@article{tiruthani_mischler_ahmed_mahinthakumar_haugh_rao_2019, title={Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation}, volume={12}, ISSN={["1937-9145"]}, DOI={10.1126/scisignal.aap7584}, abstractNote={Engineered biosensors accurately report the kinetics of EGFR activation.}, number={584}, journal={SCIENCE SIGNALING}, author={Tiruthani, Karthik and Mischler, Adam and Ahmed, Shoeb and Mahinthakumar, Jessica and Haugh, Jason M. and Rao, Balaji M.}, year={2019}, month={Jun} }
 @article{cruz-teran_tiruthani_mischler_rao_2017, title={Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae}, volume={6}, ISSN={["2161-5063"]}, DOI={10.1021/acssynbio.7b00144}, abstractNote={The need for recombinant expression of soluble protein slows the validation of engineered proteins isolated from combinatorial libraries and limits the number of protein variants evaluated. To overcome this bottleneck, we describe a system for simultaneous cell surface display and soluble secretion of proteins in Saccharomyces cerevisiae based on inefficient ribosomal skipping. Ribosomal skipping mediated by "self-cleaving" 2A peptides produces two proteins from a single open reading frame. Incorporation of the F2A peptide sequence-with ∼50% efficiency of ribosomal skipping-between the protein of interest and the yeast cell wall protein Aga2 results in simultaneous expression of both the solubly secreted protein and the protein-Aga2 fusion that is tethered to the yeast cell surface. We show that binding proteins derived from the Sso7d scaffold and the homodimeric enzyme glucose oxidase can be simultaneously secreted solubly and expressed as yeast cell surface fusions using the F2A-based system. Furthermore, a combinatorial library of Sso7d mutants can be screened to isolate binders with higher affinity for a model target (lysozyme), and the pool of higher affinity binders can be characterized in soluble form. Significantly, we show that both N- and C-terminal fusions to Aga2 can be simultaneously secreted solubly and displayed on the cell surface; this is particularly advantageous because protein functionality can be affected by the specific position of Aga2 in the protein fusion. We expect that the F2A-based yeast surface display and secretion system will be a useful tool for protein engineering and enable efficient characterization of individual clones isolated from combinatorial libraries.}, number={11}, journal={ACS SYNTHETIC BIOLOGY}, author={Cruz-Teran, Carlos A. and Tiruthani, Karthik and Mischler, Adam and Rao, Balaji M.}, year={2017}, month={Nov}, pages={2096–2107} }
 @article{ramasubramanian_tiruthani_2009, title={A Capacitive Displacement Sensing Technique for Early Detection of Unbalanced Loads in a Washing Machine}, volume={9}, ISSN={["1424-8220"]}, DOI={10.3390/s91209559}, abstractNote={Horizontal axis washing machines are water and energy efficient and becoming popular in the USA. Unlike a vertical axis washer, these do not have an agitator and depend solely on tumbling for the agitation of laundry during the wash cycle. However, due to the constant shifting of laundry during washing, the load distribution is often unbalanced during the high speed spin cycle. We present a displacement-based sensing method to detect unbalance early while the spin rate (rpm) is well below the resonance frequency so that corrective actions may be taken prior to the high speed spin cycle. Experimental and analytical characterizations of the sensor configuration are presented. Results show that the displacement sensor is more appropriate than an accelerometer for this application and offer the potential for a simple, reliable, low cost detection of unbalance.}, number={12}, journal={SENSORS}, author={Ramasubramanian, Melur K. and Tiruthani, Karthik}, year={2009}, month={Dec}, pages={9559–9571} }