Maria Fernanda Rodriguez-Welsh

Works (4)

Updated: August 8th, 2023 21:20

2015 journal article

A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants

PLASMID, 81, 55–62.

By: J. Dalal  n, R. Yalamanchili n, C. Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n  ...

Contributors: J. Dalal  n, R. Yalamanchili n, C. La Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n , H. Sederoff n , R. Qu n

author keywords: Gateway; Plant transformation; Agrobacterium; Gene stacking
MeSH headings : Cloning, Molecular / methods; Gene Expression; Gene Order; Genes, Reporter; Genetic Vectors / genetics; Plants, Genetically Modified; Plasmids / genetics; Transformation, Genetic; Transgenes
TL;DR: The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 chapter

Methods for RNA Profiling of Gravi-Responding Plant Tissues

In Methods in Molecular Biology (Vol. 1309, pp. 91–117).

By: J. Dalal  n, E. Land n , N. Vasani n, L. He n, C. Smith  n, M. Rodriguez-Welsh n, I. Perera n , H. Sederoff n 

Contributors: J. Dalal  n, E. Land n , N. Vasani n, L. He n, C. Smith  n, M. Rodriguez-Welsh n, I. Perera n , H. Sederoff n 

author keywords: RNA; Transcript; Transcriptome; Sequencing; PCR; RNAseq; sRNA; miRNA
MeSH headings : Gene Expression Profiling / methods; Gene Expression Regulation, Plant; Gravity Sensing / genetics; Oligonucleotide Array Sequence Analysis / methods; RNA / genetics; RNA, Messenger / biosynthesis; RNA, Messenger / genetics; Seeds / genetics; Seeds / growth & development; Transcriptome / genetics
TL;DR: The current and most widely used methods to profile the changes in an entire transcriptome by high-throughput sequencing of RNA fractions (RNAseq) and single gene transcript analysis using real-time quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) are described. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2014 journal article

Homotypic Vacuole Fusion Requires VTI11 and Is Regulated by Phosphoinositides

MOLECULAR PLANT, 7(6), 1026–1040.

By: J. Zheng n, S. Han n, M. Rodriguez-Welsh n & M. Rojas-Pierce  n

Contributors: J. Zheng n, S. Han n, M. Rodriguez-Welsh n & M. Rojas-Pierce  n

author keywords: vacuole; SNARE; phosphoinositides; membrane fusion; Wortmannin
MeSH headings : Androstadienes / pharmacology; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Chromones / pharmacology; Morpholines / pharmacology; Phosphatidylinositol Phosphates / metabolism; Phosphoinositide-3 Kinase Inhibitors; Qb-SNARE Proteins / metabolism; Vacuoles / metabolism; Wortmannin
TL;DR: A ubiquitous role of phosphoinositides in vacuole fusion is suggested, both during the development of the large central vacuoles and during the dynamic vacUole remodeling that occurs as part of stomata movements. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

A Small Molecule Inhibitor Partitions Two Distinct Pathways for Trafficking of Tonoplast Intrinsic Proteins in Arabidopsis

PLOS ONE, 7(9).

By: E. Rivera-Serrano n, M. Rodriguez-Welsh n, G. Hicks * & M. Rojas-Pierce  n

Contributors: E. Rivera-Serrano n, M. Rodriguez-Welsh n, G. Hicks * & M. Rojas-Pierce  n

Ed(s): E. Damme

MeSH headings : Acyltransferases / genetics; Acyltransferases / physiology; Aquaporins / genetics; Aquaporins / physiology; Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Brefeldin A / pharmacology; Cell Membrane / metabolism; Golgi Apparatus / metabolism; Green Fluorescent Proteins / metabolism; Hypocotyl / metabolism; Microscopy, Confocal / methods; Models, Biological; Promoter Regions, Genetic; Protein Transport; Seedlings; Vacuoles / metabolism
TL;DR: It is shown that members of the TIP family are targeted to the vacuole via at least two distinct pathways, and the bioactivity of a novel inhibitor that can differentiate between them is characterized and mediated in part by common mechanisms. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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