@article{mphahlele_isik_mostert-o'neill_reynolds_hodge_myburg_2020, title={Expected benefits of genomic selection for growth and wood quality traits inEucalyptus grandis}, volume={16}, ISSN={["1614-2950"]}, DOI={10.1007/s11295-020-01443-1}, number={4}, journal={TREE GENETICS & GENOMES}, author={Mphahlele, Makobatjatji M. and Isik, Fikret and Mostert-O'Neill, Marja M. and Reynolds, S. Melissa and Hodge, Gary R. and Myburg, Alexander A.}, year={2020}, month={Jun} } @article{payn_dvorak_janse_myburg_2008, title={Microsatellite diversity and genetic structure of the commercially important tropical tree species Eucalyptus urophylla, endemic to seven islands in eastern Indonesia}, volume={4}, ISSN={["1614-2950"]}, DOI={10.1007/s11295-007-0128-7}, number={3}, journal={TREE GENETICS & GENOMES}, author={Payn, Kitt G. and Dvorak, William S. and Janse, Bernard J. H. and Myburg, Alexander A.}, year={2008}, month={Jul}, pages={519–530} } @article{payn_dvorak_myburg_2007, title={Chloroplast DNA phylogeography reveals the island colonisation route of Eucalyptus urophylla (Myrtaceae)}, volume={55}, ISSN={0067-1924}, url={http://dx.doi.org/10.1071/bt07056}, DOI={10.1071/BT07056}, abstractNote={We present a study of the colonisation patterns of a tropical tree species among an island archipelago. Eucalyptus urophylla (S.T.Blake) is an economically important plantation species endemic to the volcanic slopes of seven islands in eastern Indonesia. In the present study, we investigated the geographical distribution of chloroplast DNA sequence variation in E. urophylla to gain insight into its historical seed-migration routes. DNA sequence data were obtained from 198 plants from which 20 haplotypes were identified. A moderate to high level of chloroplast genetic differentiation (GST = 0.581, NST = 0.724) and significant phylogeographic structure (NST > GST; P < 0.01) were observed, suggesting low levels of recurrent seed-mediated gene flow among the islands. The highest levels of haplotype diversity were observed on the eastern islands of Wetar and Timor. The two most westerly islands, Flores and Lomblen, were fixed for what appeared to be the ancestral haplotype. Chloroplast haplotype diversity therefore exhibited a decreasing trend from east to west in the species’ range, consistent with an east-to-west colonisation route across the seven islands. Environmental factors that may have contributed to the contemporary spatial distribution of chloroplast DNA haplotypes include island paleogeology, ocean currents, fluctuations in sea levels and possible hybridisation events.}, number={7}, journal={Australian Journal of Botany}, publisher={CSIRO Publishing}, author={Payn, Kitt G. and Dvorak, William S. and Myburg, Alexander A.}, year={2007}, pages={673} } @article{kirst_basten_myburg_zeng_sederoff_2005, title={Genetic architecture of transcript-level variation in differentiating xylem of a eucalyptus hybrid}, volume={169}, ISSN={["1943-2631"]}, DOI={10.1534/genetics.104.039198}, abstractNote={Abstract}, number={4}, journal={GENETICS}, author={Kirst, M and Basten, CJ and Myburg, AA and Zeng, ZB and Sederoff, RR}, year={2005}, month={Apr}, pages={2295–2303} } @article{kirst_myburg_de leon_kirst_scott_sederoff_2004, title={Coordinated genetic regulation of growth and lignin revealed by quantitative trait locus analysis of cDNA microarray data in an interspecific backcross of eucalyptus}, volume={135}, ISSN={["1532-2548"]}, DOI={10.1104/pp.103.037960}, abstractNote={Abstract}, number={4}, journal={PLANT PHYSIOLOGY}, author={Kirst, M and Myburg, AA and De Leon, JPG and Kirst, ME and Scott, J and Sederoff, R}, year={2004}, month={Aug}, pages={2368–2378} } @article{myburg_griffin_sederoff_whetten_2003, title={Comparative genetic linkage maps of Eucalyptus grandis, Eucalyptus globulus and their F1 hybrid based on a double pseudo-backcross mapping approach}, volume={107}, ISSN={0040-5752 1432-2242}, url={http://dx.doi.org/10.1007/s00122-003-1347-4}, DOI={10.1007/s00122-003-1347-4}, abstractNote={Comparative genetic mapping in interspecific pedigrees presents a powerful approach to study genetic differentiation, genome evolution and reproductive isolation in diverging species. We used this approach for genetic analysis of an F(1) hybrid of two Eucalyptus tree species, Eucalyptus grandis (W. Hill ex Maiden.) and Eucalyptus globulus (Labill.). This wide interspecific cross is characterized by hybrid inviability and hybrid abnormality. Approximately 20% of loci in the genome of the F(1) hybrid are expected to be hemizygous due to a difference in genome size between E. grandis (640 Mbp) and E. globulus (530 Mbp). We investigated the extent of colinearity between the two genomes and the distribution of hemizygous loci in the F(1) hybrid using high-throughput, semi-automated AFLP marker analysis. Two pseudo-backcross families (backcrosses of an F(1) individual to non-parental individuals of the parental species) were each genotyped with more than 800 AFLP markers. This allowed construction of de novo comparative genetic linkage maps of the F(1) hybrid and the two backcross parents. All shared AFLP marker loci in the three single-tree parental maps were found to be colinear and little evidence was found for gross chromosomal rearrangements. Our results suggest that hemizygous AFLP loci are dispersed throughout the E. grandis chromosomes of the F(1) hybrid.}, number={6}, journal={Theoretical and Applied Genetics}, publisher={Springer Science and Business Media LLC}, author={Myburg, A. A. and Griffin, A. R. and Sederoff, R. R. and Whetten, R. W.}, year={2003}, month={Jul}, pages={1028–1042} } @article{myburg_remington_dm o'malley_sederoff_whetten_2001, title={High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer}, volume={30}, ISSN={["0736-6205"]}, DOI={10.2144/01302tt04}, abstractNote={ Amplified fragment length polymorphism (AFLP) analysis is currently the most powerful and efficient technique for the generation of large numbers of anonymous DNA markers in plant and animal genomes. We have developed a protocol for high-throughput AFLP analysis that allows up to 70 000 polymorphic marker genotype determinations per week on a single automated DNA sequencer. This throughput is based on multiplexed PCR amplification of AFLP fragments using two different infrared dyelabeled primer combinations. The multiplexed AFLPs are resolved on a two-dye, model 4200 LI-COR® automated DNA sequencer, and the digital images are scored using semi-automated scoring software specifically designed for complex AFLP banding patterns (AFLP-Quantar™). Throughput is enhanced by using high-quality genomic DNA templates obtained by a 96-well DNA isolation procedure. }, number={2}, journal={BIOTECHNIQUES}, author={Myburg, AA and Remington, DL and DM O'Malley and Sederoff, RR and Whetten, RW}, year={2001}, month={Feb}, pages={348-+} } @article{myburg_cawood_wingfield_botha_1998, title={Development of RAPD and SCAR markers linked to the Russian wheat aphid resistance gene Dn2 in wheat}, volume={96}, ISSN={["0040-5752"]}, DOI={10.1007/s001220050852}, number={8}, journal={THEORETICAL AND APPLIED GENETICS}, author={Myburg, AA and Cawood, M and Wingfield, BD and Botha, AM}, year={1998}, month={Jun}, pages={1162–1169} } @article{myburg_botha_wingfield_wilding_1997, title={Identification and genetic distance analysis of wheat cultivars using RAPD fingerprinting}, volume={25}, number={4}, journal={Cereal Research Communications}, author={Myburg, A. A. and Botha, A. M. and Wingfield, B. D. and Wilding, W. J. M}, year={1997}, pages={875–882} }