@article{neelam_brown-guedira_huang_2013, title={Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr21}, volume={31}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-012-9773-0}, number={1}, journal={MOLECULAR BREEDING}, author={Neelam, Kumari and Brown-Guedira, Gina and Huang, Li}, year={2013}, month={Jan}, pages={233–237} } @article{neelam_rawat_tiwari_prasad_tripathi_randhawa_dhaliwal_2012, title={Evaluation and Identification of Wheat-Aegilops Addition Lines Controlling High Grain Iron and Zinc Concentration and Mugineic Acid Production}, volume={40}, ISSN={["1788-9170"]}, DOI={10.1556/crc.40.2012.1.7}, abstractNote={Iron and zinc deficiency affects more than half of the world population due to low inherent micronutrient content of cereals and other staple foods. The micronutrient deficiency is further aggravated by poor availability of these minerals in calcareous soils and their uptake by crop plants. Series of available wheat-Aegilops addition lines were evaluated for identification of alien chromosomes carrying genes for high grain iron and zinc concentrations and release of mugineic acid(s) facilitating micronutrient uptake under their deficient conditions. Addition lines of chromosome 2Sv, 2Uv and 7Uv of Ae. peregrina, 2Sl and 7Sl of Ae. longissima and 2U of Ae. umbellulata were found to carry genes for high grain iron whereas the group 7 chromosomes had genes for higher grain zinc. Higher release of mugineic acid (MA) under iron deficient condition was observed in addition lines of chromosome 2Sv, 2Uv, 4Uv and 7Sv of Ae. peregrina, 2Sl and 6Sl of Ae. longissima and 2U and 5U of Ae. umbellulata. Higher grain and...}, number={1}, journal={CEREAL RESEARCH COMMUNICATIONS}, author={Neelam, K. and Rawat, N. and Tiwari, V. K. and Prasad, R. and Tripathi, S. K. and Randhawa, G. S. and Dhaliwal, H. S.}, year={2012}, month={Mar}, pages={53–61} } @article{rawat_neelam_tiwari_randhawa_friebe_gill_dhaliwal_2011, title={Development and molecular characterization of wheat - Aegilops kotschyi addition and substitution lines with high grain protein, iron, and zinc}, volume={54}, ISSN={["1480-3321"]}, DOI={10.1139/g11-059}, abstractNote={Over two billion people, depending largely on staple foods, suffer from deficiencies in protein and some micronutrients such as iron and zinc. Among various approaches to overcome protein and micronutrient deficiencies, biofortification through a combination of conventional and molecular breeding methods is the most feasible, cheapest, and sustainable approach. An interspecific cross was made between the wheat cultivar ‘Chinese Spring’ and Aegilops kotschyi Boiss. accession 396, which has a threefold higher grain iron and zinc concentrations and about 33% higher protein concentration than wheat cultivars. Recurrent backcrossing and selection for the micronutrient content was performed at each generation. Thirteen derivatives with high grain iron and zinc concentrations and contents, ash and ash micronutrients, and protein were analyzed for alien introgression. Morphological markers, high molecular weight glutenin subunit profiles, anchored wheat microsatellite markers, and GISH showed that addition and substitution of homoeologous groups 1, 2, and 7 chromosomes of Ae. kotschyi possess gene(s) for high grain micronutrients. The addition of 1U/1S had high molecular weight glutenin subunits with higher molecular weight than those of wheat, and the addition of 2S in most of the derivatives also enhanced grain protein content by over 20%. Low grain protein content in a derivative with a 2S-wheat translocation, waxy leaves, and absence of the gdm148 marker strongly suggests that the gene for higher grain protein content on chromosome 2S is orthologous to the grain protein QTL on the short arm of group 2 chromosomes.}, number={11}, journal={GENOME}, author={Rawat, Nidhi and Neelam, Kumari and Tiwari, Vijay K. and Randhawa, Gursharn S. and Friebe, Bernd and Gill, Bikram S. and Dhaliwal, Harcharan S.}, year={2011}, month={Nov}, pages={943–953} }