@article{gemenet_kitavi_david_ndege_ssali_swanckaert_makunde_yencho_gruneberg_carey_et al._2020, title={Development of diagnostic SNP markers for quality assurance and control in sweetpotato [Ipomoea batatas (L.) Lam.] breeding programs}, volume={15}, ISSN={1932-6203}, url={http://dx.doi.org/10.1371/journal.pone.0232173}, DOI={10.1371/journal.pone.0232173}, abstractNote={Quality assurance and control (QA/QC) is an essential element of a breeding program’s optimization efforts towards increased genetic gains. Due to auto-hexaploid genome complexity, a low-cost marker platform for routine QA/QC in sweetpotato breeding programs is still unavailable. We used 662 parents of the International Potato Center (CIP)’s global breeding program spanning Peru, Uganda, Mozambique and Ghana, to develop a low-density highly informative single nucleotide polymorphism (SNP) marker set to be deployed for routine QA/QC. Segregation of the selected 30 SNPs (two SNPs per base chromosome) in a recombined breeding population was evaluated using 282 progeny from some of the parents above. The progeny were replicated from in-vitro, screenhouse and field, and the selected SNP-set was confirmed to identify relatively similar mislabeling error rates as a high density SNP-set of 10,159 markers. Six additional trait-specific markers were added to the selected SNP set from previous quantitative trait loci mapping studies. The 36-SNP set will be deployed for QA/QC in breeding pipelines and in fingerprinting of advanced clones or released varieties to monitor genetic gains in famers’ fields. The study also enabled evaluation of CIP’s global breeding population structure and the effect of some of the most devastating stresses like sweetpotato virus disease on genetic variation management. These results will inform future deployment of genomic selection in sweetpotato.}, number={4}, journal={PLOS ONE}, publisher={Public Library of Science (PLoS)}, author={Gemenet, Dorcus C. and Kitavi, Mercy N. and David, Maria and Ndege, Dorcah and Ssali, Reuben T. and Swanckaert, Jolien and Makunde, Godwill and Yencho, G. Craig and Gruneberg, Wolfgang and Carey, Edward and et al.}, editor={Kumar, AjayEditor}, year={2020}, month={Apr}, pages={e0232173} }
 @article{mwanga_yencho_gibson_moyer_2013, title={Methodology for Inoculating Sweetpotato Virus Disease: Discovery of Tip Dieback, and Plant Recovery and Reversion in Different Clones}, volume={97}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-12-11-1072-re}, abstractNote={Evaluating sweetpotato (Ipomoea batatas) genotypes for resistance to sweetpotato virus disease (SPVD) has been slow and inefficient. Ipomoea setosa plants, normally used as the source of scions for graft-infecting sweetpotatoes with viral diseases, are often severely stunted and their mortality is 10 to 30% when infected with SPVD, making them unsuitable as scions. Tanzania, a landrace of I. batatas widely grown in East Africa, was found to be a superior host for maintaining and increasing SPVD inoculum (scions) for mass grafting. Modifications to a cleft-grafting technique also increased survival of grafted SPVD-affected scions from 5 to 100%. These modifications, coupled with an efficient SPVD scoring technique, allowed rapid screening of large sweetpotato populations for SPVD resistance. Plant recovery from SPVD is reported here as a component of SPVD resistance. Differences in recovery from SPVD were detected among progenies, indicating its genetic basis. Plant tip dieback, a hypersensitivity response, was observed only in families with cv. Wagabolige as a parent. These findings may open up new opportunities for improved understanding and control of this devastating disease.}, number={1}, journal={PLANT DISEASE}, publisher={Scientific Societies}, author={Mwanga, R. O. M. and Yencho, G. C. and Gibson, R. W. and Moyer, J. W.}, year={2013}, month={Jan}, pages={30–36} }
 @article{cervantes-flores_sosinski_pecota_mwanga_catignani_truong_watkins_ulmer_yencho_2011, title={Identification of quantitative trait loci for dry-matter, starch, and beta-carotene content in sweetpotato}, volume={28}, ISSN={["1380-3743"]}, DOI={10.1007/s11032-010-9474-5}, number={2}, journal={MOLECULAR BREEDING}, publisher={Springer Nature}, author={Cervantes-Flores, J. C. and Sosinski, B. and Pecota, K. V. and Mwanga, R. O. M. and Catignani, G. L. and Truong, V. D. and Watkins, R. H. and Ulmer, M. R. and Yencho, G. C.}, year={2011}, month={Aug}, pages={201–216} }
 @article{cervantes-flores_yencho_pecota_sosinski_mwanga_2008, title={Detection of quantitative trait loci and inheritance of root-knot nematode resistance in sweetpotato}, volume={133}, number={6}, journal={Journal of the American Society for Horticultural Science}, author={Cervantes-Flores, J. C. and Yencho, G. C. and Pecota, K. V. and Sosinski, B. and Mwanga, R. O. M.}, year={2008}, pages={844–851} }
 @article{cervantes-flores_yencho_kriegner_pecota_faulk_mwanga_sosinski_2008, title={Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers}, volume={21}, ISSN={["1380-3743"]}, DOI={10.1007/s11032-007-9150-6}, number={4}, journal={MOLECULAR BREEDING}, publisher={Springer Nature}, author={Cervantes-Flores, Jim C. and Yencho, G. Craig and Kriegner, Albert and Pecota, Kenneth V. and Faulk, Maria A. and Mwanga, Robert O. M. and Sosinski, Bryon R.}, year={2008}, month={May}, pages={511–532} }
 @article{mwanga_odongo_turyamureeba_alajo_yencho_gibson_smit_carey_2003, title={Release of six sweetpotato cultivars ('NASPOT 1' to 'NASPOT 6') in Uganda}, volume={38}, number={3}, journal={HortScience}, author={Mwanga, R. O. M. and Odongo, B. and Turyamureeba, G. and Alajo, A. and Yencho, G. C. and Gibson, R. W. and Smit, N. and Carey, E. E.}, year={2003}, pages={475–476} }
 @article{mwanga_yencho_moyer_2002, title={Diallel analysis of sweetpotatoes for resistance to sweetpotato virus disease}, volume={128}, ISSN={["0014-2336"]}, DOI={10.1023/A:1020828421757}, number={2}, journal={EUPHYTICA}, author={Mwanga, ROM and Yencho, CGC and Moyer, JW}, year={2002}, pages={237–248} }
 @article{mwanga_kriegner_cervantes-flores_zhang_moyer_yencho_2002, title={Resistance to sweetpotato chlorotic stunt virus and sweetpotato feathery mottle virus is mediated by two separate recessive genes in sweetpotato}, volume={127}, number={5}, journal={Journal of the American Society for Horticultural Science}, author={Mwanga, R. O. M. and Kriegner, A. and Cervantes-Flores, J. C. and Zhang, D. P. and Moyer, J. W. and Yencho, G. C.}, year={2002}, pages={798–806} }
 @article{release of five sweetpotato cultivars in uganda_2001, volume={36}, number={2}, journal={HortScience}, year={2001}, pages={385–386} }
 @article{gibson_mpembe_alicai_carey_mwanga_seal_vetten_1998, title={Symptoms, aetiology and serological analysis of sweet potato virus disease in Uganda}, volume={47}, ISSN={["0032-0862"]}, DOI={10.1046/j.1365-3059.1998.00196.x}, abstractNote={Sweet potato virus disease (SPVD) is the name used to describe a range of severe symptoms in different cultivars of sweet potato, comprising overall plant stunting combined with leaf narrowing and distortion, and chlorosis, mosaic or vein‐clearing. Affected plants of various cultivars were collected from several regions of Uganda. All samples contained the aphid‐borne sweet potato feathery mottle potyvirus (SPFMV) and almost all contained the whitefly‐borne sweet potato chlorotic stunt closterovirus (SPCSV). SPCSV was detected by a mix of monoclonal antibodies (MAb) previously shown to react only to a Kenyan isolate of SPCSV, but not by a mixture of MAb that detected SPCSV isolates from Nigeria and other countries. Sweet potato chlorotic fleck virus (SPCFV) and sweet potato mild mottle ipomovirus (SPMMV) were seldom detected in SPVD‐affected plants, while sweet potato latent virus (SPLV) was never detected. Isolates of SPFMV and SPCSV obtained by insect transmissions together induced typical symptoms of SPVD when graft‐inoculated to virus‐free sweet potato. SPCSV alone caused stunting and either purpling or yellowing of middle and lower leaves when graft‐inoculated to virus‐free plants of two cultivars. Similarly diseased naturally inoculated field plants were shown consistently to contain SPCSV. Both this disease and SPVD spread rapidly in a sweet potato crop.}, number={1}, journal={PLANT PATHOLOGY}, author={Gibson, RW and Mpembe, I and Alicai, T and Carey, EE and Mwanga, ROM and Seal, SE and Vetten, HJ}, year={1998}, month={Feb}, pages={95–102} }
 @article{gibson_mwanga_kasule_mpembe_carey_1997, title={Apparent absence of viruses in most symptomless field-grown sweet potato in Uganda}, volume={130}, ISSN={["0003-4746"]}, DOI={10.1111/j.1744-7348.1997.tb07676.x}, abstractNote={SummarySymptomless sweet potato (Ipomoea batatas) plants obtained from farmers' fields in each of the main sweet potato growing regions of Uganda were tested by nitro‐cellulose membrane enzyme‐linked immunosorbent assays (NCM‐ELISA) for sweet potato feathery mottle virus (SPFMV), sweet potato mild mottle virus (SPMMV), sweet potato chlorotic fleck virus (SPCFV), sweet potato latent virus (SPLV), C‐6 virus (C‐6) and sweet potato caulimo‐like virus (SPCa‐LV). Most of the plants tested gave negative reactions in all NCM‐ELISA, only c. 5% giving positive reactions to SPFMV and c 1% to SPMMV or SPCFV. Graft inoculations of cuttings from symptomless sweet potato plants, obtained either at Namulonge Agricultural and Animal Production Research Institute (NAARI) or from farmers' fields, onto seedlings of the near‐universal indicator plant for sweet potato viruses, Zpomoea setosa, caused symptoms in c. 15% of indicator plants; in all such cases, SPFMV alone was identified by NCM‐ELISA. Supplementary tests, involving further grafting of sweet potato to I. setosa seedlings or grafting sweet potato plants themselves with sweet potato sunken vein virus (SPSVV), which in the presence of SPFMV causes sweet potato virus disease (SPVD), confirmed the absence of infection in sweet potato cuttings which had not caused symptoms on I. setosa in the initial graft test. Possible mechanisms explaining the prevalence of virus‐free sweet potato plants in farmers' fields are discussed.}, number={3}, journal={ANNALS OF APPLIED BIOLOGY}, author={Gibson, RW and Mwanga, ROM and Kasule, S and Mpembe, I and Carey, EE}, year={1997}, month={Jun}, pages={481–490} }