@article{dunne_tuong_livingston_reynolds_milla-lewis_2019, title={Field and Laboratory Evaluation of Bermudagrass Germplasm for Cold Hardiness and Freezing Tolerance}, volume={59}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2017.11.0667}, abstractNote={Bermudagrass [Cynodon spp. (L.) Rich.] is a high‐quality, durable turfgrass with excellent heat and drought tolerance. However, its lack of freezing tolerance limits its use in the transition zone. The development of cultivars with enhanced freezing tolerance would constitute a significant improvement in the management of bermudagrass in this region and could extend its area of adaptation further north. There has been substantial work on screening of common‐type bermudagrass [Cynodon dactylon (L.) Pers.] germplasm for freezing tolerance, but not for the African (Cynodon transvaalensis Burtt‐Davy) germplasm. The purpose of this research was to conduct multiyear field testing and laboratory‐based freezing test evaluations of winter hardiness and freezing tolerance, respectively, of an African and common bermudagrass germplasm collection. A high level of cold hardiness was observed among the germplasm in this study. In field evaluations, plant introductions (PIs) PI 290905, PI 647879, PI 255447, PI 289923, and PI 615161 were the top performers, having consistently greater spring green‐up and reduced winterkill compared with ‘Patriot’, ‘Tifsport’, ‘Quickstand’, and ‘Tifway’, though not always significantly. A comparison between field‐based ratings and calculated lethal temperatures for 50% death (LT50) from laboratory‐based freezing tests showed significant correlations of −0.26 and −0.24 for spring green‐up and winterkill, respectively, suggesting that these controlled freeze experiments could be used to prescreen materials prior to field testing. Overall, results indicate that some of the PIs evaluated in this study can be used as additional sources of cold hardiness in bermudagrass breeding.}, number={1}, journal={CROP SCIENCE}, publisher={Crop Science Society of America}, author={Dunne, Jeffrey C. and Tuong, Tan D. and Livingston, David P. and Reynolds, W. Casey and Milla-Lewis, Susana R.}, year={2019}, pages={392–399} }
 @article{patel_milla-lewis_zhang_templeton_reynolds_richardson_biswas_zuleta_dewey_qu_et al._2015, title={Overexpression of ubiquitin-like LpHUB1 gene confers drought tolerance in perennial ryegrass}, volume={13}, ISSN={["1467-7652"]}, DOI={10.1111/pbi.12291}, abstractNote={SummaryHUB1, also known as Ubl5, is a member of the subfamily of ubiquitin‐like post‐translational modifiers. HUB1 exerts its role by conjugating with protein targets. The function of this protein has not been studied in plants. A HUB1 gene, LpHUB1, was identified from serial analysis of gene expression data and cloned from perennial ryegrass. The expression of this gene was reported previously to be elevated in pastures during the summer and by drought stress in climate‐controlled growth chambers. Here, pasture‐type and turf‐type transgenic perennial ryegrass plants overexpressing LpHUB1 showed improved drought tolerance, as evidenced by improved turf quality, maintenance of turgor and increased growth. Additional analyses revealed that the transgenic plants generally displayed higher relative water content, leaf water potential, and chlorophyll content and increased photosynthetic rate when subjected to drought stress. These results suggest HUB1 may play an important role in the tolerance of perennial ryegrass to abiotic stresses.}, number={5}, journal={PLANT BIOTECHNOLOGY JOURNAL}, publisher={Wiley-Blackwell}, author={Patel, Minesh and Milla-Lewis, Susana and Zhang, Wanjun and Templeton, Kerry and Reynolds, William C. and Richardson, Kim and Biswas, Margaret and Zuleta, Maria C. and Dewey, Ralph E. and Qu, Rongda and et al.}, year={2015}, month={Jun}, pages={689–699} }
 @article{reynolds_miller_rufty_2013, title={Athletic Field Paint Color Differentially Alters Light Spectral Quality and Bermudagrass Photosynthesis}, volume={53}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2013.01.0014}, abstractNote={ABSTRACTPainting of athletic fields is widespread throughout the world and can often cause declines in turfgrass health. Visible light and photosynthesis share the same wavelengths (400–700 nm), and it was hypothesized that alterations in visible light to produce specific colors would lead to reductions in photosynthetically active radiation (PAR) and total canopy photosynthesis (TCP). Lab experiments using a spectroradiometer and LICOR 1800‐12 integrating sphere examined the impacts of 10 colors of athletic field paint on PAR as well as wavelengths within PAR. These colors were then applied weekly for 5 wk to ‘Tifway’ bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt Davy], and TCP was measured using a gas exchange system 24 h after each application. Spectroradiometry analyses revealed the significant effects of paint color (P ≤ 0.001) on reflection, transmission, and absorption of PAR. Lighter colors including white, yellow, orange, and red reflected 47 to 92% of PAR while darker colors including green, black, and dark blue absorbed 87 to 95% of PAR. Accompanying gas exchange measurements revealed that TCP was most negatively correlated with absorption of PAR (r = −0.959, P ≤ 0.001) and that darker colors negatively impact TCP more than lighter colors. The results clearly indicate that damage to turfgrasses with long‐term painting will be difficult to avoid, and this is particularly true with darker colors of paint.}, number={5}, journal={CROP SCIENCE}, author={Reynolds, William Casey and Miller, Grady L. and Rufty, Thomas W.}, year={2013}, pages={2209–2217} }
 @article{reynolds_miller_rufty_2012, title={Athletic Field Paint Impacts Light Spectral Quality and Turfgrass Photosynthesis}, volume={52}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2012.01.0059}, abstractNote={ABSTRACTAthletic field paints are applied to turf surfaces with little or no acute injury. However, field managers notice chronic declines in turfgrass health after repeated applications. This study examines athletic field paint impacts on spectral quality and associated turfgrass photosynthesis. Growth chamber experiments evaluated effects of red and white athletic field paint as well as one, two, three, and four repeated weekly applications on total canopy photosynthesis (TCP) of perennial ryegrass (Lolium perenne L.). Paint treatments were applied weekly for 6 wk with TCP recorded 24 h after each application using a gas exchange system. Spectroradiometry experiments evaluated reflection, absorption, and transmission of light at various wavelengths based on paint color, dilution, and thickness. Over a six week period all treatments reduced TCP based on color (P ≤ 0.0001) and dilution (P ≤ 0.0001). Red no‐dilution paint produced a 75% reduction in TCP over 6 wk while white 1:1 diluted paint only produced a 19% reduction. Spectroradiometry data suggests this is likely due to reductions in photosynthetically active radiation (PAR) with red paint absorbing 51% of incident PAR while transmitting and reflecting 6 and 43%, respectively. White paint transmitted 5% of PAR while reflecting 95%. Alterations in light spectral quality resulting from athletic field paint applications can impact PAR, which may result in reduced turfgrass health.}, number={5}, journal={CROP SCIENCE}, author={Reynolds, William Casey and Miller, Grady L. and Rufty, Thomas W.}, year={2012}, month={Sep}, pages={2375–2384} }