@article{chiera_thomas_rufty_2004, title={Growth and localized energy status in phosphorus-stressed soybean}, volume={27}, ISSN={["1532-4087"]}, DOI={10.1081/LPLA-200030007}, abstractNote={Abstract In plants experiencing phosphorus (P) stress, ATP concentrations can be reduced significantly and shoot growth is strongly restricted, raising the possibility that energy availability is responsible for the growth inhibition. Experiments were conducted to investigate the relationship between P deprivation and energy availability in tissues involved in the growth response. Young soybean (Glycine max [L.] Merr. cv. Ransom) plants were deprived of P for 32 days. Leaf initiation and individual leaf expansion were followed along with localized P and ATP concentrations. Tissue analyses revealed preferential distribution of P to the root, which accompanied a decline in the shoot to root dry weight ratio. Even though P concentrations in all shoot tissues dropped sharply, ATP concentrations and energy charge in the shoot meristem region were maintained similar to controls for an extended period when leaf initiation slowed. In the first trifoliolate leaf, ATP and energy charge remained at control levels during the expansion phase, but expansion was inhibited by 50%. Furthermore, ATP levels in root tips were decreased almost 30%, yet growth of the root system was equal to or greater than the control. The absence of a positive correlation between ATP levels and growth responses in the different tissues suggests that energy availability is not a primary factor limiting growth under P stress conditions. The results, along with others from previous experiments, support the notion that a signaling mechanism, as yet unidentified, controls down regulation of cell division in shoot growth regions.}, number={11}, journal={JOURNAL OF PLANT NUTRITION}, author={Chiera, JM and Thomas, JF and Rufty, TW}, year={2004}, pages={1875–1890} }
 @article{chiera_thomas_rufty_2002, title={Leaf initiation and development in soybean under phosphorus stress}, volume={53}, ISSN={["0022-0957"]}, DOI={10.1093/jexbot/53.368.473}, abstractNote={Experiments investigated changes in leaf development in young soybean plants progressing into P stress. The apical meristem and leaf structure were examined anatomically to evaluate the involvement of cell division and cell expansion in the restriction of leaf number and individual leaf size. Seedlings were deprived of P for 32 d following germination. Leaf initiation rates declined noticeably after about 2 weeks, even though the apical dome was of similar size and had a similar number of cells as controls. Primordia appeared morphologically similar also. Expansion of primary and the first three trifoliolate leaves of -P plants was severely reduced, and expansion of each leaf ceased, uniformly, when an area of about 40 cm(2) was obtained. Leaf epidermal cell size in the lateral plane was unaffected. The results indicate that expansion of leaves under P stress was limited by the number of cell divisions, which would imply control of cell division by a common regulatory factor within the leaf canopy.}, number={368}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, author={Chiera, J and Thomas, J and Rufty, T}, year={2002}, month={Mar}, pages={473–481} }