@article{kuznetsov_jiang_2003, title={Bioconvection of negatively geotactic microorganisms in a porous medium: the effect of cell deposition and declogging}, volume={13}, ISSN={["1758-6585"]}, DOI={10.1108/09615530310464535}, abstractNote={Mechanisms of deposition and declogging are considered while formulating a new continuum model for bioconvection in a dilute suspension of motile, negatively geotactic microorganisms in a porous medium. According to research in 1988, bioconvection is the name given to pattern‐forming convective motions set up in suspensions of swimming microorganisms. “Negative geotaxis” means that the microorganisms tend to swim against the gravitational force. This paper is motivated by experimental research by Kessler who investigated the effect of porous media on the development of convection instability in algal suspensions. In the model suggested in this paper, the decrease of permeability due to cell adsorption by the porous medium is considered and the influence of this permeability decrease on the development of bioconvection is studied. The existence and stability of a two‐dimensional plume in a rectangular enclosure with stress‐free sidewalls is investigated. Governing equations include the Darcy law as well as the microorganism conservation equations. A conservative finite‐difference scheme is utilized to solve these equations numerically. The analysis of the proposed model reveals that the major factors affecting the development of bioconvection are the initial permeability of the porous medium and the rate of cell deposition. For small permeability, the resistance to the fluid flow is too large, and bioconvection does not develop. If the rate of cell deposition is too large, the number of suspended cells quickly becomes too small because of cell capturing by the porous medium. For this reason, the critical density difference in the top fluid layer cannot be reached, and bioconvection does not develop.}, number={2-3}, journal={INTERNATIONAL JOURNAL OF NUMERICAL METHODS FOR HEAT & FLUID FLOW}, author={Kuznetsov, AV and Jiang, N}, year={2003}, pages={341–364} }
 @article{jiang_dreher_dye_li_richards_martin_adler_2000, title={Residual oil fly ash induces cytotoxicity and mucin secretion by guinea pig tracheal epithelial cells via an oxidant-mediated mechanism}, volume={163}, ISSN={["0041-008X"]}, DOI={10.1006/taap.1999.8886}, abstractNote={Inhalation of ambient air particulate matter (PM) is associated with pulmonary injury and inflammation. Using primary cultures of guinea pig tracheal epithelial (GPTE) cells as an in vitro model of airway epithelium, we examined effects of exposure to suspensions of six different emission and ambient air PM samples: residual oil fly ash (ROFA) from an electrical power plant; fly ash from a domestic oil burning furnace (DOFA); ambient air dust from St. Louis (STL), Ottawa (OT), and Washington, DC (WDC); and volcanic ash from the eruption of Mount Saint Helens (MSH) in 1980. Effects of these particulates on cell viability (assessed via LDH assay), secretion of mucin (measured by a monoclonal antibody-based ELISA), and steady-state mRNA levels of the mucin gene MUC2 were determined. ROFA was the most toxic of the dusts tested, as it significantly increased LDH release following a 24-h incubation with 50 microg/cm(2) ROFA. ROFA also enhanced MUC2 mRNA after 4-h exposure, and mucin secretion after 8 h. ROFA-induced mucin secretion and cytotoxicity were attenuated by the oxidant scavenger, dimethylthiourea (DMTU). ROFA exposure also depleted cells of glutathione (GSH). Relatedly, depletion of intracellular GSH by treatment of the cells with buthionine sulfoxamine (BSO) also provoked mucin secretion, as well as enhancing the secretory effect of ROFA when the two agents were added together. L-NMA, the nitric oxide synthase (NOS) inhibitor, did not affect ROFA-induced mucin secretion. Of the soluble transition metals in ROFA (nickel, iron, vanadium), only vanadium individually, or combinations of the metals containing vanadium, provoked secretion. The results suggest ROFA enhances mucin secretion and generates toxicity in vitro to airway epithelium via a mechanism(s) involving generation of oxidant stress, perhaps related to depletion of cellular antioxidant capacity. Deleterious effects of inhalation of ROFA in the respiratory tract in vivo may relate to these cellular responses. Vanadium, a component of ROFA, may be important in generating these reactions.}, number={3}, journal={TOXICOLOGY AND APPLIED PHARMACOLOGY}, author={Jiang, NF and Dreher, KL and Dye, JA and Li, YH and Richards, JH and Martin, LD and Adler, KB}, year={2000}, month={Mar}, pages={221–230} }