@article{hansen_petracek_dickey_thompson_2001, title={The 5 ' end of the pea ferredoxin-1 mRNA mediates rapid and reversible light-directed changes in translation in tobacco}, volume={125}, ISSN={["0032-0889"]}, DOI={10.1104/pp.125.2.770}, abstractNote={Ferredoxin-1 (Fed-1) mRNA contains an internal light response element (iLRE) that destabilizes mRNA when light-grown plants are placed in darkness. mRNAs containing this element dissociate from polyribosomes in the leaves of transgenic tobacco (Nicotiana tabacum) plants transferred to the dark for 2 d. Here, we report in vivo labeling experiments with a chloramphenicol acetyl transferase mRNA fused to the Fed-1 iLRE. Our data indicate that the Fed-1 iLRE mediates a rapid decline in translational efficiency and that iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred to darkness. Both events occur before the decline in mRNA abundance, and polyribosome association is rapidly reversible if plants are re-illuminated. These observations support a model in which Fed-1 mRNA in illuminated leaves is stabilized by its association with polyribosomes, and/or by translation. In darkness a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded. We also show that a significant portion of total tobacco leaf mRNA is shifted from polyribosomal to non-polyribosomal fractions after 20 min in the dark, indicating that translation of other mRNAs is also rapidly down-regulated in response to darkness. This class includes some, but not all, cytoplasmic mRNAs encoding proteins involved in photosynthesis.}, number={2}, journal={PLANT PHYSIOLOGY}, author={Hansen, ER and Petracek, ME and Dickey, LF and Thompson, WF}, year={2001}, month={Feb}, pages={770–778} }
 @article{ling_wells_tanguay_dickey_thompson_gallie_2000, title={Heat shock protein HSP101 binds to the Fed-1 internal light regulatory element and mediates its high translational activity}, volume={12}, ISSN={["1532-298X"]}, DOI={10.1105/tpc.12.7.1213}, abstractNote={The internal light-regulatory element (iLRE) of ferredoxin (Fed-1) mRNA, comprising the 5' leader and at least the first 13 codons of the open reading frame, controls transcript abundance after illumination of the plant in a translation-dependent manner. We have characterized the RNA binding activities associated with the Fed-1 iLRE and have identified one activity as the heat shock protein HSP101, a protein shown to bind the 5' leader of tobacco mosaic virus. HSP101 was sufficient and necessary to mediate a high level of translational activity from a Fed-1 iLRE-containing mRNA in yeast. Moreover, the Fed-1 iLRE substantially enhanced translation of reporter mRNAs in plant protoplasts expressing HSP101. Expression of HSP101 was subject to developmental regulation in leaves in that expression was highest in young leaves. These data suggest that Fed-1 mRNA may use the HSP101 regulatory mechanism as a means of ensuring a high level of translation required for the light-mediated regulation of Fed-1 mRNA stability.}, number={7}, journal={PLANT CELL}, author={Ling, J and Wells, DR and Tanguay, RL and Dickey, LF and Thompson, WF and Gallie, DR}, year={2000}, month={Jul}, pages={1213–1227} }
 @article{petracek_nuygen_thompson_dickey_2000, title={Premature termination codons destabilize ferredoxin-1 mRNA when ferredoxin-1 is translated}, volume={21}, ISSN={["1365-313X"]}, DOI={10.1046/j.1365-313x.2000.00705.x}, abstractNote={Ferredoxin-1 (Fed-1) mRNA is poorly translated in dark-treated tobacco (Nicotiana tabacum) leaves, resulting in destabilization of Fed-1 mRNA and a differential light/dark accumulation of the mRNA. Insertion of nonsense codons within the Fed-1 coding sequence disrupts the light regulation of Fed-1 mRNA abundance. Here we show that the nonsense codon effect results primarily from lowering the Fed-1 mRNA stability in light-treated leaf tissue and in rapidly growing tobacco cell cultures, but not in dark-treated leaf tissue. These results suggest that nonsense codons trigger a decay pathway distinct from that seen for Fed-1 mRNA in the dark. We propose that nonsense-mediated decay of nonsense-containing Fed-1 mRNA occurs in light-treated leaves and in non-photosynthetic tobacco culture cells where Fed-1 mRNA is being actively translated.}, number={6}, journal={PLANT JOURNAL}, author={Petracek, ME and Nuygen, T and Thompson, WF and Dickey, LF}, year={2000}, month={Mar}, pages={563–569} }
 @article{petracek_dickey_nguyen_gatz_sowinski_allen_thompson_1998, title={Ferredoxin-1 mRNA is destabilized by changes in photosynthetic electron transport}, volume={95}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.95.15.9009}, abstractNote={In transgenic tobacco, pea Ferredoxin-1 ( Fed-1 ) mRNA accumulates rapidly in response to photosynthesis even when the transgene is driven by a constitutive promoter. To investigate the role of photosynthesis on Fed-1 mRNA stability, we used the tetracycline repressible Top10 promoter system to specifically shut off transcription of the Fed-1 transgene. The Fed-1 mRNA has a half-life of approximately 2.4 hr in the light and a half-life of only 1.2 hr in the dark or in the presence of the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). These data indicate that cessation of photosynthesis, either by darkness or DCMU results in a destabilization of the Fed-1 mRNA. Furthermore, the Fed-1 mRNA half-life is reduced immediately upon transfer to darkness, suggesting that Fed-1 mRNA destabilization is a primary response to photosynthesis rather than a secondary response to long-term dark adaptation. Finally, the two different methods for efficient tetracycline delivery reported here generally should be useful for half-life measurements of other mRNAs in whole plants.}, number={15}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Petracek, ME and Dickey, LF and Nguyen, TT and Gatz, C and Sowinski, DA and Allen, GC and Thompson, WF}, year={1998}, month={Jul}, pages={9009–9013} }
 @article{dickey_petracek_nguyen_hansen_thompson_1998, title={Light regulation of Fed-1 mRNA requires an element in the 5' untranslated region and correlates with differential polyribosome association}, volume={10}, DOI={10.2307/3870603}, number={3}, journal={Plant Cell}, author={Dickey, L. F. and Petracek, M. E. and Nguyen, T. T. and Hansen, E. R. and Thompson, William}, year={1998}, pages={475–484} }
 @inbook{petracek_dickey_hansen_sowinski_nguyen_allen_thompson_1998, title={The dependence of Fed-1 light regulation on translation}, booktitle={A look beyond transcription: Mechanisms determining mRNAstability and translation in plants}, publisher={Washington, DC: American Society of Plant Physiologists}, author={Petracek, M. E. and Dickey, L. F. and Hansen, E. R. and Sowinski, D. A. and Nguyen, T. and Allen, G. C. and Thompson, G. F.}, editor={J. Bailey-Serres and Gallie, D. R.Editors}, year={1998}, pages={96–101} }
 @article{petracek_dickey_huber_thompson_1997, title={Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis}, volume={9}, DOI={10.2307/3870586}, number={12}, journal={Plant Cell}, author={Petracek, M. E. and Dickey, L. F. and Huber, S. C. and Thompson, William}, year={1997}, pages={2291–2300} }
 @article{dickey_nguyen_allen_thompson_1994, title={Light modulation of ferredoxin mRNA abundance requires an open reading frame}, volume={6}, number={8}, journal={Plant Cell}, author={Dickey, L. F. and Nguyen, T. T. and Allen, G. C. and Thompson, W. F.}, year={1994}, pages={1171} }
 @article{dickey_gallo-meagher_thompson_1992, title={Light regulatory sequences are located within the 5' portion of the Fed-1 message sequence}, volume={11}, number={6}, journal={EMBO Journal}, author={Dickey, L. F. and Gallo-Meagher, M. and Thompson, W. F.}, year={1992}, pages={2311} }