@article{koltai_bird_2002, title={Recovery and sequence validation of the histological signal following in situ RT-PCR localization of plant gene transcripts}, volume={20}, ISSN={["0735-9640"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-4544341383&partnerID=MN8TOARS}, DOI={10.1007/BF02772126}, number={4}, journal={PLANT MOLECULAR BIOLOGY REPORTER}, author={Koltai, H and Bird, DM}, year={2002}, month={Dec}, pages={391–397} }
 @article{koltai_dhandaydham_opperman_thomas_bird_2001, title={Overlapping plant signal transduction pathways induced by a parasitic nematode and a rhizobial endosymbiont}, volume={14}, ISSN={["0894-0282"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0034800333&partnerID=MN8TOARS}, DOI={10.1094/MPMI.2001.14.10.1168}, abstractNote={Root-knot nematodes and rhizobia establish interactions with roots characterized by the de novo induction of host structures, termed giant cells and nodules, respectively. Two transcription regulators, PHAN and KNOX, required for the establishment of meristems were previously shown to be expressed in tomato giant cells. We isolated the orthologues of PHAN and KNOX (Mt-phan and Mt-knox-1) from the model legume Medicago truncatula, and established the spatial distribution of their expression in situ. We confirmed that Mt-phan and Mt-knox-1 are expressed in lateral root initials and in nematode-induced giant cells and showed that they are expressed in nodules induced by Sinorhizobium meliloti. Expression of both genes becomes spatially restricted as the nodules develop. We further examined nematode feeding sites for the expression of two genes involved in nodule formation, ccs52 (encodes a mitotic inhibitor) and ENOD40 (encodes an early, nodulation mitogen), and found transcripts of both genes to be present in and around giant cells induced in Medicago. Collectively, these results reveal common elements of host responses to mutualistic and parasitic plant endosymbionts and imply that overlapping regulatory pathways lead to giant cells and nodules. We discuss these pathways in the context of phytohormones and parallels between beneficial symbiosis and disease.}, number={10}, journal={MOLECULAR PLANT-MICROBE INTERACTIONS}, author={Koltai, H and Dhandaydham, M and Opperman, C and Thomas, J and Bird, D}, year={2001}, month={Oct}, pages={1168–1177} }
 @article{koltai_bird_2000, title={High throughput cellular localization of specific plant mRNAs by liquid-phase in situ reverse transcription-polymerase chain reaction of tissue sections}, volume={123}, ISSN={["0032-0889"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0033836945&partnerID=MN8TOARS}, DOI={10.1104/pp.123.4.1203}, abstractNote={Abstract Advances in high throughput DNA sequencing and bioinformatic gene discovery far outpace our ability to analyze gene function, necessitating development of more efficient means to examine expression at the cellular level. Here we present a polymerase chain reaction-based method to detect mRNA species in situ in which essentially all of the steps are carried out in liquid phase in a 96-well microtiter tray and only the final signal detection is performed on a microscope slide. We demonstrate the sensitivity of the method by the cellular localization of mRNA for the Tkn2transcription factor in a wide variety of plant tissues, and its selectivity in discriminating a single gene family member by the in situ localization of rbcs3 transcripts. Furthermore, we demonstrate the utility of the in-well in situ method in detectingFDL and IFL1 transcripts in Arabidopsis sections, thus establishing the method as a tool to determine spatial expression pattern of sequences obtained from genomic sequencing projects. Being amenable to robotic processing, in-well in situ reverse transcription-polymerase chain reaction permits a great enhancement in the number of tissue samples that can be processed. Consequently, this method may become a powerful tool for functional genomics studies, permitting the cellular site of transcription of large numbers of sequences obtained from databases to be rapidly established.}, number={4}, journal={PLANT PHYSIOLOGY}, author={Koltai, H and Bird, DM}, year={2000}, month={Aug}, pages={1203–1212} }
 @article{bird_koltai_2000, title={Plant parasitic nematodes: Habitats, hormones, and horizontally-acquired genes}, volume={19}, number={2}, journal={Journal of Plant Growth Regulation}, author={Bird, D. M. and Koltai, H.}, year={2000}, pages={183–194} }