@misc{yao_chung_lin_tsai_chang_yeh_tsai_liao_hua_lai_et al._2019, title={Genetic loci determining total immunoglobulin E levels from birth through adulthood}, volume={74}, ISSN={["1398-9995"]}, DOI={10.1111/all.13654}, abstractNote={Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.}, number={3}, journal={ALLERGY}, author={Yao, Tsung-Chieh and Chung, Ren-Hua and Lin, Chung-Yen and Tsai, Pei-Chien and Chang, Wei-Chiao and Yeh, Kuo-Wei and Tsai, Ming-Han and Liao, Sui-Ling and Hua, Man-Chin and Lai, Shen-Hao and et al.}, year={2019}, month={Mar}, pages={621–625} }
 @article{tsai_breen_2012, title={Array-based comparative genomic hybridization-guided identification of reference genes for normalization of real-time quantitative polymerase chain reaction assay data for lymphomas, histiocytic sarcomas, and osteosarcomas of dogs}, volume={73}, ISSN={["0002-9645"]}, DOI={10.2460/ajvr.73.9.1335}, abstractNote={To identify suitable reference genes for normalization of real-time quantitative PCR (RT-qPCR) assay data for common tumors of dogs.Malignant lymph node (n = 8), appendicular osteosarcoma (9), and histiocytic sarcoma (12) samples and control samples of various nonneoplastic canine tissues.Array-based comparative genomic hybridization (aCGH) data were used to guide selection of 9 candidate reference genes. Expression stability of candidate reference genes and 4 commonly used reference genes was determined for tumor samples with RT-qPCR assays and 3 software programs.LOC611555 was the candidate reference gene with the highest expression stability among the 3 tumor types. Of the commonly used reference genes, expression stability of HPRT was high in histiocytic sarcoma samples, and expression stability of Ubi and RPL32 was high in osteosarcoma samples. Some of the candidate reference genes had higher expression stability than did the commonly used reference genes.Data for constitutively expressed genes with high expression stability are required for normalization of RT-qPCR assay results. Without such data, accurate quantification of gene expression in tumor tissue samples is difficult. Results of the present study indicated LOC611555 may be a useful RT-qPCR assay reference gene for multiple tissue types. Some commonly used reference genes may be suitable for normalization of gene expression data for tumors of dogs, such as lymphomas, osteosarcomas, or histiocytic sarcomas.}, number={9}, journal={AMERICAN JOURNAL OF VETERINARY RESEARCH}, author={Tsai, Pei-Chien and Breen, Matthew}, year={2012}, month={Sep}, pages={1335–1343} }
 @article{lin_thomas_tsai_breen_london_2009, title={Generation and characterization of novel canine malignant mast cell line CL1}, volume={127}, ISSN={["1873-2534"]}, DOI={10.1016/j.vetimm.2008.09.027}, abstractNote={Studies using the currently available malignant canine mast cell lines and bone marrow-derived cultured mast cells (BMCMCs) have provided an in-depth understanding of normal and neoplastic canine mast cell biology. However, many of the currently available malignant canine mast cell lines possess limitations, including loss of cell surface markers and inability to bind canine IgE. We have recently generated a novel mast cell line, CL1, from an 11-year-old spayed female Labrador retriever diagnosed with systemic mastocytosis and neoplastic effusion. The CL1 cells express KIT, FcepsilonRI, CD44, CD45, CD14, CD11a, CD11b and CD18 as well as chymase. Interestingly, these cells express wild-type KIT, with no evidence of autophosphorylation, but are able to proliferate independently without the addition of exogenous stem cell factor (SCF), KIT ligand. However, stimulation of CL1 cells with SCF induces KIT phosphorylation promoting cell proliferation. The CL1 cells retain functional properties of mast cells, degranulating in a dose-dependent manner in response to both IgE cross-linking and chemical stimulation. Lastly, cytogenetic evaluation revealed several recurrent tumor-associated chromosome copy number imbalances in the CL1 line. In summary, the CL1 cell line possesses phenotypic and functional properties similar to those found in canine BMCMCs, and will likely be a useful tool to study mast cell biology, factors regulating transformation of mast cells, cytogenetic abnormalities in mast cell tumors, and novel preclinical therapies.}, number={1-2}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Lin, Tzu-Yin and Thomas, Rachael and Tsai, Pei-Chien and Breen, Matthew and London, Cheryl A.}, year={2009}, month={Jan}, pages={114–124} }
 @article{thomas_wang_tsai_langford_fosmire_jubala_getzy_cutter_modiano_breen_2009, title={Influence of genetic background on tumor karyotypes: Evidence for breed-associated cytogenetic aberrations in canine appendicular osteosarcoma}, volume={17}, ISSN={["1573-6849"]}, DOI={10.1007/s10577-009-9028-z}, abstractNote={Recurrent chromosomal aberrations in solid tumors can reveal the genetic pathways involved in the evolution of a malignancy and in some cases predict biological behavior. However, the role of individual genetic backgrounds in shaping karyotypes of sporadic tumors is unknown. The genetic structure of purebred dog breeds, coupled with their susceptibility to spontaneous cancers, provides a robust model with which to address this question. We tested the hypothesis that there is an association between breed and the distribution of genomic copy number imbalances in naturally occurring canine tumors through assessment of a cohort of Golden Retrievers and Rottweilers diagnosed with spontaneous appendicular osteosarcoma. Our findings reveal significant correlations between breed and tumor karyotypes that are independent of gender, age at diagnosis, and histological classification. These data indicate for the first time that individual genetic backgrounds, as defined by breed in dogs, influence tumor karyotypes in a cancer with extensive genomic instability.}, number={3}, journal={CHROMOSOME RESEARCH}, author={Thomas, Rachael and Wang, Huixia J. and Tsai, Pei-Chien and Langford, Cordelia F. and Fosmire, Susan P. and Jubala, Cristan M. and Getzy, David M. and Cutter, Gary R. and Modiano, Jaime F. and Breen, Matthew}, year={2009}, month={Apr}, pages={365–377} }