@article{de souza_huang_tarpy_2019, title={Experimental improvement of honey bee (Apis mellifera) queen quality through nutritional and hormonal supplementation}, volume={50}, ISSN={["1297-9678"]}, DOI={10.1007/s13592-018-0614-y}, abstractNote={Queen reproductive potential (=quality) impacts the health and productivity of honey bee colonies. To determine the factors that affect reproductive quality during development, we tested queens produced under larval treatments by supplementing the diet with juvenile hormone (JH), additional sugars, or both, compared to untreated control. Furthermore, we varied the age of the larvae that were grafted (1 and 3 days old). We analyzed newly emerged virgin queens for their morphological characters as proxies for their reproductive potential. We found that the application of a sugar-enriched diet in combination with JH application onto 1st instar queen larvae produced higher-quality queens, while for 3rd instar larvae only the JH treatment resulted in increasing queen quality. For mated queens, those treated with JH plus supplemented sugars showed a significantly higher sperm count and sperm viability. Our findings demonstrate that honey bee queen reproductive potential can be increased through diet supplementation.}, number={1}, journal={APIDOLOGIE}, author={De Souza, Daiana A. and Huang, Ming Hua and Tarpy, David R.}, year={2019}, month={Feb}, pages={14–27} } @article{simone-finstrom_li-byarlay_huang_strand_rueppell_tarpy_2016, title={Migratory management and environmental conditions affect lifespan and oxidative stress in honey bees}, volume={6}, DOI={10.1038/srep32023}, abstractNote={AbstractMost pollination in large-scale agriculture is dependent on managed colonies of a single species, the honey bee Apis mellifera. More than 1 million hives are transported to California each year just to pollinate the almonds and bees are trucked across the country for various cropping systems. Concerns have been raised about whether such “migratory management” causes bees undue stress; however to date there have been no longer-term studies rigorously addressing whether migratory management is detrimental to bee health. To address this issue, we conducted field experiments comparing bees from commercial and experimental migratory beekeeping operations to those from stationary colonies to quantify effects on lifespan, colony health and productivity and levels of oxidative damage for individual bees. We detected a significant decrease in lifespan of migratory adult bees relative to stationary bees. We also found that migration affected oxidative stress levels in honey bees, but that food scarcity had an even larger impact; some detrimental effects of migration may be alleviated by a greater abundance of forage. In addition, rearing conditions affect levels of oxidative damage incurred as adults. This is the first comprehensive study on impacts of migratory management on the health and oxidative stress of honey bees.}, journal={Scientific Reports}, author={Simone-Finstrom, M. and Li-Byarlay, H. and Huang, M. H. and Strand, M. K. and Rueppell, O. and Tarpy, David}, year={2016} } @article{degrandi-hoffman_eckholm_huang_2013, title={Concentrations of nutrients in pollen can change after conversion to bee bread}, volume={153}, number={11}, journal={American Bee Journal}, author={DeGrandi-Hoffman, G. and Eckholm, B. and Huang, M. H.}, year={2013}, pages={1195–1198} }