Robert A. Ascenzi

Works (4)

Updated: July 5th, 2023 16:02

2003 article

Analysis of Trans-Silencing Interactions Using Transcriptional Silencers of Varying Strength and Targets With and Without Flanking Nuclear Matrix Attachment Regions

Ascenzi, R., Ülker, B., Todd, J. J., Sowinski, D. A., Schimeneck, C. R., Allen, G. C., … Thompson, W. F. (2003, June 1). Transgenic Research, Vol. 12, pp. 305–318.

By: R. Ascenzi n, B. Ülker n, J. Todd n, D. Sowinski n, C. Schimeneck n, G. Allen n, A. Weissinger n, W. Thompson n 

author keywords: matrix attachment region; tobacco; transcriptional gene silencing; transgene expression; trans-silencing
MeSH headings : Gene Silencing; Inheritance Patterns; Matrix Attachment Regions / genetics; Seedlings / genetics; Sequence Homology; Silencer Elements, Transcriptional; Tobacco / genetics; Transcription, Genetic
topics (OpenAlex): Plant Virus Research Studies; Plant tissue culture and regeneration; Plant Molecular Biology Research
TL;DR: The results are consistent with a model in which transcriptional silencing is triggered by transcription of sequences during the early stages of embryo or seedling development. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 article

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions

Ascenzi, R., Ingram, J. L., Massel, M., Thompson, W. F., Spiker, S., & Weissinger, A. K. (2001, October 1). Transgenic Research, Vol. 10, pp. 465–470.

By: R. Ascenzi n, J. Ingram  n, M. Massel n, W. Thompson n , S. Spiker n & A. Weissinger n

author keywords: cell differentiation and proliferation; chromatin; matrix; scaffold attachment region (MAR/SAR); plant high mobility group-I/Y protein (HMG-I/Y); transgene expression; tobacco
MeSH headings : Cell Differentiation; Cells, Cultured; Gene Expression; HMGA1a Protein / genetics; HMGA1a Protein / metabolism; Plants, Genetically Modified; Tobacco / cytology; Tobacco / genetics
topics (OpenAlex): Plant tissue culture and regeneration; Transgenic Plants and Applications; Plant Genetic and Mutation Studies
TL;DR: It appears that tobacco nuclear matrix attachment region function is not enhanced simply because cells in culture divide rapidly and are not differentiated. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1999 journal article

Molecular genetic analysis of the drought-inducible linker histone variant in Arabidopsis thaliana

Plant Molecular Biology, 41(2), 159–169.

By: R. Ascenzi & J. Gantt

Source: NC State University Libraries
Added: August 6, 2018

1999 article

Subnuclear distribution of the entire complement of linker histone variants in Arabidopsis thaliana

Ascenzi, R., & Gantt, J. S. (1999, November 4). Chromosoma.

By: R. Ascenzi* & J. Gantt*

MeSH headings : Antibody Specificity; Arabidopsis / genetics; Arabidopsis Proteins; Cell Nucleus / genetics; Cell Nucleus / metabolism; Chromatin / chemistry; Chromatin / metabolism; Cross-Linking Reagents / chemistry; DNA, Plant / immunology; DNA, Plant / isolation & purification; DNA, Ribosomal / metabolism; Fluorescent Antibody Technique, Indirect; Formaldehyde / chemistry; Gene Dosage; Histones / genetics; Histones / immunology; Histones / metabolism; In Situ Hybridization; Interphase; Plant Proteins / genetics; Plant Proteins / metabolism; Precipitin Tests; Repetitive Sequences, Nucleic Acid; Telomere / metabolism; rab GTP-Binding Proteins
topics (OpenAlex): Genomics and Chromatin Dynamics; Plant Molecular Biology Research; Chromosomal and Genetic Variations
TL;DR: The results suggest that plants, as has been shown for animals, possess a variant linker histone that is differentially localized and is bound to the compacted nucleosomal arrays at the telomere but with reduced stoichiometry. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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