Sherrice Vallette Allen

Works (2)

Updated: July 5th, 2023 16:02

2005 journal article

In vitro selection of phage RB69 RegA RNA binding sites yields UAA repeats

VIROLOGY, 336(1), 26–36.

By: T. Dean n, S. Allen n & E. Miller n

author keywords: RNA SELEX; translation repressor; T4-type phage
MeSH headings : Binding Sites / genetics; Codon, Terminator / genetics; Directed Molecular Evolution; Electrophoretic Mobility Shift Assay; Genes, Viral / genetics; Protein Binding; RNA, Viral / genetics; RNA, Viral / metabolism; RNA-Binding Proteins / metabolism; Repetitive Sequences, Nucleic Acid / genetics; Repressor Proteins / metabolism; Viral Proteins / metabolism
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

RNA-binding properties of in vitro expressed histidine-tagged RB69 RegA translational repressor protein


By: S. Allen n & E. Miller n

MeSH headings : Bacteriophage T4 / genetics; Chromatography, Affinity / methods; Escherichia coli / metabolism; Histidine / genetics; Nickel / metabolism; Polymerase Chain Reaction / methods; Protein Biosynthesis; RNA / metabolism; RNA-Binding Proteins / biosynthesis; RNA-Binding Proteins / genetics; RNA-Binding Proteins / metabolism; Recombinant Proteins / metabolism; Repressor Proteins / biosynthesis; Repressor Proteins / genetics; Repressor Proteins / metabolism; Templates, Genetic; Viral Proteins / biosynthesis; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: In vitro synthesis of His-tagged proteins directly from linear PCR products provides a convenient and efficient system to study RegA and other interesting RNA-binding proteins. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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