Works (11)

Updated: July 5th, 2023 15:50

2020 journal article

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

NATURE COMMUNICATIONS, 11(1).

By: P. Dinh n, D. Paudel n, H. Brochu n, K. Popowski n, M. Gracieux n, J. Cores n, K. Huang n, M. Hensley n ...

MeSH headings : Administration, Inhalation; Alveolar Epithelial Cells / drug effects; Alveolar Epithelial Cells / metabolism; Alveolar Epithelial Cells / pathology; Animals; Apoptosis / drug effects; Bleomycin / toxicity; Cell Proliferation; Disease Models, Animal; Exosomes / metabolism; Exosomes / transplantation; Humans; Idiopathic Pulmonary Fibrosis / chemically induced; Idiopathic Pulmonary Fibrosis / metabolism; Idiopathic Pulmonary Fibrosis / pathology; Idiopathic Pulmonary Fibrosis / therapy; Lung / cytology; Lung / metabolism; Lung Injury / chemically induced; Lung Injury / metabolism; Lung Injury / pathology; Lung Injury / therapy; Mesenchymal Stem Cells / metabolism; Mice; Myofibroblasts / cytology; Proteomics; Silicon Dioxide / toxicity; Spheroids, Cellular / metabolism
TL;DR: It is shown that the secretome and exosomes of lung spheroid cells is effective as inhalation treatment in rodent models of lung injury and fibrosis and superior to the counterparts derived from mesenchymal stem cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 23, 2020

2019 journal article

Proteomic method to extract, concentrate, digest and enrich peptides from fossils with coloured (humic) substances for mass spectrometry analyses

ROYAL SOCIETY OPEN SCIENCE, 6(8).

By: E. Schroeter n, K. Blackburn n, M. Goshe n & M. Schweitzer n

author keywords: palaeoproteomics; fossils; humic substances; protein; mass spectrometry; moa
TL;DR: A method combining multiple recent advances in extraction protocols to both concentrate proteins from fossil specimens with high humic content and remove humics, producing clean samples easily analysed by mass spectrometry (MS). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019

2017 journal article

Autophosphorylation-based Calcium (Ca2(+)) Sensitivity Priming and Ca2(+)/Calmodulin Inhibition of Arabidopsis thaliana Ca2(+)-dependent Protein Kinase 28 (CPK28)

JOURNAL OF BIOLOGICAL CHEMISTRY, 292(10), 3988–4002.

By: K. Bender*, R. Blackburn n, J. Monaghan*, P. Derbyshire*, F. Menke*, C. Zipfel*, M. Goshe n, R. Zielinski*, S. Huber*

MeSH headings : Amino Acid Sequence; Arabidopsis / drug effects; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Calcium / pharmacology; Calmodulin / pharmacology; Gene Expression Regulation, Plant / drug effects; Kinetics; Phosphorylation / drug effects; Protein Binding; Protein Conformation; Protein Interaction Domains and Motifs; Protein Kinases / chemistry; Protein Kinases / metabolism; Sequence Homology, Amino Acid
TL;DR: In situ autophosphorylation of CPK28 was demonstrated to have enhanced kinase activity at physiological concentrations of Ca2+ compared with the dephosphorylated protein, suggesting that autoph phosphorylation functions to primeCPK28 forCa2+ activation and might also allow CPK 28 to remain active when Ca2- levels are low. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Quantitative Proteomic Analysis of Human Airway Cilia Identifies Previously Uncharacterized Proteins of High Abundance

JOURNAL OF PROTEOME RESEARCH, 16(4), 1579–1592.

By: K. Blackburn n, X. Bustamante-Marin*, W. Yin*, M. Goshe n & L. Ostrowski*

author keywords: cilia; axoneme; mass spectrometry; proteomics; airway; dynein; tubulin; radial spoke
MeSH headings : Axoneme / genetics; Cilia / genetics; Dyneins / genetics; Dyneins / isolation & purification; Gene Expression Regulation; Humans; Proteins / genetics; Proteins / isolation & purification; Proteome / genetics; Proteomics; Tubulin / genetics; Tubulin / isolation & purification
TL;DR: These studies have provided the first quantitative analysis of the ciliary proteome and have identified and characterized several previously unknown proteins as major constituents of human airway cilia. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Development of a tandem affinity phosphoproteomic method with motif selectivity and its application in analysis of signal transduction networks

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 988, 166–174.

By: L. Herring n, K. Grant*, K. Blackburn n, J. Haugh n & M. Goshe n

author keywords: Phosphopeptide enrichment; Liquid chromatography; Mass spectrometry; Motif analysis; Pathway analysis
MeSH headings : Amino Acid Sequence; Chromatography, Liquid / methods; Molecular Sequence Data; Phosphopeptides / analysis; Phosphopeptides / chemistry; Proteomics / methods; Signal Transduction / physiology; Tandem Mass Spectrometry / methods
TL;DR: A multi-dimensional method incorporating electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) with tandem IMAC/TiO2 enrichment for subsequent phosphopeptide identification by LC/MS/MS and biological functions that were over-represented at each PDGF stimulation time point illustrate the feasibility of this approach in quantitative phosphoproteomic studies. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

Lengthening the Intersubunit Linker of Procaspase 3 Leads to Constitutive Activation

BIOCHEMISTRY, 52(36), 6219–6231.

By: S. MacKenzie n, J. Schipper n, E. England n, M. Thomas n, K. Blackburn n, P. Swartz n, A. Clark n

MeSH headings : Apoptosis / physiology; Caspase 3 / chemistry; Caspase 3 / genetics; Caspase 3 / metabolism; Caspase Inhibitors / pharmacology; Catalytic Domain; Enzyme Activation; Enzyme Precursors / chemistry; Models, Chemical; Models, Molecular; Molecular Dynamics Simulation; Protein Conformation; Protein Multimerization
TL;DR: The data show that releasing the strain of the short IL is not sufficient to populate the active conformer of the native ensemble, and it is shown that increasing the length of the IL by introducing 3-5 alanines results in constitutively active procaspases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Reversible cyclic peptide libraries for the discovery of affinity ligands

Analytical Chemistry, 85(19), 9229–9237.

By: S. Menegatti n, K. Ward n, A. Naik n, W. Kish n, R. Blackburn n & R. Carbonell n

MeSH headings : Ligands; Molecular Structure; Peptide Library; Peptides, Cyclic / analysis; Tandem Mass Spectrometry
TL;DR: A method is shown for synthesis of the irreversible cyclic peptide corresponding to the proposed depsipeptide structure, to make the ligand stable to the aqueous acid and alkaline conditions encountered in affinity chromatographic applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek's Disease Virus

JOURNAL OF PROTEOME RESEARCH, 11(12), 5663–5677.

By: K. Chien n, K. Blackburn n, H. Liu n & M. Goshe n

author keywords: proteomics; phosphoproteomics; liquid chromatography; mass spectrometry; Marek's Disease Virus; phosphorylation
MeSH headings : Amino Acid Sequence; Animals; Cells, Cultured; Chick Embryo; Chromatography, Affinity / methods; Eukaryotic Initiation Factor-4E / metabolism; Fibroblasts / metabolism; Fibroblasts / virology; Herpesvirus 2, Gallid / classification; Herpesvirus 2, Gallid / pathogenicity; Marek Disease; Mass Spectrometry / methods; Molecular Sequence Data; Multiprotein Complexes / analysis; Multiprotein Complexes / metabolism; Peptide Chain Initiation, Translational; Phosphopeptides / analysis; Phosphopeptides / metabolism; Phosphoproteins / analysis; Phosphoproteins / metabolism; Phosphorylation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Transcription, Genetic; Viral Plaque Assay
TL;DR: A kinase-dependent pathway to regulate phosphorylation of 4E-BP1 to modulate assembly of the protein translation initiation complex was revealed and provided new insight for future functional and biochemical characterization of specific proteins involved in MDV pathogenesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Data-independent liquid chromatography/mass spectrometry (LC/MSE) detection and quantification of the secreted Apium graveolens pathogen defense protein mannitol dehydrogenase

Rapid Communications in Mass Spectrometry, 24(7), 1009–1016.

By: K. Blackburn n, F. Cheng n, J. Williamson n & M. Goshe n

MeSH headings : Amino Acid Sequence; Apium / drug effects; Apium / enzymology; Cell Culture Techniques; Chromatography, Liquid / methods; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Mannitol Dehydrogenases / chemistry; Mannitol Dehydrogenases / genetics; Mannitol Dehydrogenases / metabolism; Molecular Sequence Data; Proteome / analysis; Proteome / drug effects; Proteome / metabolism; Salicylic Acid / pharmacology; Tandem Mass Spectrometry / methods
TL;DR: LC/MS(E) can be implemented as a discovery-driven MRM-based quantitative approach which can be used to reveal potential post-translational modifications, thus providing a new method in the area of gel-free and label-free proteomic analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Improving Protein and Proteome Coverage through Data-Independent Multiplexed Peptide Fragmentation

JOURNAL OF PROTEOME RESEARCH, 9(7), 3621–3637.

By: K. Blackburn n, F. Mbeunkui n, S. Mitra n, T. Mentzel n & M. Goshe n

author keywords: Proteomics; mass spectrometry; protein characterization; data-independent acquisition (DIA); data-dependent acquisition (DDA); protein identification; LC/MSE
MeSH headings : Amino Acid Sequence; Animals; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Artifacts; Cattle; Chromatography, Liquid / methods; Ions / chemistry; Solanum lycopersicum; Mass Spectrometry / methods; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Plant Leaves; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Kinases / chemistry; Protein Kinases / metabolism; Proteomics / methods; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / metabolism; Trypsin / metabolism
TL;DR: The fundamental limitations of serial MS/MS interrogation are revealed and the advantages of parallel fragmentation by DIA for more comprehensive protein identification and characterization are revealed which holds promise for enhanced isoform and post-translational modification analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Mass Spectrometry Bioinformatics: Tools for Navigating the Proteomics Landscape

CURRENT ANALYTICAL CHEMISTRY, 5(2), 131–143.

By: K. Blackburn* & M. Goshe

author keywords: Mass spectrometry; Peptide identification; Product ion spectrum; Database searching; Proteomics; Bioinformatics
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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