@article{derocco_anderson_piehler_erie_weninger_2010, title={Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes}, volume={49}, ISSN={["1940-9818"]}, DOI={10.2144/000113551}, abstractNote={ To enable studies of conformational changes within multimolecular complexes, we present a simultaneous, four-color single molecule fluorescence methodology implemented with total internal reflection illumination and camera-based, wide-field detection. We further demonstrate labeling histidine-tagged proteins noncovalently with Tris–nitrilotriacetic acid (Tris-NTA)–conjugated dyes to achieve single molecule detection. We combine these methods to colocalize the mismatch repair protein MutSα on DNA while monitoring MutSα-induced DNA bending using Förster resonance energy transfer (FRET) and to monitor assembly of membrane-tethered SNARE protein complexes. }, number={5}, journal={BIOTECHNIQUES}, author={DeRocco, Vanessa C. and Anderson, Trevor and Piehler, Jacob and Erie, Dorothy A. and Weninger, Keith}, year={2010}, month={Nov}, pages={807-+} }