@article{kretschmar_cabrera_bradley_roe_2013, title={Novel adult feeding disruption test (FDT) to detect insecticide resistance of lepidopteran pests in cotton}, volume={69}, ISSN={["1526-4998"]}, DOI={10.1002/ps.3420}, abstractNote={BACKGROUND
Resistance monitoring is an important aspect of insect resistance management and the preservation of insecticide efficacy. The adult vial test (AVT) is most often used for resistance monitoring for a variety of insects. A potential alternative method is feeding disruption where resistant insects are distinguished from susceptible insects on the basis of their ability to feed on insecticide in nectar containing a colorimetric marker to measure feeding. The advantages of a feeding disruption test (FDT) for lepidopteran adults might include a more rapid assay than AVT, an assay format easier to prepare, a bioassay applicable to both oral and contact insecticides and the provision of food and water during the course of the test. The objective of the present work was to determine the feasibility of an adult FDT.


RESULTS
Heliothis virescens moths fed permethrin and spinosad in dyed nectar yielded dose-dependent ingestion, fecal production and mortality data. A permethrin diagnostic dose distinguished pyrethroid-resistant from pyrethroid-susceptible moths, based on fecal production.


CONCLUSION
Proof of concept was demonstrated for an adult FDT in which resistant moths were distinguished from susceptible moths on the basis of the ability of the insect to feed on insecticide in dyed nectar and produce dyed feces.}, number={5}, journal={PEST MANAGEMENT SCIENCE}, author={Kretschmar, B. and Cabrera, Ana R. and Bradley, Julius R. and Roe, R. Michael}, year={2013}, month={May}, pages={652–660} }
 @article{cabrera_donohue_khalil_scholl_opperman_sonenshine_roe_2011, title={New approach for the study of mite reproduction: The first transcriptome analysis of a mite, Phytoseiulus persimilis (Acari: Phytoseiidae)}, volume={57}, ISSN={0022-1910}, url={http://dx.doi.org/10.1016/j.jinsphys.2010.09.006}, DOI={10.1016/j.jinsphys.2010.09.006}, abstractNote={Many species of mites and ticks are of agricultural and medical importance. Much can be learned from the study of transcriptomes of acarines which can generate DNA-sequence information of potential target genes for the control of acarine pests. High throughput transcriptome sequencing can also yield sequences of genes critical during physiological processes poorly understood in acarines, i.e., the regulation of female reproduction in mites. The predatory mite, Phytoseiulus persimilis, was selected to conduct a transcriptome analysis using 454 pyrosequencing. The objective of this project was to obtain DNA-sequence information of expressed genes from P. persimilis with special interest in sequences corresponding to vitellogenin (Vg) and the vitellogenin receptor (VgR). These genes are critical to the understanding of vitellogenesis, and they will facilitate the study of the regulation of mite female reproduction. A total of 12,556 contiguous sequences (contigs) were assembled with an average size of 935bp. From these sequences, the putative translated peptides of 11 contigs were similar in amino acid sequences to other arthropod Vgs, while 6 were similar to VgRs. We selected some of these sequences to conduct stage-specific expression studies to further determine their function.}, number={1}, journal={Journal of Insect Physiology}, publisher={Elsevier BV}, author={Cabrera, Ana R. and Donohue, Kevin V. and Khalil, Sayed M.S. and Scholl, Elizabeth and Opperman, Charles and Sonenshine, Daniel E. and Roe, R. Michael}, year={2011}, month={Jan}, pages={52–61} }
 @article{cabrera_van kretschmar_bacheler_burrack_sorenson_roe_2011, title={Resistance monitoring of Heliothis virescens to pyramided cotton varieties with a hydrateable, artificial cotton leaf bioassay}, volume={30}, ISSN={["0261-2194"]}, DOI={10.1016/j.cropro.2011.05.005}, abstractNote={Proof of concept was demonstrated for a practical, off-the-shelf bioassay to monitor for tobacco budworm resistance to pyramided Bt cotton using plant filtrates. The bioassay was based on a previously described feeding disruption test using hydrateable artificial diet containing a blue indicator dye, a diagnostic dose of insecticide and novel assay architecture. Using neonate larvae from a Bt-susceptible, laboratory reared tobacco budworm strain, a diagnostic dose for Bollgard II and WideStrike cotton was obtained that limited neonate blue fecal production to 0–2 pellets in 24 h (Bt-resistant larvae produced >2 fecal pellets). The bioassay was tested with three different field populations of tobacco budworm collected from tobacco in central North Carolina (USA) and shown to accurately diagnose susceptibility to Bt. The diagnostic doses were also successfully evaluated with two Bt-resistant, laboratory reared tobacco budworm strains. Shelf life studies showed the assay could be stored for at least 6 months at room temperature (longer storage times were not studied). The application of the bioassay as an easy to use monitoring tool is discussed.}, number={9}, journal={CROP PROTECTION}, author={Cabrera, Ana R. and Van Kretschmar, Jaap and Bacheler, Jack S. and Burrack, Hannah and Sorenson, Clyde E. and Roe, R. Michael}, year={2011}, month={Sep}, pages={1196–1201} }
 @misc{cabrera_donohue_roe_2009, title={Regulation of female reproduction in mites: A unifying model for the Acari}, volume={55}, ISSN={["1879-1611"]}, DOI={10.1016/j.jinsphys.2009.08.007}, abstractNote={It is well established in the literature that circulating high levels of juvenile hormone (JH) are responsible for the initiation of vitellogenesis and female reproduction in most insects studied so far. Exceptions include some Diptera, Lepidoptera and Hymenoptera. The current view is that JH also regulates yolk protein (vitellogenin, Vg) synthesis and female reproduction in mites. However, there is no published evidence that mites have the common insect JHs at any stage of their development. Also, research on the effects of exogenous applications of JH and JH analogs on the reproduction of mites is contradictory. Significant information is available on the life history of mite reproduction, and new information has become available on mite storage proteins including Vg. Although initial studies suggested that ticks may respond to exogenously applied juvenile hormone or anti-JHs, current research shows that ticks cannot synthesize the common insect JHs and have no detectable levels of these hormones in their hemolymph during female reproduction. In ticks, it appears that ecdysteroids, and not JH, regulate expression of the Vg gene and the synthesis and release of Vg protein into the hemolymph. In fact within the Arthropoda, JH has been found only in insects. Methyl farnesoate and not JH regulates Vg synthesis in the Crustacea, the sister group to the insects. Based on this evidence, a new working hypothesis is proposed, i.e., that ecdysteroids and not the JHs regulate vitellogenesis in the Acari including both ticks and mites. To the present, the role of neuropeptides in the regulation of female reproduction in mites is not known.}, number={12}, journal={JOURNAL OF INSECT PHYSIOLOGY}, author={Cabrera, Ana R. and Donohue, Kevin V. and Roe, R. Michael}, year={2009}, month={Dec}, pages={1079–1090} }