biological processes in Chestnut and other species. The identification of many defense-related genes differentially expressed in canker vs. healthy stem in chestnuts provides many new candidate genes for developing resistance to the chestnut blight and for studying pathways involved in responses of trees to necrotrophic pathogens. We also identified several candidate genes that may underline the difference in resistance to Cryphonectria parasitica between American chestnut and Chinese chestnut.}, journal={BMC PLANT BIOLOGY}, author={Barakat, Abdelali and DiLoreto, Denis S. and Zhang, Yi and Smith, Chris and Baier, Kathleen and Powell, William A. and Wheeler, Nicholas and Sederoff, Ron and Carlson, John E.}, year={2009}, month={May} } @article{wheeler_sederoff_2009, title={Role of genomics in the potential restoration of the American chestnut}, volume={5}, ISSN={["1614-2950"]}, DOI={10.1007/s11295-008-0180-y}, number={1}, journal={TREE GENETICS & GENOMES}, author={Wheeler, Nicholas and Sederoff, Ronald}, year={2009}, month={Jan}, pages={181–187} }