Works (5)
Updated: July 7th, 2023 21:18
2012 journal article
Avidity-mediated virus separation using a hyperthermophilic affinity ligand
BIOTECHNOLOGY PROGRESS, 29(1), 237–246.
Contributors: n, D. Lockney n, R. Wang n, N. Gera n & B. Rao n
author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Ligands; Molecular Weight; Plant Extracts; Plant Leaves / virology; Sulfolobus solfataricus / chemistry; Sulfolobus solfataricus / metabolism; Tobacco / virology; Tombusviridae / isolation & purification
TL;DR:
The approach may be broadly relevant to cases where capture of large multivalent species from complex mixtures and subsequent release without the use of harsh elution conditions is necessary, and bypasses chemical conjugation of the affinity ligand to the capture surface.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2012 review
Protein selection using yeast surface display
[Review of ]. METHODS, 60(1), 15–26.
author keywords: Yeast surface display; Protein engineering; Library screening; Biophysical characterization; Alternative scaffolds
MeSH headings : Combinatorial Chemistry Techniques; Flow Cytometry; Protein Engineering / methods; Yeasts / genetics; Yeasts / metabolism
TL;DR:
This review presents detailed protocols for the use of yeast surface display to isolate de novo binding proteins from combinatorial libraries, and subsequent biophysical characterization of binders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2012 journal article
Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins
ACS SYNTHETIC BIOLOGY, 2(1), 6–13.
author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; alternate scaffold; mRNA display
MeSH headings : Biotechnology / methods; Carrier Proteins / genetics; Carrier Proteins / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Models, Molecular; Peptide Library; Protein Conformation; Yeasts / genetics; Yeasts / metabolism
TL;DR:
Scaffold diversification, i.e., randomization of multiple different scaffolds, is a powerful alternate strategy for combinatorial library construction and binders from this modestly sized superlibrary have affinities comparable or higher than those obtained from a library with 1000-fold higher sequence diversity but derived from a single stable scaffold.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2012 journal article
mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands
Biotechnology and Bioengineering, 110(3), 857–870.
Contributors: S. Menegatti n , n, A. Naik n, R. Carbonell n & B. Rao n
author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
MeSH headings : Humans; Immunoglobulin Fc Fragments / metabolism; Peptide Library; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / metabolism; Protein Binding; Protein Engineering / methods; RNA, Messenger / genetics; Solid-Phase Synthesis Techniques / methods
TL;DR:
The discovery, synthesis and characterization of novel cyclic peptide affinity ligands that bind the Fc portion of human Immunoglobulin G (IgG; hFc) are described and the approach can be extended to efficiently generate and evaluatecyclic peptides for other targets of interest.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2011 journal article
Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold
JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616.
Contributors: N. Gera n, n, R. Wright n & B. Rao n
author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; stability; alternate scaffold
MeSH headings : Amino Acid Sequence; Animals; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Chickens; DNA Mutational Analysis; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Fluorescein / chemistry; Fluorescein / metabolism; Immunoglobulins / chemistry; Immunoglobulins / metabolism; Mice; Molecular Sequence Data; Muramidase / chemistry; Muramidase / metabolism; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Denaturation; Streptavidin / chemistry; Streptavidin / metabolism; Sulfolobus solfataricus / chemistry; beta Catenin / chemistry; beta Catenin / metabolism
TL;DR:
It is shown that highly stable binding proteins for a wide spectrum of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus, and that S so7d-derived binding proteins could discriminate between closely related immunoglobulins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018