@article{thoma_george_duvall_wu_whitaker_oliver_mukerjee_brantley_spann_bell_et al._2019, title={Rubbertown Next Generation Emissions Measurement Demonstration Project}, volume={16}, ISSN={["1660-4601"]}, DOI={10.3390/ijerph16112041}, abstractNote={Industrial facilities and other sources can emit air pollutants from fugitive leaks, process malfunctions and area sources that can be difficult to understand and to manage. Next generation emissions measurement (NGEM) approaches executed near facilities are enabling new ways to assess these sources and their impacts to nearby populations. This paper describes complementary uses of emerging NGEM systems in a Louisville, KY industrial district (Rubbertown), focusing on an important area air toxic, 1,3-butadiene. Over a one-year deployment starting in September 2017, two-week average passive samplers (PSs) at 11 sites showed both geospatial and temporal trends. At 0.24 ppbv annual average 1,3-butadiene concentration, a group of PSs located near facility fence lines was elevated compared to a PS group located in the community and upwind from facilities (0.07 ppbv average). Two elevated PS periods capturing emission events were examined using time-resolved NGEM approaches as case studies. In one event a 1.18 ppbv PS reading was found to be relatively localized and was caused by a multiday emission from a yet to be identified, non-facility source. In the other event, the airshed was more broadly impacted with PS concentrations ranging from 0.71 ppbv for the near-facility group to 0.46 ppbv for the community group. This case was likely influenced by a known emission event at an industrial facility. For both case studies, air pollutant and wind data from prototype NGEM systems were combined with source location models to inform the emission events. This research illustrates the power of applying NGEM approaches to improve both the understanding of emissions near sources and knowledge of impacts to near-source communities.}, number={11}, journal={INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH}, author={Thoma, Eben and George, Ingrid and Duvall, Rachelle and Wu, Tai and Whitaker, Donald and Oliver, Karen and Mukerjee, Shaibal and Brantley, Halley and Spann, Jane and Bell, Tiereny and et al.}, year={2019}, month={Jun} }
 @article{tang_2003, title={Recent advances in the molecular genetics of resin biosynthesis and genetic engineering strategies to improve defenses in conifers}, volume={14}, ISBN={1007-662X}, number={2}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2003}, pages={171} }
 @article{tang_tian_2003, title={Transgenic loblolly pine (Pinus taeda L.) plants expressing a modified delta-endotoxin gene of Bacillus thuringiensis with enhanced resistance to Dendrolimus punctatus Walker and Crypyothelea formosicola Staud}, volume={54}, ISSN={["1460-2431"]}, DOI={10.1093/jxb/erg071}, abstractNote={A synthetic version of the CRY1Ac gene of Bacillus thuringiensis has been used for the transformation of loblolly pine (Pinus taeda L.) using particle bombardment. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Expression vector pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) CRY1Ac coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator sequences, and the neomycin phosphotransferase II (NPTII) gene controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected on media with kanamycin. Shoot regeneration was induced from the kanamycin-resistant calli, and transgenic plantlets were then produced. More than 60 transformed plants from independent transformation events were obtained for each loblolly pine genotype tested. The integration and expression of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern hybridization, by Northern blot analysis, and by Western blot analysis. Effective resistance of transgenic plants against Dendrolimus punctatus Walker and Crypyothelea formosicola Staud was verified in feeding bioassays with the insects. The transgenic plants recovered could represent a good opportunity to analyse the impact of genetic engineering of pine for sustainable resistance to pests using a B. thuringiensis insecticidal protein. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform.}, number={383}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, author={Tang, W and Tian, YC}, year={2003}, month={Feb}, pages={835–844} }
 @article{tang_samuels_2002, title={Genetic transformation of Pinus taeda by particle bombardment}, volume={13}, ISBN={1007-662X}, number={2}, journal={Journal of Forestry Research}, author={Tang, W. and Samuels, V.}, year={2002}, pages={91} }
 @article{tang_2002, title={Regeneration of transgenic loblolly pine expressing genes for salt tolerance}, volume={13}, ISBN={1007-662X}, number={1}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2002}, pages={1} }
 @article{tang_2001, title={Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)}, volume={11}, ISSN={["1001-0602"]}, DOI={10.1038/sj.cr.7290092}, abstractNote={This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for beta-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers.}, number={3}, journal={CELL RESEARCH}, author={Tang, W}, year={2001}, month={Sep}, pages={237–243} }
 @article{tang_2001, title={Conifer genetic engineering: Particle bombardment and Agrobacterium-mediated gene transfer and its application in future forests}, volume={12}, ISBN={1007-662X}, number={4}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2001}, pages={220} }
 @article{tang_2001, title={Enhanced somatic embryogenesis and plant regeneration from embryogenic cultures derived from mature loblolly pine zygotic embryos by suspension culture and medium selection}, volume={3}, ISBN={1008-1321}, number={2}, journal={Forestry Studies in China}, author={Tang, W.}, year={2001}, pages={1} }
 @article{tang_samuels_ogbon_mccoy_2001, title={Functional genomics: Gene identification via T-DNA mediated gene trap tagging in plants}, volume={12}, ISBN={1007-662X}, number={1}, journal={Journal of Forestry Research}, author={Tang, W. and Samuels, V. and Ogbon, J. and McCoy, A.}, year={2001}, pages={1} }
 @misc{tang_luo_sanmuels_2001, title={Gene silencing: Double-stranded RNA mediated mRNA degradation and gene inactivation}, volume={11}, ISSN={["1748-7838"]}, DOI={10.1038/sj.cr.7290084}, abstractNote={The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double-stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methylation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.}, number={3}, journal={CELL RESEARCH}, author={Tang, W and Luo, XY and Sanmuels, V}, year={2001}, month={Sep}, pages={181–186} }
 @article{tang_ogbon_mccoy_2001, title={Genetic engineering and lignin biosynthetic regulation in forest tree species}, volume={12}, ISBN={1007-662X}, number={2}, journal={Journal of Forestry Research}, author={Tang, W. and Ogbon, J. and McCoy, A.}, year={2001}, pages={75} }
 @article{tang_whetten_sederoff_2001, title={Genotypic control of high-frequency adventitious shoot regeneration via somatic organogenesis in loblolly pine}, volume={161}, ISSN={0168-9452}, url={http://dx.doi.org/10.1016/s0168-9452(01)00394-6}, DOI={10.1016/S0168-9452(01)00394-6}, abstractNote={Mature zygotic embryos of 24 genotypes of loblolly pine (Pinus taeda L.) were used as explants to establish an adventitious shoot regeneration system through somatic organogenesis. Callus formation frequencies of 18.2 (genotype 11-1103) -77.7% (genotype 7-100) have been induced from mature zygotic embryos of all genotypes tested on callus induction medium (basal salts) containing 2,4-dichlorophenoxyacetic acid (2,4-D) or alpha-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and kinetin. Adventitious shoot regeneration via organogenesis with the frequency of 5.4 (genotype 11-1103 and 7-2) -77.2% (genotype 8-1082) was obtained from callus and tissue cultures derived from mature zygotic embryos of 24 genotypes of loblolly pine. The highest mean number of 18 adventitious buds per piece of callus 0.5x0.5 cm(2) in size was obtained from genotype 8-1082. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l BA. After rooting, regenerated plantlets were established in soil. These results suggested that adventitious shoot regeneration via somatic organogenesis was influenced by the genotypes. The in vitro regeneration procedure established in this investigation could be used for clonal micropropagation of some genotypes of loblolly pine, as well as for establishing a transformation system in coniferous species.}, number={2}, journal={Plant Science}, publisher={Elsevier BV}, author={Tang, Wei and Whetten, Ross and Sederoff, Ron}, year={2001}, month={Jul}, pages={267–272} }
 @article{tang_guo_2001, title={In vitro propagation of loblolly pine via direct somatic organogenesis from mature cotyledons and hypocotyls}, volume={33}, ISSN={["0167-6903"]}, DOI={10.1023/A:1010764816523}, number={1}, journal={PLANT GROWTH REGULATION}, author={Tang, W and Guo, ZC}, year={2001}, month={Jan}, pages={25–31} }
 @article{tang_2001, title={In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets}, volume={20}, number={2}, journal={Plant Cell Reports (Online)}, author={Tang, W.}, year={2001}, pages={163–168} }
 @article{tang_guo_ouyang_2001, title={Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos}, volume={37}, ISSN={["1054-5476"]}, DOI={10.1007/s11627-001-0097-8}, number={5}, journal={IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT}, author={Tang, W and Guo, ZC and Ouyang, F}, year={2001}, pages={558–563} }
 @article{tang_sederoff_whetten_2001, title={Regeneration of transgenic loblolly pine (Pinus taeda L.) from zygotic embryos transformed with Agrobacterium tumefaciens}, volume={213}, ISSN={["1432-2048"]}, DOI={10.1007/s004250100566}, abstractNote={Embryos of 24 open-pollinated families of loblolly pine (Pinus teade L.) were used as explants to conduct in vitro regeneration. Then, Agrobacterium tumefaciens strain GV3101 harboring the plasmid pPCV6NFHygGUSINT was used to transform mature zygotic embryos of seven families of loblolly pine. The frequency of transformation varied among families infected with A. tumefaciens. The highest frequency (100%) of transient beta-glucuronidase (GUS)-expressing embryos was obtained from family 11-1029 with over 300 blue spots per embryo. Expression of the GUS reporter gene was observed in cotyledons, hypocotyls, and radicles of co-cultivated mature zygotic embryos, as well as in callus and shoots derived from co-cultivated mature zygotic embryos. Ninety transgenic plants were regenerated from hygromycin-resistant callus derived from families W03. 8-1082 and 11-1029. and 19 transgenic plantlets were established in soil. The presence of the GUS gene in the plant genome was confirmed by polymerase chain reaction. Southern blot, and plant DNA/T-DNA junction analysis. These results suggest that an efficient A. tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine has been developed and that this transformation system could be useful for future studies on transferring economically important genes to loblolly pine.}, number={6}, journal={PLANTA}, author={Tang, W and Sederoff, R and Whetten, R}, year={2001}, month={Oct}, pages={981–989} }
 @article{tang_2001, title={Somatic embryogenesis and peroxidase activity of desiccation tolerant mature somatic embryos of loblolly pine}, volume={12}, ISBN={1007-662X}, number={3}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2001}, pages={147} }
 @article{tang_2000, title={Genetic transformation of loblolly pine using mature zygotic embryo explants by Agrobacterium tumefaciens}, volume={11}, ISBN={1007-662X}, number={4}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2000}, pages={215} }
 @article{tang_2000, title={High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng}, volume={19}, number={7}, journal={Plant Cell Reports (Online)}, author={Tang, W.}, year={2000}, pages={727–732} }
 @article{tang_2000, title={Micropropagation of loblolly pine by somatic organogenesis and RAPD analysis of regenerated plantlets}, volume={11}, ISBN={1007-662X}, number={1}, journal={Journal of Forestry Research}, author={Tang, W.}, year={2000}, pages={1} }
 @article{tang_2000, title={Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos}, volume={36}, number={6}, journal={In Vitro Cellular & Developmental Biology. Plant}, author={Tang, W.}, year={2000}, pages={488–491} }
 @article{tang_fan_1999, title={Plant regeneration via organogenesis from six families of loblolly pine}, volume={58}, ISSN={["0167-6857"]}, DOI={10.1023/A:1006392330738}, number={3}, journal={PLANT CELL TISSUE AND ORGAN CULTURE}, author={Tang, W and Fan, QY}, year={1999}, pages={223–226} }