@article{ehling_rossbach_dunston_stark_baumer_2016, title={Allergic inflammation is augmented via histamine H4 receptor activation: The role of natural killer cells in vitro and in vivo}, volume={83}, ISSN={["1873-569X"]}, DOI={10.1016/j.jdermsci.2016.04.011}, abstractNote={Background Natural Killer cells (NK cells) are identified as pivotal mediators in allergic skin diseases and accumulate in lesions of atopic dermatitis (AD) patients. Histamine levels are increased in these lesions and histamine is involved in chemotaxis in dendritic cells and NK cells. Objective The aim of this study was to determine if the histamine H4 receptor (H4R) mediates NK cell chemotaxis and whether it influences interplay between NK cells and dendritic cells during the early phase of allergic inflammation. Methods Chemotactic function of the H4R as well as the influence of the H4R on the cytokine profile of an NK cell-dendritic cell co-culture was studied in vitro. The effect of H4R activation on NK cell migration, NK cell-dendritic cell interaction and cytokine levels in the skin was further characterized in the murine TDI model of allergic dermatitis. Additionally, the impact of the H4R on dermal NK cells was determined in the ovalbumin (OVA)- induced allergic dermatitis model, comparing wild type and H4R knockout mice. Results The selective H4R agonist ST-1006 induced NK cell chemotaxis in vitro, which was inhibited with the H4R antagonist JNJ7777120. In vivo, mice treated with TDI plus ST-1006 topically onto the ear, showed significantly enhanced ear swelling and an increased number of NK cells compared to just allergen challenged ears. CCL17 levels in the ear were also significantly increased 8 h after allergen challenge. Histology revealed that the main source for increased CCL17 were dendritic cells. These effects could be blocked using the H4R antagonist JNJ7777120. In the chronic model of allergic dermatitis, OVA induced NK cell migration into lesional skin sites. The number of NK cells was lower in OVA-sensitized H4R knockout mice compared to wild type mice. Conclusions These results identify the H4R as a new target controlling NK cell migration and NK cell-dendritic cell interaction in the skin during early allergic inflammation. These results further suggest that blocking the H4R in the skin might be beneficial in diseases like AD.}, number={2}, journal={JOURNAL OF DERMATOLOGICAL SCIENCE}, author={Ehling, Sarah and Rossbach, Kristine and Dunston, Stanley M. and Stark, Holger and Baumer, Wolfgang}, year={2016}, month={Aug}, pages={106–115} }
 @article{bizikova_linder_wofford_mamo_dunston_olivry_2015, title={Canine epidermolysis bullosa acquisita: A retrospective study of 20 cases}, volume={26}, number={6}, journal={Veterinary Dermatology}, author={Bizikova, P. and Linder, K. E. and Wofford, J. A. and Mamo, L. B. and Dunston, S. M. and Olivry, T.}, year={2015}, pages={441-} }
 @article{olivry_dunston_2015, title={Expression patterns of superficial epidermal adhesion molecules in an experimental dog model of acute atopic dermatitis skin lesions}, volume={26}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84921438113&partnerID=MN8TOARS}, DOI={10.1111/vde.12188}, abstractNote={BackgroundThe stratum corneum is critical for providing a functional skin barrier, especially in humans and dogs with atopic dermatitis. An effective barrier also depends upon intact corneodesmosomes and superficial epidermal tight junctions.}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Olivry, Thierry and Dunston, Stanley M.}, year={2015}, month={Feb}, pages={53-+} }
 @article{bizikova_olivry_mamo_dunston_2014, title={Serum autoantibody profiles of IgA, IgE and IgM in canine pemphigus foliaceus}, volume={25}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84911375280&partnerID=MN8TOARS}, DOI={10.1111/vde.12143}, abstractNote={BackgroundPemphigus foliaceus (PF) is the most common IgG‐mediated autoimmune skin disease in dogs. Studies of human PF have revealed the presence of other antigen‐specific autoantibody isotypes, thereby uncovering new avenues of investigation of the disease pathomechanism.}, number={5}, journal={VETERINARY DERMATOLOGY}, author={Bizikova, Petra and Olivry, Thierry and Mamo, Lisa B. and Dunston, Stanley M.}, year={2014}, month={Oct}, pages={471–E75} }
 @article{olivry_bizikova_paps_dunston_lerner_yosipovitch_2013, title={Cowhage can induce itch in the atopic dog}, volume={22}, ISSN={["1600-0625"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84878344758&partnerID=MN8TOARS}, DOI={10.1111/exd.12158}, abstractNote={Abstract}, number={6}, journal={EXPERIMENTAL DERMATOLOGY}, author={Olivry, Thierry and Bizikova, Petra and Paps, Judy S. and Dunston, Stan and Lerner, Ethan A. and Yosipovitch, Gil}, year={2013}, month={Jun}, pages={435–437} }
 @article{olivry_linder_wang_bizikova_bernstein_dunston_paps_casal_2012, title={Deficient plakophilin-1 expression due to a mutation in PKP1 causes ectodermal dysplasia-skin fragility syndrome in Chesapeake Bay Retriever dogs}, volume={7}, number={2}, journal={PLoS One}, author={Olivry, T. and Linder, K. E. and Wang, P. and Bizikova, P. and Bernstein, J. A. and Dunston, S. M. and Paps, J. S. and Casal, M. L.}, year={2012} }
 @article{olivry_linder_paps_bizikova_dunston_donne_mondoulet_2012, title={Validation of a novel epicutaneous delivery system for patch testing of house dust mite-hypersensitive dogs}, volume={23}, number={6}, journal={Veterinary Dermatology}, author={Olivry, T. and Linder, K. E. and Paps, J. S. and Bizikova, P. and Dunston, S. and Donne, N. and Mondoulet, L.}, year={2012} }
 @article{oberkirchner_linder_dunston_bizikova_olivry_2011, title={Metaflumizone-amitraz (Promeris)-associated pustular acantholytic dermatitis in 22 dogs: evidence suggests contact drug-triggered pemphigus foliaceus}, volume={22}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-80052525616&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2011.00974.x}, abstractNote={Abstract}, number={5}, journal={VETERINARY DERMATOLOGY}, author={Oberkirchner, Ursula and Linder, Keith E. and Dunston, Stan and Bizikova, Petra and Olivry, Thierry}, year={2011}, month={Oct}, pages={436–448} }
 @article{olivry_wofford_paps_dunston_2011, title={Stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs}, volume={22}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-79952348135&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2010.00938.x}, abstractNote={Abstract}, number={2}, journal={VETERINARY DERMATOLOGY}, author={Olivry, Thierry and Wofford, Jessica and Paps, Judy S. and Dunston, Stanley M.}, year={2011}, month={Apr}, pages={188–196} }
 @article{olivry_bizikova_dunston_bond_halliwell_loeffler_pucheu-haston_chen_marinkovich_2010, title={Clinical and immunological heterogeneity of canine subepidermal blistering dermatoses with anti-laminin-332 (laminin-5) auto-antibodies}, volume={21}, number={4}, journal={Veterinary Dermatology}, author={Olivry, T. and Bizikova, P. and Dunston, S. M. and Bond, R. and Halliwell, R. and Loeffler, A. and Pucheu-Haston, C. M. and Chen, M. and Marinkovich, M. P.}, year={2010}, pages={345–357} }
 @article{olivry_dunston_2010, title={Usefulness of Collagen IV Immunostaining for Diagnosis of Canine Epidermolysis Bullosa Acquisita}, volume={47}, ISSN={["1544-2217"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77954508167&partnerID=MN8TOARS}, DOI={10.1177/0300985809359609}, abstractNote={ In dogs, autoimmune subepidermal blistering diseases (AISBDs) encompass several distinct entities that exhibit varying clinical signs, microscopic characteristics, prognosis, and response to treatment. The identification of targeted autoantigens is usually required to make the diagnosis, but immunological tests to determine these antigens are not commercially available. Epidermolysis bullosa acquisita (EBA) is an AISBD characterized by the production of autoantibodies against collagen VII in sublamina densa anchoring fibrils. This article reports on the usefulness of collagen IV immunostaining on paraffin-embedded skin biopsies as an aid to diagnose EBA in dogs. In this disease, collagen IV, which forms the fibrous 2-dimensional network of lamina densa, is detected more commonly above subepidermal vesicles than below. In other canine AISBDs, this is rarely the case. Collagen IV immunostaining therefore offers an inexpensive means to help making a suggestive diagnosis of EBA in the absence of serological determination of the targeted autoantigen. }, number={3}, journal={VETERINARY PATHOLOGY}, author={Olivry, T. and Dunston, S. M.}, year={2010}, month={May}, pages={565–568} }
 @article{olivry_dunston_walker_alhaidari_messinger_liu_2009, title={Investigations on the nature and pathogenicity of circulating antikeratinocyte antibodies in dogs with pemphigus foliaceus}, volume={20}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-58449131572&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2008.00723.x}, abstractNote={Abstract}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Olivry, Thierry and Dunston, Stanley M. and Walker, Robina H. and Alhaidari, Zeineb and Messinger, Linda and Liu, Zhi}, year={2009}, month={Feb}, pages={42–50} }
 @article{pucheu-haston_jackson_olivry_dunston_hammerberg_2008, title={Epicutaneous sensitization with Dermatophagoides farinae induces generalized allergic dermatitis and elevated mite-specific immunoglobulin E levels in a canine model of atopic dermatitis}, volume={38}, ISSN={["0954-7894"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-40949126521&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2222.2008.02949.x}, abstractNote={Summary}, number={4}, journal={CLINICAL AND EXPERIMENTAL ALLERGY}, author={Pucheu-Haston, C. M. and Jackson, H. A. and Olivry, T. and Dunston, S. M. and Hammerberg, B.}, year={2008}, month={Apr}, pages={667–679} }
 @article{olivry_dunston_pluchino_porter_hammerberg_2008, title={Lack of detection of circulating skin-specific IgE autoantibodies in dogs with moderate or severe atopic dermatitis}, volume={122}, ISSN={["1873-2534"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-39549096018&partnerID=MN8TOARS}, DOI={10.1016/j.vetimm.2007.11.003}, abstractNote={Human patients with atopic dermatitis (AD) commonly exhibit IgE reactivity to cutaneous self-antigens. The presence of serum IgE autoantibodies appears to correlate with disease severity, and it is suspected to reflect or contribute to tissue damage. The objective of this study was to determine whether IgE autoantibodies specific for cutaneous antigens could be detected in the serum of dogs with AD. Serum was collected from 19 dogs with untreated moderate to severe AD and four specific-pathogen free (SPF) dogs. Indirect immunofluorescence was performed using normal canine skin collected at four different locations (concave ear, nose, medial thigh and lateral thorax), while Western immunoblotting was done using normal canine ear pinna epidermal and dermal extracts and reducing conditions. In both methods, IgE was detected using a monoclonal antibody specific for heat stable epitopes of canine IgE. At 1:10 dilution, specific IgE autoantibodies against cutaneous autoantigens were not detected, with either method, in AD and SPF canine sera. Either IgE autoreactivity is not associated with moderate to severe AD in dogs, or the methods employed herein were not sensitive enough to permit IgE autoantibody detection.}, number={1-2}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Olivry, Thierry and Dunston, Stanley A. and Pluchino, Kristen and Porter, Kyleigh and Hammerberg, Bruce}, year={2008}, month={Mar}, pages={182–187} }
 @article{saridomichelakis_koutinas_olivry_dunston_farmaki_koutinas_petanides_2007, title={Regional parasite density in the skin of dogs with symptomatic canine leishmaniosis}, volume={18}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-34547370380&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2007.00597.x}, abstractNote={Abstract}, number={4}, journal={VETERINARY DERMATOLOGY}, author={Saridomichelakis, Manolis N. and Koutinas, Alexander F. and Olivry, Thierry and Dunston, Stan M. and Farmaki, Rania and Koutinas, Christos K. and Petanides, Theodoros}, year={2007}, month={Aug}, pages={227–233} }
 @article{olivry_lavoy_dunston_brown_lennon_warren_prisayanh_müller_suter_dean_et al._2006, title={Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus}, volume={110}, ISSN={["0165-2427"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33644524373&partnerID=MN8TOARS}, DOI={10.1016/j.vetimm.2005.10.002}, abstractNote={The majority of human patients with pemphigus foliaceus (PF) have circulating IgG autoantibodies that target conformational epitopes on the desmosomal cadherin desmoglein-1 (dsg1). Limited studies using immunoblot techniques suggested that the principal autoantigen in dogs with PF might also be dsg1. It was the objective of this study to test this hypothesis. A comprehensive survey of canine PF sera was conducted using a novel screening strategy that detects conformational epitopes. This method consists of the ectopic expression of canine dsg1 at the surface of human 293T epithelial kidney cells and their live screening, i.e. prior to fixation. Out of seven control human PF sera that bound to canine epidermis, three (57%) contained IgG autoantibodies that recognized ectopically expressed canine dsg1 with a membrane and punctate pattern. Out of 83 canine PF sera only five (6%) contained IgG that recognized canine dsg1. Consistent with findings for human PF sera obtained in this study, autoantibody binding was conformation- and glycosylation-dependent as demonstrated by calcium chelation with EDTA and tunicamycin or wheat germ agglutinin treatment, respectively. In conclusion, these studies establish canine dsg1 as a minor autoantigen for canine PF. Antigenic epitopes appear to be conformation- and glycosylation-dependent.}, number={3-4}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Olivry, T. and LaVoy, A. and Dunston, S.M. and Brown, R.S. and Lennon, E.M. and Warren, S.J. and Prisayanh, P. and Müller, E.J. and Suter, M.M. and Dean, G.A. and et al.}, year={2006}, month={Apr}, pages={245–255} }
 @article{olivry_deangelo_dunston_clarke_mccall_2006, title={Patch testing of experimentally sensitized beagle dogs: development of a model for skin lesions of atopic dermatitis}, volume={17}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33644828769&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2006.00502.x}, abstractNote={Abstract}, number={2}, journal={VETERINARY DERMATOLOGY}, author={Olivry, T and Deangelo, KB and Dunston, SM and Clarke, KB and Mccall, CA}, year={2006}, month={Apr}, pages={95–102} }
 @article{bryden_white_dunston_burrows_olivry_2005, title={Clinical, histopathological and immunological characteristics of exfoliative cutaneous lupus erythematosus in 25 German short-haired pointers}, volume={16}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-24644462883&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2005.00468.x}, abstractNote={Abstract  Clinical, histopathological and immunological features of exfoliative cutaneous lupus erythematosus, an uncommon generalized exfoliative dermatitis occurring exclusively in German short‐haired pointers, were characterized in 25 dogs. The disease affects young adult dogs and its familial incidence strongly suggests a hereditary origin. Lesions were characterized by scaling and alopecia affecting 100 (25/25) and 76% (19/25) of dogs, respectively. Follicular casts were present in 28% (7/25) of dogs. The muzzle, pinnae and dorsum were typically affected. Generalized skin lesions were described in 52% (13/25) of dogs. Systemic signs of pain and lameness affected several dogs. Anaemia and thrombocytopenia were detected in several dogs with a more severe clinical phenotype. The most common histopathological features were hyperkeratosis and a lymphocytic interface dermatitis. Direct immunostaining revealed IgG deposition in the epidermal and follicular basement membrane of 100 (19/19) and 41% (7/17) of dogs, respectively. Circulating antifollicular and antisebaceous gland IgG antibodies were demonstrated by indirect immunostaining in 57% (4/7) of dogs. This disease usually responds poorly to immunosuppressive therapy and it has a guarded prognosis. Where outcome was recorded, 85% (10/12) of dogs were euthanased due to either a failure to respond to, or complications associated with, immunomodulatory therapy. Two affected dogs are in remission and maintained on immunomodulatory dosages of prednisolone. This study demonstrates the existence of a cellular and humoral immune response directed against the epidermal basement membrane of dogs with exfoliative cutaneous lupus erythematosus. Additional studies are required to further characterize the immunological pathogenesis of this disease.}, number={4}, journal={VETERINARY DERMATOLOGY}, author={Bryden, SL and White, SD and Dunston, SM and Burrows, AK and Olivry, T}, year={2005}, month={Aug}, pages={239–252} }
 @article{jackson_olivry_berget_dunston_bonnefont_chabanne_2004, title={Immunopathology of vesicular cutaneous lupus erythematosus in the rough collie and Shetland sheepdog: a canine homologue of subacute cutaneous lupus erythematosus in humans}, volume={15}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-4444307321&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2004.00393.x}, abstractNote={Abstract  Clinical and histological features of an erosive disease in the rough collie and Shetland sheepdog are most consistent with a vesicular variant of cutaneous lupus erythematosus (VCLE). This paper reports the immunopathological findings of canine VCLE using samples from 17 affected dogs. Lesional skin sections were stained with monoclonal antibodies specific for CD3 (11 dogs) or a panel of monoclonal antibodies specific for leukocyte antigens (two dogs). Apoptotic cells were detected using the TUNEL method in 12 cases. Direct (14 dogs) and indirect immunofluorescence tests (five dogs) were also performed. Circulating antibodies to extractable nuclear antigens (ENA) were surveyed in 11 dogs by immunoblotting and ELISA. The predominant cells at the dermal–epidermal interface were identified as CD3+ T lymphocytes expressing CD4 or CD8 and CD1+ dendritic antigen presenting cells. In 7/12 dogs (58%), apoptosis of basal keratinocyte nuclei was present. Up‐regulation of MHCII and ICAM‐1 was observed on basal keratinocytes from the two dogs examined. Direct immunofluorescence revealed deposition of immunoglobulins bound to the cytoplasm of keratinocytes (6/14 dogs; 43%), to the dermal–epidermal junction (7/14 dogs; 50%), or to superficial dermal venules (13/14 dogs; 93%). Circulating IgG auto‐antibodies targeting one or more ENA were detected in nine (82%) and eight (73%) of 11 dogs by immunoblotting and ELISA, respectively. These auto‐antibodies recognized Ro/SSA and/or La/SSB in four (36%) and six (55%) of 11 dogs respectively by these two methods. Altogether, results of these studies provide evidence supporting the hypothesis that canine VCLE is an immunological homologue of subacute cutaneous lupus erythematosus in humans.}, number={4}, journal={VETERINARY DERMATOLOGY}, author={Jackson, HA and Olivry, T and Berget, F and Dunston, SM and Bonnefont, C and Chabanne, L}, year={2004}, month={Aug}, pages={230–239} }
 @article{tobin_gardner_luther_dunston_lindsey_olivry_2003, title={A natural canine homologue of alopecia areata in humans}, volume={149}, ISSN={["1365-2133"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0141713548&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2133.2003.05610.x}, abstractNote={Background  Alopecia areata (AA) is suspected to be an autoimmune disease directed preferentially against hair follicles (HF) affecting both humans and various mammalian species. Recently, two rodent models of AA were described, namely the ageing C3H/HeJ mouse and the DEBR rat. Despite several case reports of canine AA in the literature, there has been no systematic assessment of the disease in these companion animals, and it is also not known whether dogs with AA could be useful as an outbred homologue of this disease in humans.}, number={5}, journal={BRITISH JOURNAL OF DERMATOLOGY}, author={Tobin, DJ and Gardner, SH and Luther, PB and Dunston, SM and Lindsey, NJ and Olivry, T}, year={2003}, month={Nov}, pages={938–950} }
 @article{olivry_dunston_rivierre_jackson_murphy_peters_dean_2003, title={A randomized controlled trial of misoprostol monotherapy for canine atopic dermatitis: effects on dermal cellularity and cutaneous tumour necrosis factor-alpha}, volume={14}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037310297&partnerID=MN8TOARS}, DOI={10.1046/j.1365-3164.2003.00323.x}, abstractNote={Abstract In this blinded randomized placebo‐controlled trial, 20 dogs with atopic dermatitis (AD) were given placebo (8 dogs) or misoprostol (12 dogs) at 5 µg kg−1, orally, three times daily for 3 weeks. Administration of the active drug, but not of placebo, led to a significant decrease in lesional and pruritus scores. The median reduction from baseline of both scores was ≈30%. Misoprostol therapy did not lead to decreases of dermal cell counts or skin tumour necrosis factor (TNF)α mRNA copy numbers that were significantly different from those of placebo. Skin TNFα protein production, assessed using indirect immunofluorescence, decreased or remained unchanged in dogs receiving misoprostol. In contrast, post treatment TNFα fluorescence scores were higher in all but two dogs given placebo. The changes from baseline of TNFα fluorescence scores did not correlate significantly with those of lesional or pruritus indices. These observations confirm the modest efficacy of misoprostol for treatment of canine AD and suggest that its mild anti‐allergic effects are not associated with either inhibition of inflammatory cell emigration or TNFα production.}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Olivry, T and Dunston, SM and Rivierre, C and Jackson, HA and Murphy, KM and Peters, E and Dean, GA}, year={2003}, month={Feb}, pages={37–46} }
 @article{olivry_joubeh_dunston_nishiyama_ghohestani_2003, title={Desmoglein-3 is a target autoantigen in spontaneous canine pemphigus vulgaris}, volume={12}, ISSN={["0906-6705"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0041672265&partnerID=MN8TOARS}, DOI={10.1046/j.0906-6705.2002.072.x}, abstractNote={Abstract: Pemphigus vulgaris (PV) is an autoimmune blistering skin disease of humans and companion animals. In human patients, PV is associated with the production of IgG autoantibodies specific for keratinocyte desmosomal glycoproteins of the cadherin family. The purpose of this study was to determine whether antikeratinocyte IgG autoantibodies were present in the skin and serum of dogs with PV, and also to identify the canine PV autoantigen(s) targeted by circulating autoantibodies. Eleven dogs were selected because of the microscopic demonstration of suprabasal epithelial acantholysis. Direct immunofluorescence revealed the presence of IgG autoantibodies bound to the membrane of keratinocytes in skin biopsy specimens of 8/9 dogs (89%). Using indirect immunofluorescence, serum‐circulating IgG autoantibodies were found in 10/11 (91%) and 5/11 (45%) dogs, using normal canine gingiva and cultured canine oral keratinocytes, respectively. By immunoblotting using cultured canine oral keratinocyte protein lysates, IgG autoantibodies from 7/9 (78%) tested dogs recognized a 130‐kDa antigen that comigrated with that identified by rabbit polyclonal antibodies raised against desmoglein‐3. This 130 kDa antigen was confirmed to represent the canine equivalent of human desmoglein‐3 by immunoprecipitation‐immunoblotting. The results of these studies provide evidence that the canine desmoglein‐3 homologue is a major autoantigen in dogs with PV. These observations further establish spontaneous canine PV as a natural model for research on pathogenesis, etiology and novel therapeutic approaches for this disease of humans.}, number={2}, journal={EXPERIMENTAL DERMATOLOGY}, author={Olivry, T and Joubeh, S and Dunston, SM and Nishiyama, T and Ghohestani, RF}, year={2003}, month={Apr}, pages={198–203} }
 @article{michau_proulx_rushton_olivry_dunston_gilger_davidson_2003, title={Intraocular extramedullary plasmacytoma in a cat}, volume={6}, ISSN={["1463-5224"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037629973&partnerID=MN8TOARS}, DOI={10.1046/j.1463-5224.2003.00277.x}, abstractNote={Abstract}, number={2}, journal={VETERINARY OPHTHALMOLOGY}, author={Michau, TM and Proulx, DR and Rushton, SD and Olivry, T and Dunston, SM and Gilger, BC and Davidson, MG}, year={2003}, month={Jun}, pages={177–181} }
 @article{favrot_dunston_paradis_olivry_2003, title={Isotype determination of circulating autoantibodies in canine autoimmune subepidermal blistering dermatoses}, volume={14}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037311701&partnerID=MN8TOARS}, DOI={10.1046/j.1365-3164.2003.00320.x}, abstractNote={Abstract The three most common canine autoimmune blistering skin diseases (AISBD), bullous pemphigoid (BP), mucous membrane pemphigoid (MMP) and epidermolysis bullosa acquisita (EBA) have recently been separated based on clinical, histological and immunological grounds. The objectives of this study were to determine the isotype profiles of circulating autoantibodies in these dermatoses. Serum was collected from 5 dogs with BP, 15 with MMP and 11 with EBA. All sera were tested using an indirect immunofluorescence method using salt‐split canine gingiva as substrate. Anti‐basement membrane IgG autoantibodies were detected in all patients. Among the IgG autoantibodies, IgG1 and IgG4 were encountered most frequently, while IgG2 and IgG3 were uncovered in some dogs. IgE autoantibodies were detected more often than IgA or IgM autoantibodies in any of the three entities. The predominance of IgG1, IgG4 and IgE autoantibody isotypes in dogs with AISBD is very similar to the situation found in humans with the homologous diseases.}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Favrot, C and Dunston, SM and Paradis, M and Olivry, T}, year={2003}, month={Feb}, pages={23–30} }
 @article{favrot_dunston_deslandes_paradis_olivry_2002, title={Effect of substrate selection on indirect immunofluorescence testing of canine autoimmune subepidermal blistering diseases}, volume={66}, number={1}, journal={Canadian Journal of Veterinary Research}, author={Favrot, C. and Dunston, S. and Deslandes, J. and Paradis, M. and Olivry, T.}, year={2002}, pages={26–30} }
 @article{olivry_dunston_zhang_ghohestani_2002, title={Laminin-5 is targeted by autoantibodies in feline mucous membrane (cicatricial) pemphigoid}, volume={88}, ISSN={["0165-2427"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037174074&partnerID=MN8TOARS}, DOI={10.1016/S0165-2427(02)00166-6}, abstractNote={In human and canine patients with mucous membrane (cicatricial) pemphigoid (MMP), circulating autoantibodies have been shown to target multiple epidermal basement membrane antigenic epitopes. These autoantigens include collagen XVII in humans and dogs, as well as laminin-5, laminin-6 or integrin alpha-6/beta-4 in human beings. The purpose of this study was to determine if autoantibodies targeted laminin-5 in a cat exhibiting clinical and microscopic lesions resembling those of MMP in humans. In this patient, an indirect immunofluorescence (IF) assay revealed circulating IgG and IgA autoantibodies that bound to the basement membrane zone on the dermal side of salt-split gingiva (titer 1:1000 for IgG and 1:50 for IgA). Immunoblotting, performed with affinity-purified human laminin-5, demonstrated that the autoantibodies bound the alpha-3 chain of this heterotrimer. These observations identify laminin-5 as one of the antigens recognized by circulating autoantibodies in this feline homologue of MMP in humans and dogs.}, number={3-4}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Olivry, T and Dunston, SM and Zhang, GY and Ghohestani, RF}, year={2002}, month={Sep}, pages={123–129} }
 @article{olivry_dunston_schachter_xu_nguyen_marinkovich_chan_2001, title={A spontaneous canine model of mucous membrane (cicatricial) pemphigoid, an autoimmune blistering disease affecting mucosae and mucocutaneous junctions}, volume={16}, ISSN={["0896-8411"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0034955036&partnerID=MN8TOARS}, DOI={10.1006/jaut.2001.0510}, abstractNote={Mucous membrane pemphigoid (MMP) is a rare autoimmune blistering dermatosis of humans that was previously known as cicatricial pemphigoid. It is characterized by vesicles, ulcers and scarring that affect predominantly mucosae and mucocutaneous junctions. Circulating autoantibodies recognize epitopes on basement membrane proteins such as collagen XVII or laminin-5/6. Herein, we describe the clinico-pathological and immunological characteristics of 17 dogs afflicted with a dermatosis homologous to MMP of humans. Patients exhibited vesicles and erosions predominantly on mucous membranes or mucocutaneous junctions of the mouth, nose, eyes, genitalia or anus. Histopathology revealed subepithelial vesicles with variable dermal inflammation. Direct immunofluorescence demonstrated IgG or complement at the dermoepithelial junction. Indirect immunofluorescence using salt-split epithelia permitted the detection of circulating basement membrane-specific IgG autoantibodies in 15 cases. In 11 patients, autoantibodies recognized the NC16A segment of collagen XVII, as determined by salt-split indirect immunofluorescence, immunoblotting using canine keratinocytes and ELISA with synthetic canine peptides. In one dog, autoantiodies bound to the dermal side of salt-split epithelia and recognized epitopes within the 30 kDa carboxy-terminal segment of human collagen XVII. Canine MMP, like its human counterpart, exhibits distinctive clinical signs and histopathological lesions, yet circulating autoantibodies target different antigenic epitopes. This spontaneous canine model of MMP could prove useful for studies on the pathogenesis or therapy of this human disease.}, number={4}, journal={JOURNAL OF AUTOIMMUNITY}, author={Olivry, T and Dunston, SM and Schachter, M and Xu, LT and Nguyen, N and Marinkovich, MP and Chan, LS}, year={2001}, month={Jun}, pages={411–421} }
 @article{olivry_dunston_murphy_moore_2001, title={Characterization of the inflammatory infiltrate during IgE-mediated late phase reactions in the skin of normal and atopic dogs}, volume={12}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0035260986&partnerID=MN8TOARS}, DOI={10.1046/j.1365-3164.2001.00230.x}, abstractNote={In canine and human atopic patients, the intracutaneous injection of offending allergens is followed by the development of both immediate and late‐phase reactions. The present study was performed to expand on the characterization and dynamics of inflammatory cell subsets during IgE‐mediated late‐phase reactions in canine skin. Three normal dogs and three Dermatophagoides farinae‐allergic dogs were selected for this experiment. All dogs were challenged intradermally with mite allergen, purified anticanine IgE antibodies (positive control) or phosphate‐buffered saline (negative control). Skin biopsies were obtained before and 6, 12 and 24 h post‐injection. Sections were stained with metachromatic and eosinophil‐specific histological stains. Additionally, we used an immunohistochemical method with antibodies specific for canine leukocyte antigens. This study confirmed the occurrence of a late‐phase reaction in atopic skin following allergen challenge, and in normal and atopic canine skin after intradermal injection of IgE‐specific antibodies. Whereas early emigrating dermal cells were composed chiefly of neutrophil and activated eosinophil granulocytes, there was an influx of αβ T‐lymphocytes and dermal dendritic cells in later stages of the late‐phase reactions. Because IgE‐mediated late‐phase reactions resemble spontaneous atopic canine skin lesions, both at macroscopic and microscopic levels, we propose the use of similar challenges to study the anti‐inflammatory effects of anti‐allergic drugs in a pre‐clinical setting.}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Olivry, T and Dunston, SM and Murphy, KM and Moore, PF}, year={2001}, month={Feb}, pages={49–58} }
 @article{inman_olivry_dunston_monteiro-riviere_gatto_2001, title={Electron microscopic observations of stratum corneum intercellular lipids in normal and atopic dogs}, volume={38}, ISSN={["1544-2217"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000172330100017&KeyUID=WOS:000172330100017}, DOI={10.1354/vp.38-6-720}, abstractNote={The barrier function of mammalian skin is maintained by intercellular stratum corneum lipids. In human patients with atopic dermatitis, an abnormal lipid barrier results in dry skin and increased transepidermal water loss. At this time, it is not known if a defective lipid barrier is present in atopic dogs. Normal and atopic canine skin were postfixed in ruthenium tetroxide and studied using transmission electron microscopy to determine structural differences within stratum corneum lipids. Intercellular lipid lamellae were graded on a semiquantitative scale. The deposition of stratum corneum lipid lamellae in atopic canine skin appeared markedly heterogeneous compared with that seen in normal canine skin. When present, the lamellae often exhibited an abnormal structure. The continuity and thickness of the intercellular lipid lamellae were significantly less in nonlesional atopic than in normal canine skin. These preliminary observations suggest that the epidermal lipid barrier is defective in atopic canine skin. Additional studies are needed to further characterize the biochemical defect and to possibly correct it with nutritional and/or pharmacologic intervention.}, number={6}, journal={VETERINARY PATHOLOGY}, author={Inman, AO and Olivry, T and Dunston, SM and Monteiro-Riviere, NA and Gatto, H}, year={2001}, month={Nov}, pages={720–723} }
 @article{olivry_mirsky_singleton_dunston_borrillo_xu_traczyk_rosolia_chan_2000, title={A spontaneously arising porcine model of bullous pemphigoid}, volume={292}, ISSN={["0340-3696"]}, DOI={10.1007/pl00007459}, abstractNote={Bullous pemphigoid (BP) is an IgG-mediated autoimmune blistering disease targeting the hemidesmosomal proteins bullous pemphigoid antigens 1 and 2. Currently, there is no active animal model in which to dissect the immunopathogenic mechanism. We noticed that cutaneous blistering arose spontaneously in 12 adult Yucatan minipigs. Skin lesions consisted of turgid, isolated or clustered vesicles that occasionally evolved from erythematous and pruritic patches. Histopathological examination revealed subepidermal vesicles rich in intact and degranulated eosinophils. Antigen mapping and transmission electron microscopy confirmed that dermoepidermal separation took place in the lamina lucida of the epidermal basement membrane zone. Direct immunofluorescence revealed the presence of IgG deposited linearly at the dermoepidermal junction in seven of nine skin specimens examined. Indirect immunofluorescence testing confirmed the presence, in the serum from eight of eight affected pigs, of circulating basement membrane-specific IgG autoantibodies (titers 1 : 50 to 1 : 250). Using uncleaved and salt-split lip substrates, the autoantibodies were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoelectron microscopy confirmed that circulating IgG autoantibodies recognized hemidesmosomal antigen(s). ELISA, immunoblotting and immunoadsorption demonstrated that five of eight serum samples exhibited high immunoreactivity against BPAG2-NC16A peptides. This novel porcine acquired blistering dermatosis could be proposed as a valuable model to conduct immunomechanistic studies on the natural progression of BP, correlation of autoreactive T cells or autoantibodies with disease activity, and the role of eosinophils in the blistering process, as these diseases cannot be modeled easily in human patients or in murine passive transfer models.}, number={1}, journal={ARCHIVES OF DERMATOLOGICAL RESEARCH}, author={Olivry, T and Mirsky, ML and Singleton, W and Dunston, SM and Borrillo, AKG and Xu, LT and Traczyk, T and Rosolia, DL and Chan, LS}, year={2000}, month={Jan}, pages={37–45} }
 @article{olivry_dunston_fahey_nguyen_marinkovich_2000, title={Autoantibodies against the processed ectodomain of collagen XVII (BPAG2, BP180) define a canine homologue of linear IgA disease of humans}, volume={37}, ISSN={["0300-9858"]}, DOI={10.1354/vp.37-4-302}, abstractNote={ Linear IgA disease (LAD) is an acquired autoimmune subepidermal blistering dermatosis that affects human children and adults. In contrast to bullous pemphigoid, in which autoantibodies recognize transmembrane type XVII collagen (BP180, BPAG2), LAD is associated with skin-fixed and circulating IgA autoantibodies that target LAD-1, the processed extracellular form of type XVII collagen. An immunologic homologue of LAD in humans was identified in two dogs according to the following criteria: 1) erosive, ulcerative, and crusted lesions seen on the face, in the oral cavity, and on the extremities, 2) dermoepidermal clefting present in the basement membrane lamina lucida without inflammation or with mild neutrophilic infiltration, 3) basement membrane-fixed IgG and/or IgA antibodies, and 4) circulating IgA and IgG autoantibodies that target the 120-kd soluble protein LAD-1. The present study establishes unequivocally the existence of a naturally occurring canine model of LAD of humans. }, number={4}, journal={VETERINARY PATHOLOGY}, author={Olivry, T and Dunston, SM and Fahey, M and Nguyen, N and Marinkovich, MP}, year={2000}, month={Jul}, pages={302–309} }
 @article{rivierre_dunston_olivry_2000, title={Effects of a 1 percent hydrocortisone conditioner on the prevention of immediate and late-phase reactions in canine skin}, volume={147}, number={26}, journal={Veterinary Record}, author={Rivierre, C. and Dunston, S. M. and Olivry, T.}, year={2000}, pages={739–742} }
 @article{olivry_borrillo_xu_dunston_slovis_affolter_demanuelle_chan_2000, title={Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)}, volume={73}, ISSN={["1873-2534"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0033982506&partnerID=MN8TOARS}, DOI={10.1016/S0165-2427(99)00151-8}, abstractNote={Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans.}, number={1}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Olivry, T and Borrillo, AKG and Xu, LT and Dunston, SM and Slovis, NM and Affolter, VK and DeManuelle, TC and Chan, LS}, year={2000}, month={Jan}, pages={45–52} }
 @article{olivry_petersen_dunston_chen_2000, title={Novel localised variant of canine epidermolysis bullosa acquisita}, volume={146}, ISSN={["0042-4900"]}, DOI={10.1136/vr.146.7.193}, abstractNote={Ultrasonography for evaluating ovine joint disease THE elbow, carpal, fetlock and stifle joints of six normal sheep were examined with a 7*5 MHz transducer with a stand-off pad, and the ultrasonographic appearance was compared with postmortem findings. In four of them the joints were examined postmortem before and after the joint capsules had been distended with 10 ml isotonic saline; this procedure made the capsules visible as a 2 mm thick echogenic line. Five sheep with chronic arthritis/synovitis were examined in the same way. The gross thickening of the joint capsule was visible as a hyperechoic band up to 20 mm thick. It is conduded that ultrasonography is useful in the evaluation of chronic joint disease in sheep, and may be valuable in assessing the prognosis for sheep with chronic arthritis/synovitis. MACRAE, A. I & SCOTT, P. R. (1999) Veterinary Journal 158, 135 The VeterInary Record, February 12, 2000 194}, number={7}, journal={VETERINARY RECORD}, author={Olivry, T and Petersen, A and Dunston, SM and Chen, M}, year={2000}, month={Feb}, pages={193–194} }
 @article{olivry_savary_murphy_dunston_chen_1999, title={Bullous systemic lupus erythematosus (type I) in a dog}, volume={145}, ISSN={["0042-4900"]}, DOI={10.1136/vr.145.6.165}, abstractNote={In human patients with systemic lupus erythematosus, cutaneous subepidermal blistering can occur because of the production of antibodies specific for basement membrane antigens. This condition is referred to as bullous systemic lupus erythematosus (BSLE). A dog was diagnosed with BSLE because it because of the production of antibodies specific for basement membrane antigens. This condition is referred to as bullous systemic lupus erythematosus (BSLE). A dog was diagnosed with BSLE because it fulfilled the following criteria: (i) a diagnosis of systemic lupus erythematosus by standard methods; (ii) an acquired, vesicular, erosive and ulcerative eruption; (iii) microscopical subepidermal vesicles with neutrophil‐predominant inflammation at the dermo‐epidermal junction; (iv) deposition of IgG at the epidermal basement membrane zone; and (v) circulating IgG autoantibodies against type VIl collagen. Anti‐collagen Vil type l‐BSLE therefore needs to be considered as a possible differential diagnosis for canine autoimmune subepidermal blistering diseases.}, number={6}, journal={VETERINARY RECORD}, author={Olivry, T and Savary, KCM and Murphy, KM and Dunston, SM and Chen, M}, year={1999}, month={Aug}, pages={165–169} }
 @article{olivry_chan_xu_chace_dunston_fahey_marinkovich_1999, title={Novel feline autoimmune blistering disease resembling bullous pemphigoid in humans: IgG autoantibodies target the NC16A ectodomain of type XVII collagen (BP180/BPAG2)}, volume={36}, ISSN={["0300-9858"]}, DOI={10.1354/vp.36-4-328}, abstractNote={In humans and dogs, bullous pemphigoid (BP) is an autoimmune blistering disease associated with the production of basement membrane autoantibodies that target the 180–kd type XVII collagen (BP180, BPAG2) and/or the 230-kd plakin epidermal isoform BPAGle (BP230). In two adult cats, an acquired dermatosis and stomatitis was diagnosed as BP subsequent to the fulfillment of the following criteria: 1) presence of cutaneous vesicles, erosions, and ulcers; 2) histologic demonstration of subepidermal vesiculation with inflammatory cells, including eosinophils; 3) in vivo deposition of IgG autoantibodies at the epidermal basement membrane zone; and 4) serum IgG autoantibodies targeting a 180-kd epidermal protein identified as type XVII collagen. In both cats, the antigenic epitopes targeted by IgG autoantibodies were shown to be situated in the NC16A ectodomain of type XVII collagen, a situation similar to that of humans and dogs with BP. Feline BP therefore can be considered a clinical, histopathologic, and immunologic homologue of BP in humans and dogs.}, number={4}, journal={VETERINARY PATHOLOGY}, author={Olivry, T and Chan, LS and Xu, L and Chace, P and Dunston, SM and Fahey, M and Marinkovich, MP}, year={1999}, month={Jul}, pages={328–335} }
 @article{olivry_dunston_marinkovich_1999, title={Reduced anchoring fibril formation and collagen VII immunoreactivity in feline dystrophic epidermolysis bullosa}, volume={36}, ISSN={["0300-9858"]}, DOI={10.1354/vp.36-6-616}, abstractNote={ Dystrophic epidermolysis bullosa was diagnosed in a cat with juvenile-onset epithelial sloughing of the oral mucosa, footpads, and haired skin. Dermoepidermal separation occurred in the absence of inflammation or cytolysis of basal epidermal cells. Collagen IV-specific immunostaining corroborated the fact that clefting took place below the epidermal basement membrane. Ultrastructural examination revealed that the proband's anchoring fibrils exhibited a filamentous morphology and were decreased in number compared with those in a normal cat. Finally, the attenuated immunoreactivity for collagen VII in our patient led us to suspect that its encoding gene, COL7A1, could be mutated in this case of feline dystrophic epidermolysis bullosa. }, number={6}, journal={VETERINARY PATHOLOGY}, author={Olivry, T and Dunston, SM and Marinkovich, MP}, year={1999}, month={Nov}, pages={616–618} }