@article{siminszky_gavilano_bowen_dewey_2005, title={Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase}, volume={102}, DOI={10.1073/pnas.050658102}, number={41}, journal={Proceedings of the National Academy of Sciences of the United States of America}, author={Siminszky, B. and Gavilano, L. and Bowen, S. W. and Dewey, R. E.}, year={2005}, pages={14919–14924} }
 @article{siminszky_freytag_sheldon_dewey_2003, title={Co-expression of a NADPH : P450 reductase enhances CYP71A10-dependent phenylurea metabolism in tobacco}, volume={77}, ISSN={["0048-3575"]}, DOI={10.1016/j.pestbp.2003.08.001}, abstractNote={A soybean cytochrome P450 monooxygenase, designated CYP71A10, catalyzes the oxidative N-demethylation or ring methyl hydroxylation of a variety of phenylurea herbicides. The ectopic expression of CYP71A10 in tobacco was previously shown to be an effective means of enhancing whole plant tolerance to the compounds linuron and chlortoluron. Because P450 enzymes require ancillary proteins to catalyze the transfer of electrons from NADPH to the functional heme group of the P450, it is possible that the endogenous levels of these companion proteins may be insufficient to support the optimal activation of a highly expressed recombinant P450. In the present report, we have generated transgenic tobacco that simultaneously express CYP71A10 and a soybean P450 reductase. Transformed plants that express both CYP71A10 and the P450 reductase demonstrated 20–23% higher metabolic activity against phenylurea herbicides than control plants expressing CYP71A10 alone. These results suggest that herbicide tolerance strategies based on the expression of P450 genes may require concomitant expression of a cognate electron transport partner to fully exploit the herbicide metabolic capacity of the P450.}, number={2}, journal={PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY}, author={Siminszky, B and Freytag, AM and Sheldon, BS and Dewey, RE}, year={2003}, month={Oct}, pages={35–43} }
 @article{siminszky_sheldon_corbin_dewey_2000, title={A cytochrome P450 monooxygenase cDNA (CYP71A10) confers resistance to linuron in transgenic Nicotiana tabacum}, volume={48}, ISSN={["0043-1745"]}, DOI={10.1614/0043-1745(2000)048[0291:ACPMCC]2.0.CO;2}, abstractNote={Abstract The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA designated CYP71A10 that conferred linuron resistance to laboratory-grown, transgenic Nicotiana tabacum seedlings was previously reported. A nonsegregating transgenic N. tabacum line has been established that possesses two independent copies of the G. max CYP71A10 transgene. Five-week-old progeny plants of this selected line were grown in a controlled environmental chamber and treated with linuron using either pretransplant incorporated (PTI) or postemergence (POST) applications. CYP71A10-transformed N. tabacum was more tolerant to linuron than the wild type for both application methods. The transgenic N. tabacum line tolerated an approximately 16-fold and 12-fold higher rate of linuron than wild-type N. tabacum when the herbicide was applied PTI or POST, respectively. These results provide evidence that plant-derived P450 genes can be employed effectively to confer herbicide resistance to transgenic plants. Nomenclature: Cytochrome P450; linuron; Glycine max L. Merr. ‘Dare’, soybean; Nicotiana tabacum L. ‘SR1’, tobacco.}, number={3}, journal={WEED SCIENCE}, author={Siminszky, B and Sheldon, BS and Corbin, FT and Dewey, RE}, year={2000}, pages={291–295} }
 @misc{siminszky_dewey_corbin_2000, title={Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants}, volume={6,121,512}, number={2000 Sept. 19}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Siminszky, B. and Dewey, R. and Corbin, F.}, year={2000} }
 @article{siminszky_corbin_ward_fleischmann_dewey_1999, title={Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides}, volume={96}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.96.4.1750}, abstractNote={
            A strategy based on the random isolation and screening of soybean
 cDNAs encoding cytochrome P450 monooxygenases (P450s) was used in an
 attempt to identify P450 isozymes involved in herbicide metabolism.
 Nine full-length (or near-full-length) P450 cDNAs representing eight
 distinct P450 families were isolated by using PCR-based technologies.
 Five of the soybean P450 cDNAs were expressed successfully in yeast,
 and microsomal fractions generated from these strains were tested for
 their potential to catalyze the metabolism of 10 herbicides and 1
 insecticide.
            In vitro
            enzyme assays showed that the gene
 product of one heterologously expressed P450 cDNA (CYP71A10)
 specifically catalyzed the metabolism of phenylurea herbicides,
 converting four herbicides of this class (fluometuron, linuron,
 chlortoluron, and diuron) into more polar compounds. Analyses of the
 metabolites suggest that the CYP71A10 encoded enzyme functions
 primarily as an
            N
            -demethylase with regard to
 fluometuron, linuron, and diuron, and as a ring-methyl hydroxylase when
 chlortoluron is the substrate.
            In vivo
            assays using
 excised leaves demonstrated that all four herbicides were more readily
 metabolized in CYP71A10-transformed tobacco compared with control
 plants. For linuron and chlortoluron, CYP71A10-mediated herbicide
 metabolism resulted in significantly enhanced tolerance to these
 compounds in the transgenic plants.
          }, number={4}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Siminszky, B and Corbin, FT and Ward, ER and Fleischmann, TJ and Dewey, RE}, year={1999}, month={Feb}, pages={1750–1755} }