@article{zeng_ye_kerns_tredway_martin_martin_2015, title={Molecular Characterization and Phylogenetic Relationships of Plant-Parasitic Nematodes Associated with Turfgrasses in North Carolina and South Carolina, United States}, volume={99}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-10-14-1060-re}, abstractNote={ The near-full-length 18S ribosomal DNA (rDNA) gene and internal transcribed spacer 1 region were amplified and sequenced from 52 nematode populations belonging to 28 representative species in 13 families recovered from turfgrasses in North Carolina (38 populations) and South Carolina (14 populations). This study also included 13 nematode populations from eight other plant hosts from North Carolina for comparison. Nematodes were molecularly characterized and the phylogenetic relationships were explored based on 18S rDNA sequences. Phylogenetic analysis using Bayesian inference was performed using five groups of the plant-parasitic nematode populations Tylenchids, Criconematids, Longidorids, Xiphinematids, and Trichodorids. The 65 nematode populations were clustered correspondingly within appropriate positions of 13 families, including Belonolaimidae, Caloosiidae, Criconematidae, Dolichodoridae, Hemicycliophoridae, Hoplolaimidae, Heteroderidae, Longidoridae, Meloidogynidae, Paratylenchidae, Pratylenchidae, Telotylenchidae, and Trichodoridae. This study confirms previous morphological-based identification of the plant-parasitic nematode species found in turfgrasses and provides a framework for future studies of plant-parasitic nematodes associated with turfgrasses based upon DNA sequences and phylogenetic relationships. }, number={7}, journal={PLANT DISEASE}, author={Zeng, Yongsan and Ye, Weimin and Kerns, James and Tredway, Lane and Martin, Samuel and Martin, Matt}, year={2015}, month={Jul}, pages={982–993} } @article{kimball_zuleta_martin_kenworthy_chandra_milla-lewis_2012, title={Assessment of molecular variation within 'Raleigh' St. Augustinegrass using amplified fragment length polymorphism markers}, volume={47}, DOI={10.21273/hortsci.47.7.839}, abstractNote={St. augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is a popular turfgrass in the southern United States as a result of its superior shade tolerance and relatively low input requirements. However, it is the least cold-tolerant of commonly used warm-season turfgrass species. ‘Raleigh’, released in 1980, has superior cold tolerance and is adapted and widely used in U.S. Department of Agriculture hardiness zones 8 to 9. More than 25 years after its release, ‘Raleigh’ is still the industry’s standard in terms of cold tolerance. However, the original foundation and breeder stock fields of the cultivar have been lost, placing the integrity of the cultivar at risk. The objectives of this study were to investigate whether current ‘Raleigh’ production fields across the southern United States are true to the original source. In this study, 15 amplified fragment length polymorphism (AFLP) primer combinations were used to assess levels of genetic variability among three original stocks of ‘Raleigh’ and 46 samples obtained from sod farms and universities in six states. Genetic similarities among the original stocks were Sij = 1, whereas similarities between this group and all other samples ranged from 0.24 to 1.0. Results based on cluster analysis, principal coordinate analysis, and analysis of molecular variance (AMOVA) revealed separation between original stocks of ‘Raleigh’ and some commercial samples. Results from this study offer further evidence that molecular markers provide a useful and powerful technique for identity preservation of clonally propagated cultivars and the detection of genetic variants in sod production fields and turfgrass breeding programs.}, number={7}, journal={HortScience}, publisher={American Society for Horticultural Science}, author={Kimball, J. A. and Zuleta, M. C. and Martin, M. C. and Kenworthy, K. E. and Chandra, A. and Milla-Lewis, S. R.}, year={2012}, pages={839–844} } @article{zeng_ye_martin_martin_tredway_2012, title={Diversity and occurrence of plant-parasitic nematodes associated with golf course turfgrasses in North and South Carolina, USA}, volume={44}, number={4}, journal={Journal of Nematology}, author={Zeng, Y. S. and Ye, W. M. and Martin, B. and Martin, M. and Tredway, L.}, year={2012}, pages={337–347} } @article{zeng_ye_tredway_martin_martin_2012, title={Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA}, number={3452}, journal={Zootaxa}, author={Zeng, Y. S. and Ye, W. M. and Tredway, L. and Martin, S. and Martin, M.}, year={2012}, pages={1–46} }