@article{fruchtman_mcvey_borski_2002, title={Characterization of pituitary IGF-I receptors: modulation of prolactin and growth hormone}, volume={283}, ISSN={["1522-1490"]}, DOI={10.1152/ajpregu.00511.2001}, abstractNote={There have been no studies in any vertebrate that have localized insulin-like growth factor (IGF)-I receptors in prolactin (PRL) cells or that have correlated pituitary binding to the potency of IGF-I in regulating both PRL and growth hormone (GH) secretion. We show that IGF-I binds with high affinity and specificity to the pituitary gland of hybrid striped bass ( Morone saxatilis × M. chrysops). IGF-I and IGF-II were equipotent in inhibiting saturable125I-IGF-I binding, whereas insulin was ineffective. IGF-I binds with similar affinity to the rostral pars distalis (>95% PRL cells) as the whole pituitary gland and immunohistochemistry colocalizes IGF-I receptors and PRL in this same region. Des(1–3)IGF-I, a truncated analog of IGF-I that binds with high affinity to IGF-I receptors but weakly to IGF-I binding proteins (IGFBPs), showed a similar inhibition of saturable125I-IGF-I binding, but it was more potent than IGF-I in stimulating PRL and inhibiting GH release. These results are the first to localize IGF-I receptors to PRL cells, correlate IGF-I binding to its efficacy in regulating GH and PRL secretion, as well as demonstrate that IGFBPs may play a significant role in modulating the disparate actions of IGF-I on PRL and GH secretion.}, number={2}, journal={AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY}, author={Fruchtman, S and McVey, DC and Borski, RJ}, year={2002}, month={Aug}, pages={R468–R476} } @article{smith_mcvey_jenkins_denson_heyward_sullivan_berlinsky_1999, title={Broodstock management and spawning of southern flounder, Paralichthys lethostigma}, volume={176}, ISSN={["0044-8486"]}, DOI={10.1016/S0044-8486(99)00053-8}, abstractNote={Collaborative studies are underway in South Carolina (SC) and North Carolina (NC) to control reproduction of southern flounder, Paralichthys lethostigma. Although cultured broodstock are being developed, work to date has been with wild caught adults held in captivity for at least 1 year. Shortly after capture, wild adults should be treated to control diseases and parasites, especially Amyloodinium sp. and fish lice, Argulus sp. Induced spawning using only photothermal control has not occurred, but GnRHa implants have been successfully used to induce ovulation and allow strip-spawning. In addition, during 1997, photothermal conditioning coupled with 100 μg GnRHa implants resulted in successful tank-spawning. During a 99-day period, eggs were collected on 64 days and lowering temperature was shown to inhibit spawning. On days that spawning occurred, mean number of eggs collected was 277,844 (±177,714) and mean fertility was 32.8% (±25.2%). Total number of eggs collected was 17,782,000. Females used in the study were ≥5 years old while the males were ≥3 years old. Fish had been in captivity for ≥1.5 years. The spawning success achieved using the combination of photothermal conditioning and GnRHa implants resulted in less stress to the fish, higher egg production and an extended spawning period. Use of photothermal conditioning coupled with tank-spawning techniques should allow year-round spawning of southern flounder. This should facilitate more rapid development of a culture technology for this species.}, number={1-2}, journal={AQUACULTURE}, author={Smith, TIJ and McVey, DC and Jenkins, WE and Denson, MR and Heyward, LD and Sullivan, CV and Berlinsky, DL}, year={1999}, month={Jun}, pages={87–99} }