Scots L. Mankin
Works (5)
Updated: July 5th, 2023 15:59
2003 journal article
Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR
TRANSGENIC RESEARCH, 12(1), 3–12.
author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
TL;DR:
Most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs, which is important for understanding the role of matrix attachment region in transgene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 journal article
New green fluorescent protein genes for plant transformation: Intron-containing, ER-localized, and soluble-modified
Plant Molecular Biology Reporter, 19(1), 13–26.
TL;DR:
Transient expression of theerGFPINTanderGFP genes is comparable in tobacco and maize suspension culture protoplasts, indicating that the PIV2 intron is spliced effectively in both monocotyledonous and dicotylingonous plant species.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2000 patent
Increasing expression of transgenes in plant cells using insulator elements
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2000 patent
Method for reducing expression variability of transgenes in plant cells
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Introduction of a plant intron into the luciferase gene of Photinus pyralis
Plant Molecular Biology Reporter, 15(2), 186–196.
TL;DR:
A new luciferase reporter gene,lucINT, is described, allowing earlier detection of gene expression in the presence of Agrobacterium during transformations in tobacco leaf discs.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
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