@article{mondorf_kaufman_carbonell_1998, title={Screening of combinatorial peptide libraries: Identification of ligands for affinity purification of proteins using a radiological approach}, volume={52}, DOI={10.1111/j.1399-3011.1998.tb01257.x}, abstractNote={Abstract:Peptides deduced from peptide libraries may serve as affinity ligands for protein purification. Identification of a ligand that binds the protein of interest depends highly on the screening method used. One approach which offers simple and direct detection involves screening a solid‐phase peptide library against a radiolabeled target protein.We have developed a radiological screening method, using 14C as a radioactive label, that offers high resolution and sensitivity. Less than 100 DPM/bead are detectable after a one‐day exposure using autoradiography. The validity of the technique was illustrated by screening a solid‐phase hexameric‐peptide library spiked with YNFEVL‐beads against 14C‐labeled ribonuclease S‐protein. For this particular system, the amount of protein bound to a single bead was estimated to be in the femtomolar range with a peptide:protein ratio of 500:1.Finally, a portion of the library was screened against 14C‐labeled fibrinogen. Three peptides deduced from the library, WQEHYN, WQEHYN, and YENYGY, purified fibrinogen from a mixture with albumin.}, number={6}, journal={Journal of Peptide Research}, author={Mondorf, K. and Kaufman, D. B. and Carbonell, R. G.}, year={1998}, pages={526–536} }
 @misc{mondorf_carbonell_buettner, title={Peptide ligands for affinity purification of fibrinogen}, volume={5,783,663}, number={1998 Jul. 21}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Mondorf, K. and Carbonell, R. C. and Buettner, J. A.} }