@article{bodle_hamouda_cai_williams_bernacki_loboa_2019, title={Primary Cilia Exhibit Mechanosensitivity to Cyclic Tensile Strain and Lineage-Dependent Expression in Adipose-Derived Stem Cells}, volume={9}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-019-43351-y}, abstractNote={Abstract}, journal={SCIENTIFIC REPORTS}, author={Bodle, Josephine and Hamouda, Mehdi S. and Cai, Shaobo and Williams, Ramey B. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2019}, month={May} } @article{wall_dyment_bodle_volmer_loboa_cederlund_fox_banes_2016, title={Cell signaling in tenocytes: Response to load and ligands in health and disease}, volume={920}, journal={Metabolic influences on risk for tendon disorders}, author={Wall, M. E. and Dyment, N. A. and Bodle, J. and Volmer, J. and Loboa, E. and Cederlund, A. and Fox, A. M. and Banes, A. J.}, year={2016}, pages={79–95} } @misc{bodle_loboa_2016, title={Concise Review: Primary Cilia: Control Centers for Stem Cell Lineage Specification and Potential Targets for Cell-Based Therapies}, volume={34}, ISSN={["1549-4918"]}, DOI={10.1002/stem.2341}, abstractNote={Abstract}, number={6}, journal={STEM CELLS}, author={Bodle, Josephine C. and Loboa, Elizabeth G.}, year={2016}, month={Jun}, pages={1445–1454} } @article{johnson_macpherson_smith_block_keyton_2016, title={Facilitating Teamwork in Adolescent and Young Adult Oncology}, volume={12}, ISSN={["1935-469X"]}, DOI={10.1200/jop.2016.013870}, abstractNote={ A case of a young adult patient in the days immediately after a cancer diagnosis illustrates the critical importance of three interrelated core coordinating mechanisms—closed-loop communication, shared mental models, and mutual trust—of teamwork in an adolescent and young adult multidisciplinary oncology team. The case illustrates both the opportunities to increase team member coordination and the problems that can occur when coordination breaks down. A model for teamwork is presented, which highlights the relationships among these coordinating mechanisms and demonstrates how balance among them works to optimize team function and patient care. Implications for clinical practice and research suggested by the case are presented. }, number={11}, journal={JOURNAL OF ONCOLOGY PRACTICE}, author={Johnson, Rebecca H. and Macpherson, Catherine Fiona and Smith, Ashley W. and Block, Rebecca G. and Keyton, Joann}, year={2016}, month={Nov}, pages={1067-+} } @article{bodle_teeter_hluck_hardin_bernacki_loboa_2014, title={Age-Related Effects on the Potency of Human Adipose-Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications}, volume={20}, ISSN={1937-3384 1937-3392}, url={http://dx.doi.org/10.1089/ten.TEC.2013.0683}, DOI={10.1089/ten.tec.2013.0683}, abstractNote={Human adipose-derived stem cells (hASC) are now a prevalent source of adult stem cells for studies in tissue engineering and regenerative medicine. However, researchers utilizing hASC in their investigations often encounter high levels of donor-to-donor variability in hASC differentiation potential. Because of this, conducting studies with this primary cell type can require extensive resources to generate statistically significant data. We present a method to generate pooled donor cell populations, termed "superlots," containing cell populations derived from four to five age-clustered donors. The goal of generating these superlots was to 1) increase experimental throughput, 2) to utilize assay resources more efficiently, and 3) to begin to establish global hASC differentiation behaviors that may be associated with donor age. With our superlot approach, we have validated that pooled donor cell populations exhibit proliferative activity representing the combined behavior of each individual donor cell line. Further, the superlots also exhibit differentiation levels roughly approximating the average combined differentiation levels of each individual donor cell line. We established that high donor-to-donor variability exists between the pre-, peri-, and postmenopausal age groupings and that proliferation and differentiation characteristics can vary widely, independent of age. Interestingly, we did observe that cell lines derived from postmenopausal donors demonstrated a relatively high proclivity for osteogenic differentiation and a relatively lowered proclivity for adipogenic differentiation as compared with cells derived from pre- and perimenopausal donors. In general, superlots effectively represented the average differentiation behavior of each of their contributing cell populations and could provide a powerful tool for increasing experimental throughput to more efficiently utilize resources when studying hASC differentiation.}, number={12}, journal={Tissue Engineering Part C: Methods}, publisher={Mary Ann Liebert Inc}, author={Bodle, Josephine C. and Teeter, Stephanie D. and Hluck, Brandon H. and Hardin, Joseph W. and Bernacki, Susan H. and Loboa, Elizabeth G.}, year={2014}, month={Dec}, pages={972–983} } @article{mathieu_bodle_loboa_2014, title={Primary cilium mechanotransduction of tensile strain in 3D culture: Finite element analyses of strain amplification caused by tensile strain applied to a primary cilium embedded in a collagen matrix}, volume={47}, ISSN={["1873-2380"]}, DOI={10.1016/j.jbiomech.2014.04.004}, abstractNote={Human adipose-derived stem cells (hASC) exhibit multilineage differentiation potential with lineage specification that is dictated by both the chemical and mechanical stimuli to which they are exposed. We have previously shown that 10% cyclic tensile strain increases hASC osteogenesis and cell-mediated calcium accretion. We have also recently shown that primary cilia are present on hASC and that chemically-induced lineage specification of hASC concurrently results in length and conformation changes of the primary cilia. Further, we have observed cilia length changes in hASC cultured within a collagen I gel in response to 10% cyclic tensile strain. We therefore hypothesize that primary cilia may play a key mechanotransduction role for hASC exposed to tensile strain. The goal of this study was to use finite element analysis (FEA) to determine strains occurring within the ciliary membrane in response to 10% tensile strain applied parallel, or perpendicular, to cilia orientation. To elucidate the mechanical environment experienced by the cilium, several lengths were modeled and evaluated based on cilia lengths measured on hASC grown under varied culture conditions. Principal tensile strains in both hASC and ciliary membranes were calculated using FEA, and the magnitude and location of maximum principal tensile strain determined. We found that maximum principal tensile strain was concentrated at the base of the cilium. In the linear elastic model, applying strain perpendicular to the cilium resulted in maximum strains within the ciliary membrane from 150% to 200%, while applying strain parallel to the cilium resulted in much higher strains, approximately 400%. In the hyperelastic model, applying strain perpendicular to the cilium resulted in maximum strains within the ciliary membrane around 30%, while applying strain parallel to the cilium resulted in much higher strains ranging from 50% to 70%. Interestingly, FEA results indicated that primary cilium length was not directly related to ciliary membrane strain. Rather, it appears that cilium orientation may be more important than cilium length in determining sensitivity of hASC to tensile strain. This is the first study to model the effects of tensile strain on the primary cilium and provides newfound insight into the potential role of the primary cilium as a mechanosensor, particularly in tensile strain and potentially a multitude of other mechanical stimuli beyond fluid shear.}, number={9}, journal={JOURNAL OF BIOMECHANICS}, author={Mathieu, Pattie S. and Bodle, Josephine C. and Loboa, Elizabeth G.}, year={2014}, month={Jun}, pages={2211–2217} } @article{bodle_hanson_loboa_2011, title={Adipose-Derived Stem Cells in Functional Bone Tissue Engineering: Lessons from Bone Mechanobiology}, volume={17}, ISSN={["1937-3376"]}, DOI={10.1089/ten.teb.2010.0738}, abstractNote={This review aims to highlight the current and significant work in the use of adipose-derived stem cells (ASC) in functional bone tissue engineering framed through the bone mechanobiology perspective. Over a century of work on the principles of bone mechanosensitivity is now being applied to our understanding of bone development. We are just beginning to harness that potential using stem cells in bone tissue engineering. ASC are the primary focus of this review due to their abundance and relative ease of accessibility for autologous procedures. This article outlines the current knowledge base in bone mechanobiology to investigate how the knowledge from this area has been applied to the various stem cell-based approaches to engineering bone tissue constructs. Specific emphasis is placed on the use of human ASC for this application.}, number={3}, journal={TISSUE ENGINEERING PART B-REVIEWS}, author={Bodle, Josephine C. and Hanson, Ariel D. and Loboa, Elizabeth G.}, year={2011}, month={Jun}, pages={195–211} }