@article{chittick_stoskopf_law_overstreet_levine_2001, title={Evaluation of potential health risks to Eastern Elliptio (Elliptio complanata) (Mollusca: Bivalvia: Unionida: Unionidae) and implications for sympatric endangered freshwater mussel species}, volume={9}, ISBN={1386-1980}, number={1}, journal={Journal of Aquatic Ecosystem Stress and Recovery}, author={Chittick, B. and Stoskopf, M. and Law, M. and Overstreet, R. and Levine, J.}, year={2001}, pages={35} } @article{lewbart_stoskopf_losordo_geyer_owen_smith_law_altier_1999, title={Safety and efficacy of the Environmental Products Group Masterflow Aquarium Management System with Aegis Microbe Shield (TM)}, volume={19}, ISSN={["0144-8609"]}, DOI={10.1016/S0144-8609(98)00043-0}, abstractNote={This study investigated the safety and efficacy of the EPG Masterflow Aquarium Management System with Aegis Microbe Shield™ (EPG-MAMS). Four different species of fish were used in the study. Ten fish of each species were placed in 75 l aquariums containing the EPG filter media, a commercially available filter media (Whisper®) and an aquarium with no filter material. At the end of the 45 day trial three fish from each tank were sacrificed and preserved in formalin for histopathology. Water quality parameters were routinely monitored. The EPG filter media was compared with the Whisper® filter media for efficacy against Aeromonas salmonicida using a shaker flask microbiological assay. The EPG filter proved to be clinically and histopathologically safe and reduced to some degree the number of A. salmonicida suspended in water in an in vitro study.}, number={2}, journal={AQUACULTURAL ENGINEERING}, author={Lewbart, GA and Stoskopf, MK and Losordo, T and Geyer, J and Owen, J and Smith, DW and Law, M and Altier, C}, year={1999}, month={Jan}, pages={93–98} } @article{law_speck_moyer_1992, title={THE M RNA OF IMPATIENS NECROTIC SPOT TOSPOVIRUS (BUNYAVIRIDAE) HAS AN AMBISENSE GENOMIC ORGANIZATION}, volume={188}, ISSN={["0042-6822"]}, DOI={10.1016/0042-6822(92)90528-W}, abstractNote={The nucleotide sequence of Impatiens necrotic spot virus (INSV) M RNA was determined from cDNA clones. The INSV M RNA was 4972 nucleotides in length with two open reading frames (ORFs) in an ambisense genomic organization. The larger ORF near the 3' end of the viral RNA, coding for a protein with a predicted molecular weight of 124.9 kDa, was in the viral complementary sense and produced the G2 and G1 proteins. A smaller ORF in the viral sense was capable of coding for a 34.1-kDa polypeptide, designated the NSm protein. Two subgenomic RNA species were detected in INSV-infected tissue that corresponded to the predicted sizes (3.3 and 1.0 kb) of the G2-G1 and NSm mRNAs. The ORFs were separated by a 478 nucleotide A-U-rich intergenic region similar to the regions found in other viral RNAs with ambisense ORFs. The intergenic region was predicted to form a stable stem-loop structure (-81.2 kcal/mole). The ambisense genomic organization is characteristic of the S RNA for members of the Phlebovirus, Uukuvirus, and Tospovirus genera in the Bunyaviridae family. This is the first report of an ambisense Bunyaviridae M RNA.}, number={2}, journal={VIROLOGY}, author={LAW, MD and SPECK, J and MOYER, JW}, year={1992}, month={Jun}, pages={732–741} } @article{law_speck_moyer_1991, title={NUCLEOTIDE-SEQUENCE OF THE 3' NONCODING REGION AND N-GENE OF THE S RNA OF A SEROLOGICALLY DISTINCT TOSPOVIRUS}, volume={72}, ISSN={["0022-1317"]}, DOI={10.1099/0022-1317-72-10-2597}, abstractNote={A tomato spotted wilt-like virus (TSWV-I) is a distinct member of the Tospovirus genus of the Bunyaviridae and is distinguished from the typical TSWV by having a serologically distinct nucleoprotein (N). A cDNA clone extending from the 3' terminus of the viral RNA through the entire N open reading frame (ORF) was sequenced. The TSWV-I N ORF is capable of encoding a polypeptide of 262 amino acids with a predicted Mr of 28.8K. In vitro transcription and translation of the clone produced a protein which comigrated with TSWV-I N and was immunoprecipitated by TSWV-I antibodies. Hybridization analysis of lithium chloride-precipitated RNA from healthy and TSWV-I-infected tissue detected a virus-specific 1.2 kb subgenomic RNA. The TSWV-I S RNA terminal consensus sequence (8 nucleotides) was identical to that of TSWV; the remaining TSWV-I untranslated region showed only 51% identity with that of TSWV. Comparison of the TSWV-I and TSWV N proteins showed 67% identity at the amino acid level. The degree of similarity in the terminal sequence, untranslated region and N ORF is similar to that expected between distinct serogroups within certain genera of the Bunyaviridae.}, journal={JOURNAL OF GENERAL VIROLOGY}, author={LAW, MD and SPECK, J and MOYER, JW}, year={1991}, month={Oct}, pages={2597–2601} } @article{law_moyer_1990, title={A TOMATO SPOTTED WILT-LIKE VIRUS WITH A SEROLOGICALLY DISTINCT N-PROTEIN}, volume={71}, ISSN={["0022-1317"]}, DOI={10.1099/0022-1317-71-4-933}, abstractNote={A serologically distinct member of the tomato spotted wilt virus (TSWV) group was isolated from the hybrid flower crop New Guinea impatiens (Impatiens sp.) and termed TSWV-I. TSVW-I type isolates have frequently been detected in a wide variety of flower crops throughout the United States. TSWV-I shares many characteristics with TSWV, such as symptomatology and possession of three ssRNA species (L, M and S of 8.3 kb, 5.2 kb and 3.4 kb, respectively) and three structural proteins (G1, G2 and N of 78K, 52K and 28K respectively). The TSWV-I G1 and G2 glycoproteins were serologically related to the respective proteins of TSWV, but the TSWV-I nucleocapsid or N protein was serologically unrelated to that of TSWV. Hybridization analysis under high stringency conditions revealed no hybridization between clones of TSWV-I S and M and the S and M RNAs of TSWV, respectively and in addition, a TSWV S clone hybridized only with TSWV S RNA. The cytopathology of TSWV-I also differed from that of TSWV. TSWV-I-infected tissue primarily contained filamentous structures arranged in paracrystalline arrays, which were also observed by immunosorbent electron microscopy of tissue extracts. The filamentous structures were only trapped by TSWV-I antibodies. The conserved serological relatedness between TSWV types for G1 and G2, but not N, is consistent with serological analyses of the nairovirus and phlebovirus genera of the Bunyaviridae, the virus family that TSWV most closely resembles.}, journal={JOURNAL OF GENERAL VIROLOGY}, author={LAW, MD and MOYER, JW}, year={1990}, month={Apr}, pages={933–938} } @article{law_moyer_payne_1989, title={EFFECT OF HOST-RESISTANCE ON PATHOGENESIS OF MAIZE-DWARF MOSAIC-VIRUS}, volume={79}, ISSN={["0031-949X"]}, DOI={10.1094/Phyto-79-757}, abstractNote={Infectious virus was detected only in tissue above the inoculated leaf in PB3187 plants expressing the susceptible phenotype but not in equivalent leaves of plants expressing the resistant phenotype. This phenomenon of differential phenotype expression was not affected by plant age or temperature. We propose that the resistance mechanism expressed in PB3187 acts at a specific point of systemic virus transport, thereby limiting upward virus transport from the roots to young, developing leaves}, number={7}, journal={PHYTOPATHOLOGY}, author={LAW, MD and MOYER, JW and PAYNE, GA}, year={1989}, month={Jul}, pages={757–761} }