@article{lin_bo_oliver_corl_jacobi_oliver_harrell_odle_2011, title={Dietary conjugated linoleic acid alters long chain polyunsaturated fatty acid metabolism in brain and liver of neonatal pigs}, volume={22}, ISSN={0955-2863}, url={http://dx.doi.org/10.1016/j.jnutbio.2010.09.002}, DOI={10.1016/j.jnutbio.2010.09.002}, abstractNote={Effects of dietary conjugated linoleic acid (CLA, 1% mixed isomers) on n-6 long-chain polyunsaturated fatty acid (LCPUFA) oxidation and biosynthesis were investigated in liver and brain tissues of neonatal piglets. Fatty acid β-oxidation was measured in tissue homogenates using [1-14C]linoleic acid (LA) and -arachidonic acid (ARA) substrates, while fatty acid desaturation and elongation were traced using [U-13C]LA and GC-MS. Dietary CLA had no effect on fatty acid β-oxidation, but significantly decreased n-6 LCPUFA biosynthesis by inhibition of LA elongation and desaturation. Differences were noted between our 13C tracer assessment of desaturation/elongation and simple precursor-product indices computed from fatty acid composition data, indicating that caution should be exercised when employing the later. The inhibitory effects of CLA on elongation/desaturation were more pronounced in pigs fed a low fat diet (3% fat) than a high fat diet (25% fat). Direct elongation of linoleic acid to C20:2n-6 via the alternate elongation pathway might play an important role in n-6 LCPUFA synthesis because more than 40% of the synthetic products of [U-13C]LA accumulated in [13C]20:2n-6. Overall, the data show that dietary CLA shifted the distribution of the synthetic products of [U-13C]LA between elongation and desaturation in liver and decreased the total synthetic products of [U-13C]LA in brain by inhibiting LA elongation to C20:2n-6. The impact of CLA on brain LCPUFA metabolism of the developing neonate merits consideration and further investigation.}, number={11}, journal={The Journal of Nutritional Biochemistry}, publisher={Elsevier BV}, author={Lin, Xi and Bo, Jenny and Oliver, Susan A. Mathews and Corl, Benjamin A. and Jacobi, Sheila K. and Oliver, William T. and Harrell, Robert J. and Odle, Jack}, year={2011}, month={Nov}, pages={1047–1054} }
 @article{oliver_touchette_coalson_whisnant_brown_oliver_odle_harrell_2005, title={Pigs weaned from the sow at 10 days of age respond to dietary energy source of manufactured liquid diets and exogenous porcine somatotropin}, volume={83}, DOI={10.2527/2005.8351002x}, abstractNote={Previous research indicates that the neonatal pig does not alter feed intake in response to changes in the energy density of manufactured liquid diets. Also, the limited response of IGF-I to exogenous porcine ST (pST) previously observed in young pigs may be influenced by the source of dietary energy. Our objectives were to 1) determine the effect of a high-fat (HF; 25% fat and 4,639 kcal/kg ME; DM basis) or low-fat (LF; 2% fat and 3,481 kcal/kg ME; DM basis) manufactured liquid diet on pig performance; and 2) determine whether the limited response to exogenous pST in young pigs depends on the source of dietary energy. Two replicates of 60 pigs (n = 120; barrows and gilts distributed evenly), with an initial BW of 4,207 +/- 51 g, were weaned from the sow at 10 d of age and used in a randomized complete block design. Pigs were assigned by BW to one of six pens. Diets were formulated to provide a constant lysine:ME ratio and were fed on a pen basis for a duration of 9 d. On d 5, barrows and gilts within a pen were assigned randomly to receive either 0 or 120 microg of pST.kg BW(-1).d(-1) for 4 d. Pigs gained 336 +/- 9 g/d, which resulted in an ending BW of 7,228 +/- 120 g, regardless of dietary treatment (P > 0.15). Pigs fed the LF diet consumed 17% more DM per pen daily than pigs fed the HF diet (2,777 +/- 67 vs. 2,376 +/- 67 g/d, P < 0.01), but calculated ME intake did not differ between dietary treatments (P > 0.20). The G:F was 24% greater in HF- than in LF-fed pigs (P < 0.01). Plasma urea N concentrations were higher in the HF-fed pigs (11.0 +/- 0.6 mg/dL) than in pigs fed the LF diet (6.2 +/- 0.6 mg/dL; P < 0.05). Treatment with pST increased circulating IGF-I (P < 0.01) and decreased PUN (P < 0.01) concentration 32 and 25%, respectively, regardless of dietary treatment (P > 0.30). Circulating leptin averaged 1.8 +/- 0.1 ng/mL and was not affected by dietary treatment (P > 0.35) or pST (P > 0.40). These results suggest that the ST/IGF axis is responsive in the young pig and the increase in circulating IGF-I and growth is independent of the source of dietary energy. Also, young pigs respond to a lower energy density liquid diet with increased feed intake, without altering growth performance, apparently utilizing a mechanism other than circulating leptin.}, number={5}, journal={Journal of Animal Science}, author={Oliver, W. T. and Touchette, K. J. and Coalson, J. A. and Whisnant, C. S. and Brown, J. A. and Oliver, S. A. M. and Odle, J. and Harrell, R. J.}, year={2005}, pages={1002–1009} }
 @article{oliver_mccauley_harrell_sustert_kerton_dunshea_2003, title={A  gonadotropin-releasing factor vaccine (Improvac) and porcine somatotropin have synergistic and additive effects on growth performance in group-housed boars and gilts}, volume={81}, DOI={10.2527/2003.8181959x}, abstractNote={Two hundred and twenty-four pigs (112 boars, 112 gilts) housed in pens of seven pigs per pen were used in a 2 x 2 x 2 factorial design, with the factors of vaccination with a gonadotropin-releasing factor (GnRF) vaccine (Improvac; 0 or 2 mL at 13 and 17 wk of age), porcine somatotropin (pST; 0 or 5 mg/d from 17 wk of age), and gender. Pigs were weighed and feed intake was measured from 17 wk of age until slaughter at 21 wk of age. Body composition was estimated by dual-energy X-ray absorptiometry in two focus pigs per pen at 17 and 21 wk of age. Testes and ovary weights at slaughter were decreased by Improvac treatment (P < 0.001), but were not altered by pST treatment (P > 0.44). Daily gain was lower for gilts than boars (1,128 vs. 1,299 g/d, P < 0.001) and was increased by pST (1,172 vs. 1,255 g/d, P = 0.003) and Improvac (1,150 vs. 1,276 g/d, P < 0.001) treatments. Feed intake (as-fed basis) was lower in gilts than in boars (2,774 vs. 3,033 g/d, P = 0.002), was decreased by pST (3,037 vs. 2,770 g/ d, P = 0.002), and was increased by Improvac treatment (2,702 vs. 3,105 g/d, P < 0.001). As a result of the differences in feed intake and daily gain, feed conversion efficiency (gain:feed) was lower for gilts than for boars (0.403 vs. 0.427 P = 0.025), was improved by pST (0.385 vs. 0.452, P < 0.001), but was unchanged by Improvac treatment (0.423 vs. 0.410, P = 0.22). Carcass weight was lower in gilts than in boars (75.3 vs. 77.0 kg, P = 0.012), was unchanged by pST treatment (75.9 vs. 76.4 kg, P = 0.40), and was increased by Improvac treatment (75.1 vs. 77.2 kg, P = 0.003). Lean tissue deposition rate was lower in gilts than in boars (579 vs. 725 g/d, P < 0.001), was increased by pST (609 vs. 696 g/d, P < 0.001) and by Improvac treatment (623 vs. 682 g/d, P = 0.014). Fat deposition rate tended to be lower in gilts than in boars (214 vs. 247 g/d, P = 0.063), decreased by pST treatment (263 vs. 198 g/d, P < 0.001), and increased by Improvac treatment (197 vs. 264 g/d, P < 0.001). For pigs treated with both pST and Improvac, daily gain and lean tissue deposition rate was greater than for pigs that received either treatment alone, whereas fat deposition rate and feed intake did not differ from untreated control pigs. In conclusion, Improvac increased growth rate through increased lean and fat deposition, but concomitant use of Improvac and pST increased lean gain above either alone, while negating the increase in fat deposition in pigs treated with Improvac.}, number={8}, journal={Journal of Animal Science}, author={Oliver, W. T. and McCauley, L. and Harrell, R. J. and Sustert, D. and Kerton, D. J. and Dunshea, F. R.}, year={2003}, pages={1959–1966} }
 @article{mccauley_watt_suster_kerton_oliver_harrell_dunshea_2003, title={A GnRF vaccine (Improvac (R)) and porcine somatotropin (Reporcin (R)) have synergistic effects upon growth performance in both boars and gilts}, volume={54}, ISSN={["1836-5795"]}, DOI={10.1071/AR02037}, abstractNote={The use of a vaccine (Improvac) directed against gonadotrophin-releasing factor (GnRF), to prevent boar taint in male pigs, is also associated with an increase in feed consumption, growth, and higher deposition of subcutaneous fat. Daily administration of porcine somatotropin (pST) consistently increases average daily gain, and feed conversion efficiency, and reduces subcutaneous fat, particularly in gilts. The objective of this study was to examine the effect of combining these treatments in boars and gilts. Forty-eight pigs (Large White × Landrace), housed individually, were used in a 2 × 2 × 2 factorial experiment with the respective factors being vaccination with GnRF vaccine (at 14 and 18 weeks of age), daily injection with pST (from 18 weeks to slaughter at 22 weeks), and sex (boars and gilts). Performance was measured over the period of pST administration. Over the entire 28 days of the study, daily gain was lower for gilts than for boars (1216 v. 1410 g/day, P < 0.001), was increased by pST (1219 v. 1407 g/day, P < 0.001), but not significantly altered by vaccination against GnRF (1284 v. 1342 g/day, P = 0.15). However, GnRF vaccination increased daily gain in the second 2 weeks of the study, and overall, there were interactions such that the vaccine increased gain in all groups except boars not receiving pST. Feed intake over the study period was less for gilts than for boars (3139 v. 3407 g/day, P < 0.001), was decreased by pST (3537 v. 3009 g/day, P < 0.001), and increased by vaccination against GnRF (3139 v. 3407 g/day, P < 0.001). When averaged over the entire study, feed conversion ratio (FCR) was higher for gilts than for boars (2.76 v. 2.51, P = 0.013), was decreased by pST (3.05 v. 2.22, P < 0.001), but unchanged by vaccination against GnRF (2.62 v. 2.65, P = 0.70). Fat deposition was lower in gilts than in boars (335 v. 378 g/day, P = 0.037), was decreased by pST treatment ((442 v. 271 g/day, P < 0.001), and increased by vaccination against GnRF (333 v. 379 g/day, P = 0.026). In boars, fat androstenone was reduced by vaccination against GnRF (0.10 v. 2.47 μg/g, P < 0.001) and tended to be reduced by pST (1.71 v. 2.44, P = 0.10), but only vaccination against GnRF reduced skatole (0.174 v. 0.050 μg/g, P < 0.001). The combination of the treatments in all cases was at least additive, and in some cases synergistic. The concomitant vaccination against GnRF and treatment with pST affords an opportunity to increase growth rate and feed intake above control levels and above those of pigs treated with either treatment alone.}, number={1}, journal={AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH}, author={McCauley, I and Watt, M and Suster, D and Kerton, DJ and Oliver, WT and Harrell, RJ and Dunshea, FR}, year={2003}, pages={11–20} }
 @article{mathews_oliver_phillips_odle_diersen-schade_harrell_2002, title={Comparison of triglycerides and phospholipids as supplemental sources of dietary long-chain polyunsaturated fatty acids in piglets}, volume={132}, ISSN={["1541-6100"]}, DOI={10.1093/jn/131.10.3081}, abstractNote={Addition of arachidonic acid (AA) and docosahexaenoic acid (DHA) to infant formula promotes visual and neural development. This study was designed to determine whether the source of dietary long-chain polyunsaturated fatty acids (LCPUFA) affected overall animal health and safety. Piglets consumed ad libitum from 1 to 16 d of age a skim milk-based formula with different fat sources added to provide 50% of the metabolizable energy. Treatment groups were as follows: control (CNTL; no added LCPUFA), egg phospholipid (PL), algal/fungal triglyceride (TG) oils, TG plus PL (soy lecithin source) added to match phospholipid treatment (TG + PL) and essential fatty acid deficient (EFAD). Formulas with LCPUFA provided 0.6 and 0.3 g/100 g total fatty acids as AA and DHA, respectively. CNTL piglets had 40% longer ileal villi than PL piglets (P < 0.03), but the TG group was not different from the CNTL group. Gross liver histology did not differ among any of the formula-fed groups (P > 0.1). Apparent dry matter digestibility was 10% greater in CNTL, TG and TG + PL groups compared with PL piglets (P < 0.002). No differences in alanine aminotransferase were detected among treatments, but aspartate aminotransferase was elevated (P < 0.03) in PL piglets compared with TG + PL piglets. Total plasma AA concentration was greater in the TG group compared with CNTL piglets (P < 0.05). Total plasma DHA concentrations were greater in TG piglets compared with PL (P < 0.06) or CNTL (P < 0.02) piglets. These data demonstrate that the algal/fungal TG sources of DHA and AA may be a more appropriate supplement for infant formulas than the egg PL source based on piglet plasma fatty acid profiles and apparent dry matter digestibilities.}, number={10}, journal={JOURNAL OF NUTRITION}, author={Mathews, SA and Oliver, WT and Phillips, OT and Odle, J and Diersen-Schade, DA and Harrell, RJ}, year={2002}, month={Oct}, pages={3081–3089} }
 @article{oliver_mathews_phillips_jones_odle_harrell_2002, title={Efficacy of partially hydrolyzed corn syrup solids as a replacement for lactose in manufactured liquid diets for neonatal pigs}, volume={80}, DOI={10.2527/2002.801143x}, abstractNote={Feeding manufactured liquid diets to early-weaned pigs improves growth performance and reduces days to market weight compared with pigs receiving pelleted dry feed. Few alternative dietary ingredients are utilized in manufactured liquid diets other than byproducts of the dairy industry, especially for sources of carbohydrates. This experiment was designed to evaluate the efficacy of starch from partially hydrolyzed corn syrup solids (CSS), at two different levels of hydrolyzation, as a replacement for lactose in manufactured liquid diets. Forty-eight pigs were removed from sows at 1 d of age and randomly assigned to one of three treatments: 1) control with lactose as the carbohydrate source, 2) lactose replaced (gram for gram) with CSS (dextrose equivalent [DE]-20), and 3) lactose replaced with DE-42. In addition, 10 pigs were randomly removed from several litters to provide estimates of initial body composition and small intestinal variables. Twenty-four pigs were removed from the study on d 10 of treatment, and the remaining 24 pigs were removed on d 20 of treatment. Pigs averaged 9,845 +/- 191 g at d 20 of treatment regardless of dietary treatment (P > 0.20). No differences in ADG, ADFI, or feed efficiency were detected between treatment groups from d 0 to 20 (P > 0.19). Whole-body water, protein, lipid, and ash accretion rates were unaffected by dietary treatment from d 0 to 10 or from d 0 to 20 (P > 0.20). The replacement of lactose with CSS did not affect intestinal villi height or width, or crypt depth (P > 0.10). Pigs fed lactose tended to have greater lactase activity on d 10 than pigs fed CSS (P < 0.07). Also, pigs fed lactose tended to have lower oligosaccharidase activity than pigs fed the DE-20 diet on d 20 (P < 0.07). No other differences in lactase, maltase, or long oligosaccharidase specific activity on d 10 or 20 of treatment were detected (P > 0.12). Plasma urea nitrogen concentrations were unaffected by diet on d 10 and 20 of treatment. In addition, dry matter digestibility of the diets averaged approximately 85.6 +/- 0.8% and was unaffected by dietary treatment or day of treatment. These results suggest that partially hydrolyzed CSS can be used as a replacement for lactose in manufactured liquid diets for neonatal pigs.}, number={1}, journal={Journal of Animal Science}, author={Oliver, W. T. and Mathews, S. A. and Phillips, O. and Jones, E. E. and Odle, J. and Harrell, R. J.}, year={2002}, pages={143–153} }
 @article{heo_odle_oliver_kim_han_jones_1999, title={Effects of milk replacer and ambient temperature on growth performance of 14-day-old early-weaned pigs}, volume={12}, ISSN={["1976-5517"]}, DOI={10.5713/ajas.1999.908}, number={6}, journal={ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES}, author={Heo, KN and Odle, J and Oliver, W and Kim, JH and Han, IK and Jones, E}, year={1999}, month={Sep}, pages={908–913} }