Kai Koo

Works (4)

Updated: July 5th, 2023 16:01

2003 journal article

Detection of Listeria monocytogenes from a model food by fluorescence resonance energy transfer-based PCR with an asymmetric fluorogenic probe set

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(2), 1082–1088.

By: K. Koo n & L. Jaykus n

MeSH headings : Animals; Bacterial Proteins / genetics; Bacterial Toxins; Culture Media; DNA Probes; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Heat-Shock Proteins / genetics; Hemolysin Proteins; Listeria monocytogenes / growth & development; Listeria monocytogenes / isolation & purification; Milk / microbiology; Polymerase Chain Reaction; Sensitivity and Specificity
TL;DR: This study demonstrated that this asymmetric FRET-based amplification and detection protocol provides an alternative approach for endpoint detection of nucleic acid amplification products as applied to detection of pathogens in a model food. (via Semantic Scholar)
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Added: August 6, 2018

2002 journal article

Detection of single nucleotide polymorphisms within the Listeria genus using an 'asymmetric' fluorogenic probe set and fluorescence resonance energy transfer based-PCR

LETTERS IN APPLIED MICROBIOLOGY, 35(6), 513–517.

By: K. Koo n & L. Jaykus n

MeSH headings : Fluorescence Resonance Energy Transfer / methods; Fluorescent Dyes; Listeria / classification; Listeria / genetics; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide / genetics; RNA, Ribosomal, 16S / genetics; Species Specificity
TL;DR: A novel and inexpensive fluorescence energy transfer (FRET)‐based PCR protocol to distinguish single nucleotide polymorphisms (SNPs) within the genus Listeria is described. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2000 journal article

Modified method to detect PCR products by 5 ' nuclease activity and an asymmetric fluorogenic probe set

BIOTECHNIQUES, 29(4), 690-+.

By: K. Koo n & L. Jaykus n

MeSH headings : Deoxyribonucleases / metabolism; Fluorescent Dyes; Polymerase Chain Reaction / methods
TL;DR: An asymmetric fluorogenic probe set was designed and tested for its applicability to the rapid endpoint detection of PCR products and it was hypothesized that most of the reporter probe molecules are available to hybridize with the target PCR products, after which they are digested by the 5′ nuclease activity of Taq DNA polymerase. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2000 journal article

Selective amplification of bacterial RNA: use of a DNA primer containing mismatched bases near its 3 ' terminus to reduce false-positive signals

LETTERS IN APPLIED MICROBIOLOGY, 31(3), 187–192.

By: K. Koo n & L. Jaykus n

MeSH headings : 3' Untranslated Regions; Bacillus cereus / genetics; Bacillus cereus / growth & development; Bacterial Toxins; Base Pair Mismatch / genetics; DNA Primers / genetics; False Positive Reactions; Heat-Shock Proteins / genetics; Hemolysin Proteins; Listeria monocytogenes; Nucleic Acid Amplification Techniques / methods; RNA, Bacterial / genetics; RNA, Bacterial / isolation & purification; RNA, Messenger / genetics; RNA, Ribosomal, 16S / genetics; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The RT–PCR method was readily adapted to the selective amplification of mRNA of the Listeria monocytogenes listeriolysin O (hly) gene, and amplification of genomic DNA was hindered relative to the amplification of cDNA. (via Semantic Scholar)
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Added: August 6, 2018

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