@article{o'rourke_pitulle_hegarty_kraycirik_killary_grosenstein_brown_breitschwerdt_2005, title={Bartonella quintana in Cynomolgus Monkey (Macaca fascicularis)}, volume={11}, ISSN={1080-6040 1080-6059}, url={http://dx.doi.org/10.3201/eid1112.030045}, DOI={10.3201/eid1112.030045}, abstractNote={We identified a Bartonella quintana strain by polymerase chain reaction amplification, cloning, and sequencing of DNA extracted from lysed erythrocytes and cultured colonies grown from peripheral blood collected from a captive-bred cynomolgus monkey (Macaca fascicularis). This report describes naturally acquired B. quintana infection in a nonhuman primate.}, number={12}, journal={Emerging Infectious Diseases}, publisher={Centers for Disease Control and Prevention (CDC)}, author={O'Rourke, Laurie G. and Pitulle, Christian and Hegarty, Barbara C. and Kraycirik, Sharon and Killary, Karen A. and Grosenstein, Paul and Brown, James W. and Breitschwerdt, Edward B.}, year={2005}, month={Dec}, pages={1931–1934} }
 @article{frey_pressler_guy_pitulle_breitschwerdt_2003, title={Capnocytophaga sp. Isolated from a Cat with Chronic Sinusitis and Rhinitis}, volume={41}, ISSN={0095-1137}, url={http://dx.doi.org/10.1128/jcm.41.11.5321-5324.2003}, DOI={10.1128/JCM.41.11.5321-5324.2003}, abstractNote={ABSTRACT
          
            A
            Capnocytophaga
            sp. was inadvertently isolated from a cat with chronic sinusitis and rhinitis when cytopathic effects were observed in Crandall-Reese feline kidney cells that had been inoculated with oropharyngeal swab samples. Although
            Capnocytophaga
            spp. are of considerable zoonotic importance, their clinical relevance for dogs or cats has not been established. However, failure to do so may be attributed to the infrequent use of specialized isolation techniques that are required to grow
            Capnocytophaga
            spp. To our knowledge, successful isolation of these organisms from a cat with nasopharyngeal disease has not been reported.
          }, number={11}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Frey, E. and Pressler, B. and Guy, J. and Pitulle, C. and Breitschwerdt, E.}, year={2003}, month={Nov}, pages={5321–5324} }
 @article{birkenheuer_breitschwerdt_alleman_pitulle_2002, title={Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences}, volume={63}, ISSN={0002-9645}, url={http://dx.doi.org/10.2460/ajvr.2002.63.1385}, DOI={10.2460/ajvr.2002.63.1385}, abstractNote={AbstractObjective—To determine whetherHaemobartonella canisandMycoplasma haemofelis(formerly known asH felis[large form]) can be differentiated by use of comparative analysis of gene sequences.Sample Population—Blood samples obtained from 3 dogs infected withH canisand 2 cats infected withM haemofelis.Procedure—The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained fromH canis-infected dogs andM haemofelis-infected cats. The DNA sequences were subjected to comparative analysis.Results—The 16S rDNA sequences ofH canisandM haemofeliswere nearly identical (homology of 99.3 to 99.7%). In contrast, RNase P gene sequences had a lower degree of sequence homology between the 2 organisms (94.3 to 95.5%).Conclusions and Clinical Relevance—Haemobartonella canisandM haemofelisare not identical organisms. Molecular differentiation ofH canisandM haemofelisis more clearly evident by use of comparative analysis of RNase P gene sequences than by comparative analysis of 16S rDNA gene sequences. (Am J Vet Res2002;63:1385–1388)}, number={10}, journal={American Journal of Veterinary Research}, publisher={American Veterinary Medical Association (AVMA)}, author={Birkenheuer, Adam J. and Breitschwerdt, Edward B. and Alleman, A. Rick and Pitulle, Christian}, year={2002}, month={Oct}, pages={1385–1388} }
 @article{pitulle_strehse_brown_breitschwerdt_2002, title={Investigation of the phylogenetic relationships within the genus Bartonella based on comparative sequence analysis of the rnpB gene, 16S rDNA and 23S rDNA}, volume={52}, ISSN={["1466-5034"]}, DOI={10.1099/ijs.0.02281-0}, number={2002 Nov}, journal={INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, author={Pitulle, C and Strehse, C and Brown, JW and Breitschwerdt, EB}, year={2002}, month={Nov}, pages={2075–2080} }
 @article{pressler_hardie_pitulle_hopwood_sontakke_breitschwerdt_2002, title={Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion}, volume={220}, ISSN={0003-1488}, url={http://dx.doi.org/10.2460/javma.2002.220.1336}, DOI={10.2460/javma.2002.220.1336}, abstractNote={A 3-year-old spayed female Whippet was examined for cough and respiratory distress. Lung lobe torsion with pleural effusion was diagnosed, and lung lobectomy was performed. Pleural effusion recurred during the following 27 months; conventional bacteriologic cultures of pleural effusion did not result in bacterial growth. A second lung lobectomy, pleuroperitoneal shunt placement. and pericardectomy were subsequently performed. Mycobacterium kansasii was eventually isolated from pleural fluid and identified by polymerase chain reaction amplification and DNA sequencing. The dog was euthanatized before therapeutic response could be evaluated. To our knowledge, this is the first report of M. kansasii infection in a dog. Additionally, this is the first report of mycobacterial isolation from pleural fluid, and one of few reports of antemortem mycobacterial isolation from a body fluid, as opposed to identification in specimens during histologic examination. Routine bacteriologic culture methods are insufficient to isolate mycobacterial agents, and special methods are indicated in dogs with persistent pleural effusion.}, number={9}, journal={Journal of the American Veterinary Medical Association}, publisher={American Veterinary Medical Association (AVMA)}, author={Pressler, Barrak M. and Hardie, Elizabeth M. and Pitulle, Christian and Hopwood, Robin M. and Sontakke, Sushama and Breitschwerdt, Edward B.}, year={2002}, month={May}, pages={1336–1340} }
 @article{suksawat_pitulle_arraga-alvarado_madrigal_hancock_breitschwerdt_2001, title={Coinfection with Three Ehrlichia Species in Dogs from Thailand and Venezuela with Emphasis on Consideration of 16S Ribosomal DNA Secondary Structure}, volume={39}, ISSN={0095-1137}, url={http://dx.doi.org/10.1128/JCM.39.1.90-93.2001}, DOI={10.1128/JCM.39.1.90-93.2001}, abstractNote={ABSTRACT
          
            As part of a larger study to investigate tick-borne infections in dogs from Thailand and Venezuela, documentation of coinfection with three
            Ehrlichia
            species in two dogs, one from each country, became the focus of the present study. Although neither dog had clinical signs attributable to ehrlichiosis, both dogs were anemic and neutropenic and the Thai dog was thrombocytopenic. Genus- and species-specific PCR targeting the 16S rRNA genes indicated that both dogs were coinfected with
            Ehrlichia canis
            ,
            E. platys
            , and
            E. equi
            . To our knowledge, these results provide the first molecular documentation for the presence of
            E. equi
            in dogs from these countries. Using universal bacterial PCR primers, one nearly full-length 16S rRNA gene could be amplified from each dog. The sequences were identical to each other and almost identical to that of
            E. platys
            (
            AF156784
            ), providing the first
            E. platys
            16S ribosomal DNA (rDNA) sequences reported from these two geographically divergent countries. To determine whether these sequence differences allow differentiation between these two strains and other published 16S rDNA
            E. platys
            sequences, we performed a phylogenetic analysis of the rRNA, incorporating the consideration of secondary structure.
          }, number={1}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Suksawat, J. and Pitulle, C. and Arraga-Alvarado, C. and Madrigal, K. and Hancock, S. I. and Breitschwerdt, E. B.}, year={2001}, month={Jan}, pages={90–93} }
 @article{hancock_breitschwerdt_pitulle_2001, title={Differentiation of Ehrlichia platys and E. equi Infections in Dogs by Using 16S Ribosomal DNA-Based PCR}, volume={39}, ISSN={0095-1137}, url={http://dx.doi.org/10.1128/JCM.39.12.4577-4578.2001}, DOI={10.1128/JCM.39.12.4577-4578.2001}, abstractNote={ABSTRACT
          
            We have encountered a previously unrecognized specificity problem when using the small-subunit ribosomal DNA (16S rDNA)-based PCR primers recommended for use in the identification of
            Ehrlichia equi
            in clinical samples. These PCR primers annealed to
            E. platys
            16S rDNA in blood samples containing high levels of
            E. platys
            organisms. Therefore, we designed and tested new PCR primers for the identification of
            E. equi
            .
          }, number={12}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Hancock, S. I. and Breitschwerdt, E. B. and Pitulle, C.}, year={2001}, month={Dec}, pages={4577–4578} }
 @article{frank_adami_ehringer_pitulle_pace_2000, title={Phylogenetic-comparative analysis of the eukaryal ribonuclease P RNA}, volume={6}, ISSN={["1469-9001"]}, DOI={10.1017/S1355838200001461}, abstractNote={Ribonuclease P (RNase P) is the ribonucleoprotein enzyme that cleaves 5'-leader sequences from precursor-tRNAs. Bacterial and eukaryal RNase P RNAs differ fundamentally in that the former, but not the latter, are capable of catalyzing pre-tRNA maturation in vitro in the absence of proteins. An explanation of these functional differences will be assisted by a detailed comparison of bacterial and eukaryal RNase P RNA structures. However, the structures of eukaryal RNase P RNAs remain poorly characterized, compared to their bacterial and archaeal homologs. Hence, we have taken a phylogenetic-comparative approach to refine the secondary structures of eukaryal RNase P RNAs. To this end, 20 new RNase P RNA sequences have been determined from species of ascomycetous fungi representative of the genera Arxiozyma, Clavispora, Kluyveromyces, Pichia, Saccharomyces, Saccharomycopsis, Torulaspora, Wickerhamia, and Zygosaccharomyces. Phylogenetic-comparative analysis of these and other sequences refines previous eukaryal RNase P RNA secondary structure models. Patterns of sequence conservation and length variation refine the minimum-consensus model of the core eukaryal RNA structure. In comparison to bacterial RNase P RNAs, the eukaryal homologs lack RNA structural elements thought to be critical for both substrate binding and catalysis. Nonetheless, the eukaryal RNA retains the main features of the catalytic core of the bacterial RNase P. This indicates that the eukaryal RNA remains intrinsically a ribozyme.}, number={12}, journal={RNA}, author={Frank, DN and Adami, C and Ehringer, MA and Pitulle, C and Pace, NR}, year={2000}, month={Dec}, pages={1895–1904} }