@article{hincha_livingston_premakumar_zuther_obel_cacela_heyer_2007, title={Fructans from oat and rye: Composition and effects on membrane stability during drying}, volume={1768}, ISSN={["1879-2642"]}, DOI={10.1016/j.bbamem.2007.03.011}, abstractNote={Fructans have been implicated in the abiotic stress tolerance of many plant species, including grasses and cereals. To elucidate the possibility that cereal fructans may stabilize cellular membranes during dehydration, we used liposomes as a model system and isolated fructans from oat (Avena sativa) and rye (Secale cereale). Fructans were fractionated by preparative size exclusion chromatography into five defined size classes (degree of polymerization (DP) 3 to 7) and two size classes containing high DP fructans (DP > 7 short and long). They were characterized by high performance liquid chromatography (HPLC) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The effects of the fructans on liposome stability during drying and rehydration were assessed as the ability of the sugars to prevent leakage of a soluble marker from liposomes and liposome fusion. Both species contain highly complex mixtures of fructans, with a DP up to 17. The two DP > 7 fractions from both species were unable to protect liposomes, while the fractions containing smaller fructans were protective to different degrees. Protection showed an optimum at DP 4 and the DP 3, 4, and 5 fractions from oat were more protective than all other fractions from both species. In addition, we found evidence for synergistic effects in membrane stabilization in mixtures of low DP with DP > 7 fructans. The data indicate that cereal fructans have the ability to stabilize membranes under stress conditions and that there are size and species dependent differences between the fructans. In addition, mixtures of fructans, as they occur in living cells may have protective properties that differ significantly from those of the purified fractions.}, number={6}, journal={BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES}, author={Hincha, Dirk K. and Livingston, David P., III and Premakumar, Ramaswamy and Zuther, Ellen and Obel, Nicolai and Cacela, Constanca and Heyer, Arnd G.}, year={2007}, month={Jun}, pages={1611–1619} }
 @article{herman_rotter_premakumar_elwinger_bae_ehler-king_chen_livingston_2006, title={Additional freeze hardiness in wheat acquired by exposure to-3 degrees C is associated with extensive physiological, morphological, and molecular changes}, volume={57}, ISSN={["1460-2431"]}, DOI={10.1093/jxb/erl111}, abstractNote={Cold-acclimated plants acquire an additional 3-5 degrees C increase in freezing tolerance when exposed to -3 degrees C for 12-18 h before a freezing test (LT50) is applied. The -3 degrees C treatment replicates soil freezing that can occur in the days or weeks leading to overwintering by freezing-tolerant plants. This additional freezing tolerance is called subzero acclimation (SZA) to differentiate it from cold acclimation (CA) that is acquired at above-freezing temperatures. Using wheat as a model, results have been obtained indicating that SZA is accompanied by changes in physiology, cellular structure, the transcriptome, and the proteome. Using a variety of assays, including DNA arrays, reverse transcription-polymerase chain reaction (RT-PCR), 2D gels with mass spectroscopic identification of proteins, and electron microscopy, changes were observed to occur as a consequence of SZA and the acquisition of added freezing tolerance. In contrast to CA, SZA induced the movement of intracellular water to the extracellular space. Many unknown and stress-related genes were upregulated by SZA including some with obvious roles in SZA. Many genes related to photosynthesis and plastids were downregulated. Changes resulting from SZA often appeared to be a loss of rather than an appearance of new proteins. From a cytological perspective, SZA resulted in alterations of organelle structure including the Golgi. The results indicate that the enhanced freezing tolerance of SZA is correlated with a wide diversity of changes, indicating that the additional freezing tolerance is the result of complex biological processes.}, number={14}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, author={Herman, Eliot M. and Rotter, Kelsi and Premakumar, Ramaswamy and Elwinger, G. and Bae, Rino and Ehler-King, Linda and Chen, Sixue and Livingston, David P., III}, year={2006}, month={Nov}, pages={3601–3618} }
 @article{livingston_premakumar_tallury_2006, title={Carbohydrate partitioning between upper and lower regions of the crown in oat and rye during cold acclimation and freezing}, volume={52}, ISSN={["1090-2392"]}, DOI={10.1016/j.cryobiol.2005.11.001}, abstractNote={Carbohydrates have long been recognized as an important aspect of freezing tolerance in plants but the association between these two factors is often ambiguous. To help clarify the relationship, the allocation of carbohydrates between specific tissues within the over wintering organ (crown) of winter cereals was measured. A winter-hardy and non-winter-hardy oat (Avena sativa L.), and a rye (Secale cereale L.) cultivar were grown and frozen under controlled conditions. Crown tissue was fractionated into an upper portion, called the apical region, and a lower portion, called the lower crown. These tissues were ground in liquid N and extracted with water. Extracts were analyzed by HPLC for the simple sugars, sucrose, glucose, fructose, and for fructan of various size classes. After 3 weeks of cold acclimation at 3 degrees C, carbohydrates accounted for approximately 40% of the dry weight of oats and 60% of the dry weight of rye. The apical region, which is the tissue within the crown that acclimates to the greatest extent, was generally 10% higher in total carbohydrates than the lower crown. During a mild freeze, various carbohydrates were allocated differently between specific tissues in the three genotypes. When frozen, fructan generally decreased to a greater extent in the lower crown than in the apical region but sugars increased more in the apical region than in the lower crown. Results suggest that to understand how carbohydrates relate to freezing tolerance, regions of the crown that endure freezing stress differently should be compared.}, number={2}, journal={CRYOBIOLOGY}, author={Livingston, DP and Premakumar, R and Tallury, SP}, year={2006}, month={Apr}, pages={200–208} }
 @article{livingston_premakumar_tallury_2005, title={Carbohydrate concentrations in crown fractions from winter oat during hardening at sub-zero temperatures}, volume={96}, ISSN={["1095-8290"]}, DOI={10.1093/aob/mci167}, abstractNote={BACKGROUND AND AIMS
Contradictory results in correlation studies of plant carbohydrates with freezing tolerance may be because whole crown tissue is analysed for carbohydrates while differences exist in the survival of specific tissue within the crown. The aim of this study was to see if carbohydrate changes in tissue within oat crowns during second phase hardening (sub-zero hardening) are tissue specific.


METHODS
The lower portion of oat (Avena sativa) crowns was exposed to mild grinding in a blender and the remaining crown meristem complex, consisting of tough root-like vessels, was ground in a device developed specifically for grinding cereal crown tissue. Carbohydrates were extracted by water and measured by HPLC. Carbohydrate concentrations were compared in the two regions of the crown before and after hardening at sub-zero temperatures.


KEY RESULTS
Fructan of all size classes except DP>6 decreased during sub-zero hardening in both stems (base of leaf sheath) and crown meristem complex. Total simple sugar increase, including sucrose, was significantly higher in the crown meristem complex than in the stem.


CONCLUSIONS
Results support the hypothesis that carbohydrate change in mildly frozen plants is tissue specific within crowns and underscore the need to evaluate specific tissue within the crown when correlating the biochemistry of plants with freezing tolerance.}, number={2}, journal={ANNALS OF BOTANY}, author={Livingston, D and Premakumar, R and Tallury, SP}, year={2005}, month={Aug}, pages={331–335} }
 @article{livingston_tallury_premkumar_owens_olien_2005, title={Changes in the histology of cold-hardened oat crowns during recovery from freezing}, volume={45}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2004.0579}, abstractNote={The survival of cereal crops during winter depends primarily on the ability of tissue in the crown to withstand various stresses encountered during freezing. Freeze‐induced damage to specific regions of oat (Avena sativa L.) crowns was evaluated by sectioning plants at various stages of recovery after they had been grown and frozen under controlled conditions. Our results confirmed those reported for barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) that the apical meristem was apparently the tissue in the crown most tolerant of freezing. Photographs of sections during recovery provided evidence that the apical meristem within the crown survived freezing in plants that were rated as nonsurvivors. Closer examination revealed abnormal nuclei in many cells of plants that had been frozen. These cells with condensed and dark red chromatin resembled the description of nuclear pycnosis found in mammalian cells damaged by radiation, extreme abiotic stress, and various carcinogens. The crown meristem complex was separated from the crown and fractionated into two regions: the upper portion of the crown meristem complex, called the apical region, and the lower portion called the crown core. The dry weight of both the apical region and crown core increased during cold‐hardening but the increase in dry weight was higher in the crown core than in the apical region. During cold‐hardening the percentage of total water freezing at −2°C became lower and after 3 wk was 50 and 47% in the apical region and the crown core, respectively. The initial freezing rate of the apical region was higher than that of the crown core and reached equilibrium about 2 h earlier than the crown core. Differences are discussed in relation to the freezing survival of specific tissue.}, number={4}, journal={CROP SCIENCE}, author={Livingston, DP and Tallury, SP and Premkumar, R and Owens, SA and Olien, CR}, year={2005}, pages={1545–1558} }
 @article{brooks_vaughn_griffey_price_pridgen_rohrer_brann_rucker_behl_sisson_et al._2005, title={Registration of 'Doyce' hulless barley}, volume={45}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2005.0792}, abstractNote={Crop ScienceVolume 45, Issue 2 p. 792-793 Registrations of Cultivars Registration of 'Doyce' Hulless Barley W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorH.E. Vivar, H.E. Vivar Retired Barley Breeder, ICARDA-CIMMYT, Lisboa 27, Colonia Juarez, Apdo. Postal 6-641, 06600 MexicoSearch for more papers by this authorR.L. Paris, R.L. Paris USDA-ARS, P. O. Box 345, Stoneville, MS, 38776Search for more papers by this author W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorH.E. Vivar, H.E. Vivar Retired Barley Breeder, ICARDA-CIMMYT, Lisboa 27, Colonia Juarez, Apdo. Postal 6-641, 06600 MexicoSearch for more papers by this authorR.L. Paris, R.L. Paris USDA-ARS, P. O. Box 345, Stoneville, MS, 38776Search for more papers by this author First published: 01 March 2005 https://doi.org/10.2135/cropsci2005.0792Citations: 6 Registered by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL References 1Brooks, W.S., Registration of 'Price' barley. Crop Sci. (2005) 45, 791– 792 http://doi.org/10.2135/cropsci2005.0791, 2Caldwell, R.M., Registration of 'Harrison' barley. :. Crop Sci. (1966) 6, 387http://doi.org/10.2135/cropsci1966.0011183X000600040038x, 3Finkner, V.C., Registration of 'Barsoy' barley. :. Crop Sci. (1968) 8, 397http://doi.org/10.2135/cropsci1968.0011183X000800030047x, 4Jensen, N.F., Registration of 'Wong' barley. :. Crop Sci. (1964) 4, 238http://doi.org/10.2135/cropsci1964.0011183X000400020052x 5Price, A.M., a. Registration of 'Pamunkey' barley. :. Crop Sci. (1996) 36, 1077http://doi.org/10.2135/cropsci1996.0011183X0036000400050x, 6Price, A.M., b. Registration of 'Callao' barley. :. Crop Sci. (1996) 36, 1077http://doi.org/10.2135/cropsci1996.0011183X0036000400050x, 7Starling, T.M., Registration of 'Hanover' barley. :. Crop Sci. (1970) 1, 456http://doi.org/10.2135/cropsci1961.0011183X000100060021x 8Starling, T.M., a. Registration of 'Monroe' barley. :. Crop Sci. (1980) 20, 284http://doi.org/10.2135/cropsci1980.0011183X002000020040x, 9Starling, T.M., b. Registration of 'Surry' barley. :. Crop Sci. (1980) 20, 284http://doi.org/10.2135/cropsci1980.0011183X002000020040x, 10Starling, T.M., Registration of 'Sussex' barley. :. Crop Sci. (1984) 24, 617http://doi.org/10.2135/cropsci1984.0011183X002400030045x, 11Starling, T.M., Registration of 'Wysor' barley. :. Crop Sci. (1987) 27, 1306http://doi.org/10.2135/cropsci1987.0011183X002700060047x, 12Starling, T.M., Registration of 'Nomini' barley. :. Crop Sci. (1994) 34, 300http://doi.org/10.2135/cropsci1994.0011183X003400010057x, 13Wiebe, G.A., 'Trebi' and 'Harlan' barleys. :. Crop Sci. (1965) 5, 196http://doi.org/10.2135/cropsci1965.0011183X000500020040x Citing Literature Volume45, Issue2March–April 2005Pages 792-793 ReferencesRelatedInformation}, number={2}, journal={CROP SCIENCE}, author={Brooks, WS and Vaughn, ME and Griffey, CA and Price, AM and Pridgen, TH and Rohrer, WL and Brann, DE and Rucker, EG and Behl, HD and Sisson, WL and et al.}, year={2005}, pages={792–793} }
 @article{brooks_vaughn_griffey_price_pridgen_rohrer_brann_rucker_behl_sisson_et al._2005, title={Registration of 'Price' barley}, volume={45}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2005.0791}, abstractNote={Crop ScienceVolume 45, Issue 2 p. 791-792 Registrations of Cultivars Registration of 'Price' Barley W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author First published: 01 March 2005 https://doi.org/10.2135/cropsci2005.0791Citations: 10 Registered by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1Caldwell, R.M., Registration of 'Harrison' barley. :. Crop Sci. (1966) 6, 387http://doi.org/10.2135/cropsci1966.0011183X000600040038x, 10.2135/cropsci1966.0011183X000600040037x Google Scholar 2Price, A.M., Registration of 'Callao' Barley. :. Crop Sci. (1996) 36, 1077http://doi.org/10.2135/cropsci1996.0011183X0036000400050x, 10.2135/cropsci1996.0011183X0036000400049x Google Scholar 3Starling, T.M., Registration of 'Henry' barley. :. Crop Sci. (1980) 20, 284http://doi.org/10.2135/cropsci1980.0011183X002000020040x, 10.2135/cropsci1980.0011183X002000020040x Google Scholar 4Starling, T.M., Registration of 'Wysor' barley. :. Crop Sci. (1987) 27, 1306http://doi.org/10.2135/cropsci1987.0011183X002700060047x, 10.2135/cropsci1987.0011183X002700060048x Web of Science®Google Scholar 5Starling, T.M., Registration of 'Nomini' barley. :. Crop Sci. (1994) 34, 300http://doi.org/10.2135/cropsci1994.0011183X003400010057x, 10.2135/cropsci1994.0011183X003400010056x Google Scholar 6Wiebe, G.A., 'Trebi' and 'Harlan' barleys. :. Crop Sci. (1965) 5, 196http://doi.org/10.2135/cropsci1965.0011183X000500020040x 10.2135/cropsci1965.0011183X000500020040x Google Scholar Citing Literature Volume45, Issue2March–April 2005Pages 791-792 ReferencesRelatedInformation}, number={2}, journal={CROP SCIENCE}, author={Brooks, WS and Vaughn, ME and Griffey, CA and Price, AM and Pridgen, TH and Rohrer, WL and Brann, DE and Rucker, EG and Behl, HD and Sisson, WL and et al.}, year={2005}, pages={791–792} }
 @article{brooks_vaughn_griffey_price_pridgen_rohrer_brann_rucker_behl_sisson_et al._2005, title={Registration of 'Thoroughbred' barley}, volume={45}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2005.0789}, abstractNote={Crop ScienceVolume 45, Issue 2 p. 789-790 Registrations of Cultivars Registration of 'Thoroughbred' Barley W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author W.S. Brooks, W.S. Brooks Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorM.E. Vaughn, M.E. Vaughn Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorC.A. Griffey, Corresponding Author C.A. Griffey [email protected] Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Corresponding author ([email protected])Search for more papers by this authorA.M. Price, A.M. Price Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorT.H. Pridgen, T.H. Pridgen Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Rohrer, W.L. Rohrer Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorD.E. Brann, D.E. Brann Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorE.G. Rucker, E.G. Rucker Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorH.D. Behl, H.D. Behl Crop and Soil Environmental Sciences Dep., Virginia Polytechnic Inst. and State Univ., Blacksburg, VA, 24061Search for more papers by this authorW.L. Sisson, W.L. Sisson Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.A. Corbin, R.A. Corbin Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorJ.C. Kenner, J.C. Kenner Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorD.W. Dunaway, D.W. Dunaway Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR.M. Pitman, R.M. Pitman Eastern Virginia Agricultural Research and Extension Center, Virginia Polytechnic Inst. and State Univ., Warsaw, VA, 22572Search for more papers by this authorR. Premakumar, R. Premakumar USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this authorD.P. Livingston, D.P. Livingston USDA-ARS Plant Science Unit, Department of Crop Science, North Carolina State Univ., Raleigh, NC, 27695Search for more papers by this author First published: 01 March 2005 https://doi.org/10.2135/cropsci2005.0789Citations: 12 Registered by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume45, Issue2March–April 2005Pages 789-790 RelatedInformation}, number={2}, journal={CROP SCIENCE}, author={Brooks, WS and Vaughn, ME and Griffey, CA and Price, AM and Pridgen, TH and Rohrer, WL and Brann, DE and Rucker, EG and Behl, HD and Sisson, WL and et al.}, year={2005}, pages={789–790} }
 @article{livingston_premakumar_2002, title={Apoplastic carbohydrates do not account for differences in freezing tolerance of two winter-oat cultivars that have been second phase cold-hardened}, volume={30}, number={3-4}, journal={Cereal Research Communications}, author={Livingston, D. P. and Premakumar, R.}, year={2002}, pages={375–381} }
 @article{livingston_olien_premakumar_2000, title={Thermal effect of CO2 on apoplastic ice in rye and oat during freezing}, volume={122}, ISSN={["0032-0889"]}, DOI={10.1104/pp.122.3.861}, abstractNote={Abstract}, number={3}, journal={PLANT PHYSIOLOGY}, author={Livingston, DP and Olien, CR and Premakumar, R}, year={2000}, month={Mar}, pages={861–865} }
 @article{premakumar_jacobitz_ricke_bishop_1996, title={Phenotypic characterization of a tungsten-tolerant mutant of Azotobacter vinelandii}, volume={178}, ISSN={["1098-5530"]}, DOI={10.1128/jb.178.3.691-696.1996}, abstractNote={A tungsten-tolerant mutant strain (CA6) of Azotobacter vinelandii first described in 1980 (P. E. Bishop, D. M. L. Jarlenski, and D. R. Hetherington, Proc. Natl. Acad. Sci. USA 77:7342-7346, 1980) has been further characterized. Results from growth experiments suggest that both nitrogenases 1 and 3 are utilized when CA6 grows in N-free medium containing Na2MoO4. Strain CA6.1.71, which lacks both nitrogenases 2 and 3, grew as well as strain CA in N-free medium containing Na2MoO4 after an initial lag. This indicates that nitrogenase 1 is fully functional in strain CA6. nifH-lacZ and anfH-lacZ transcriptional fusions were expressed in CA6 in the presence of Na2MoO4. Thus, in contrast to wild-type strain CA, transcription of the anfHDGK gene cluster in strain CA6 is not repressed by Mo. Expression of the vnfD-lacZ fusion was the same in both strains CA and CA6. In agreement with the results obtained with lac fusions, subunits of both nitrogenases 1 and 3 were found in protein extracts of CA6 cells grown in N-free medium containing Na2MoO4. However, CA6 cells, cultured in the presence of Na2WO4, accumulated nitrogenase 3 proteins without detectable amounts of nitrogenase 1 proteins. This indicates that expression of Mo-independent nitrogenase 3 is the basis for the tungsten tolerance phenotype of strain CA6. A measure of Mo accumulation as a function of time showed that accumulation by strain CA6 was slower than that for strain CA. When Mo accumulation was studied as a function of Na2MoO4 concentration, the two strains accumulated similar amounts of Mo in the concentration range of 0 to 1 microM Na2MoO4 during a 2-h period. Within the range of 1 to 5 microM Na2MoO4, Mo accumulation by strain CA increased linearly with increasing concentration whereas no further increases were observed for strain CA6. These results are consistent with the possibility that the tungsten tolerance mutation carried by CA6 is in a Mo transport system.}, number={3}, journal={JOURNAL OF BACTERIOLOGY}, author={Premakumar, R and Jacobitz, S and Ricke, SC and Bishop, PE}, year={1996}, month={Feb}, pages={691–696} }
 @article{premakumar_loveless_bishop_1994, title={EFFECT OF AMINO-ACID SUBSTITUTIONS IN A POTENTIAL METAL-BINDING SITE OF ANFA ON EXPRESSION FROM THE ANFH PROMOTER IN AZOTOBACTER-VINELANDII}, volume={176}, ISSN={["1098-5530"]}, DOI={10.1128/jb.176.19.6139-6142.1994}, abstractNote={AnfA, an activator required for transcription of the structural genes encoding nitrogenase 3 (anfHDGK) in Azotobacter vinelandii, has a potential metal-binding site [(S19)H(C21)FTGE(C26)R] in its N terminus. Growth studies and expression of an anfH-lacZ fusion in mutants containing amino acid substitutions in this site indicate that Ser-19 is not required for AnfA activity whereas Cys-21 and Cys-26 are required. Residual expression of the anfH-lacZ fusion in AnfA- mutants was found to be due to activation by VnfA, the activator required for expression of genes encoding nitrogenase 2.}, number={19}, journal={JOURNAL OF BACTERIOLOGY}, author={PREMAKUMAR, R and LOVELESS, TM and BISHOP, PE}, year={1994}, month={Oct}, pages={6139–6142} }
 @article{premakumar_lemos_bishop_1984, title={EVIDENCE FOR 2 DINITROGENASE REDUCTASES UNDER REGULATORY CONTROL BY MOLYBDENUM IN AZOTOBACTER-VINELANDII}, volume={797}, ISSN={["0006-3002"]}, DOI={10.1016/0304-4165(84)90382-9}, abstractNote={Evidence for an alternative nitrogen fixation system which is expressed under conditions of molybdenum deficiency has been reported in Azotobacter vinelandii (Bishop, P.E., Jarlenski, D.M.L. and Hetherington, D.R., Proc. Natl. Acad. Sci. U.S.A. (1980) 77, 7342–7346). In the present report we describe the existence of activity for a dinitrogenase reductase-like enzyme (alternative reductase) in Mo-deficient cell-free extracts of Nif− mutant strains of A. vinelandii which lack either conventional dinitrogenase reductase (strains UW1 and UW3) or contain a defective enzyme (strain UW91) under conditions of Mo-sufficiency. Nitrogenase activities were determined by the acetylene reduction method in a complementation assay where extracts of strain UW91 served as a source of dinitrogenase and extracts of strains UW1, UW3 or UW91 served as a source of alternative reductase. Strains that lack dinitrogenase reductase activity in the presence of Mo, were shown to have alternative reductase activity under Mo-deficient conditions. Two-dimensional gel electrophoretic analysis showed these extracts to contain a protein of similar mobility as the conventional dinitrogenase reductase. Molybdenum and tungsten repressed the formation of the alternative reductase whereas vanadium mimicked Mo deprivation. In conclusion, the results with the Nif− strains provide evidence for the presence of two reductase activities, one of which is expressed in the presence of Mo (dinitrogenase reductase) and the other in the absence of Mo (alternative reductase).}, number={1}, journal={BIOCHIMICA ET BIOPHYSICA ACTA}, author={PREMAKUMAR, R and LEMOS, EM and BISHOP, PE}, year={1984}, pages={64–70} }