Marybeth Anne Pysz

Works (6)

Updated: July 5th, 2023 15:59

2005 journal article

Genorne-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga maritima

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(9), 5572–5576.

By: K. Shockley n, K. Scott *, M. Pysz n, S. Conners n, M. Johnson n, C. Montero n, R. Wolfinger*, R. Kelly n 

TL;DR: Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 article

Functional genomics-based studies of the microbial ecology of hyperthermophilic micro-organisms

Johnson, M. R., Montero, C. I., Conners, S. B., Shockley, K. R., Pysz, M. A., & Kelly, R. M. (2004, April). BIOCHEMICAL SOCIETY TRANSACTIONS, Vol. 32, pp. 188–192.

By: M. Johnson n, C. Montero n, S. Conners n, K. Shockley n, M. Pysz n & R. Kelly n 

author keywords: ecology; functional qenomics; hyperthermophile; microarray; mixed culture
MeSH headings : Biofilms; DNA, Complementary / metabolism; Ecology; Gene Transfer Techniques; Genes, Archaeal; Genome; Genome, Archaeal; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Phylogeny; RNA, Ribosomal, 16S / genetics; Temperature
TL;DR: Functional genomics-based approaches are described, using cDNA microarrays, to gain insight into how ecological features such as biofilm formation, species interaction, and possibly even gene transfer may occur in native environments, as well as to determine what genes or sets of genes may be tied to environmental functionality. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 review

Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima

[Review of ]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(10), 6098–6112.

By: M. Pysz n, S. Conners n, C. Montero n, K. Shockley n, M. Johnson n, D. Ward n, R. Kelly n 

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Base Sequence; Biofilms / growth & development; Bioreactors; DNA, Bacterial / genetics; Gene Expression Profiling; Genes, Bacterial; Iron / metabolism; Models, Biological; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Polysaccharides, Bacterial / genetics; Polysaccharides, Bacterial / metabolism; Sulfur / metabolism; Temperature; Thermotoga maritima / genetics; Thermotoga maritima / physiology; Thermotoga maritima / ultrastructure; Transcription, Genetic
TL;DR: In addition to revealing insights into the phenotype of sessile T. maritima communities, the methodology developed here can be extended to study other anaerobic biofilm formation processes as well as to examine aspects of microbial ecology in hydrothermal environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Transcriptional analysis of dynamic heat-shock response by the hyperthermophilic bacterium Thermotoga maritima

EXTREMOPHILES, 8(3), 209–217.

By: M. Pysz n, D. Ward n, K. Shockley n, C. Montero n, S. Conners n, M. Johnson n, R. Kelly n 

author keywords: cDNA microarray; groESL; heat shock; hrcA; hyperthermophile; Thermotoga
MeSH headings : Base Sequence; DNA, Bacterial / genetics; Endopeptidases / genetics; Gene Expression; Genome, Bacterial; Heat-Shock Response / genetics; Hot Temperature; Oligonucleotide Array Sequence Analysis; SOS Response, Genetics; Sigma Factor / genetics; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR: The results reported here indicate that, while T. maritima shares core elements of the bacterial heat-shock response with mesophiles, the thermal stress regulatory strategies of this organism differ significantly, and it remains to be elucidated whether these differences are related to thermophilicity or phylogenetic placement. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Growth of hyperthermophilic Archaeon Pyrococcus futiosus on chitin involves two family 18 chitinases

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(6), 3119–3128.

By: J. Gao  n, M. Bauer n, K. Shockley n, M. Pysz n & R. Kelly n 

MeSH headings : Amino Acid Sequence; Calorimetry; Chitin / metabolism; Chitinases / chemistry; Chitinases / classification; Chitinases / genetics; Chitinases / metabolism; Culture Media; Hexosaminidases / chemistry; Hexosaminidases / genetics; Hexosaminidases / metabolism; Hot Temperature; Molecular Sequence Data; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Recombinant Proteins / metabolism; Sequence Analysis, DNA
TL;DR: Two open reading frames (chiA and chiB) were identified in the genome of P. furiosus, which encodes chitinases with sequence similarity to proteins from the glycosyl hydrolase family 18 in less-thermophilic organisms, indicating that these two enzymes work together to recruit chit in-based substrates for P.furiosus growth. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Rheology of protein gels synthesized through a combined enzymatic and heat treatment method

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 31(1-3), 37–44.

By: M. Burke n, S. Ha n, M. Pysz n & S. Khan n 

author keywords: whey protein; rheology; gels; enzyme; low pH
MeSH headings : Dose-Response Relationship, Drug; Enzymes / chemistry; Hot Temperature; Hydrogen-Ion Concentration; Plant Proteins / chemistry; Proteins / chemistry; Temperature; Time Factors; Transaminases / chemistry
TL;DR: Dynamic rheological measurements reveal that the gel elastic modulus and yield stress increase substantially when heat treatment is supplemented with enzyme treatment, which leads to two types of gel microstructure at short and long enzyme treatment times, with the extent of enzyme diffusion modulating the structure at intermediate times. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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