Works (3)
Updated: July 5th, 2023 16:02
2001 journal article
Arrangement of the central pseudoknot region of 16S rRNA in the 30S ribosomal subunit determined by site-directed 4-thiouridine crosslinking
RNA, 7(1), 71–84.
author keywords: 16S rRNA three-dimensional structure; intramolecular RNA-RNA crosslinks; mRNA decoding region
MeSH headings : Base Sequence; Cross-Linking Reagents; DNA Ligases / metabolism; DNA, Single-Stranded / chemistry; Escherichia coli / genetics; Escherichia coli / virology; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Oligoribonucleotides / chemical synthesis; Oligoribonucleotides / chemistry; RNA, Ribosomal, 16S / chemistry; RNA, Ribosomal, 16S / drug effects; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes / chemistry; Ribosomes / drug effects; Ribosomes / ultrastructure; Thiouridine / pharmacology; Transcription, Genetic
TL;DR:
Results indicate that part of the central pseudoknot is in close contact with the decoding region, with helix 27 in the 885-912 interval and with part of domain III RNA.
(via Semantic Scholar)
open access via europepmc.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 journal article
Short-range RNA-RNA crosslinking methods to determine rRNA structure and interactions
METHODS, 25(3), 333–343.
MeSH headings : Cross-Linking Reagents / pharmacology; DNA Ligases / chemistry; DNA Ligases / isolation & purification; DNA, Complementary / chemistry; Electrophoresis, Polyacrylamide Gel; Models, Molecular; Nucleic Acid Conformation; RNA / chemistry; RNA / ultrastructure; RNA, Messenger / chemistry; RNA, Ribosomal / chemistry; RNA, Ribosomal, 16S / chemistry; RNA, Transfer / chemistry; Ultraviolet Rays
TL;DR:
Details of procedures to analyze RNA-RNA crosslinks made by far-UV irradiation (< 300 nm) or made by irradiation with near-UV light on RNA containing photosensitive nucleotides, in the present case containing 4-thiouridine are described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Organization of the 16S rRNA around its 5 ' terminus determined by photochemical crosslinking in the 30S ribosomal subunit
RNA, 6(1), 26–40.
author keywords: 16S rRNA three-dimensional structure; intramolecular RNA-RNA cross-links; reverse transcription arrest assay
MeSH headings : Azides; Base Sequence; Cross-Linking Reagents; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Ribosomal, 16S / chemistry; RNA, Transfer / chemistry; RNA, Transfer / radiation effects; Ultraviolet Rays
TL;DR:
Differences in the pattern and efficiency of crosslinking for the different constructs allow distance estimates for the crosslinked sites from nucleotide G9 to provide constraints for the arrangement of the RNA elements in the 30S subunit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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