Works (2)
Updated: July 5th, 2023 16:01
2002 article
Immobilization and Utilization of the Recombinant Fusion Proteins Trypsin−Streptavidin and Streptavidin−Transglutaminase for Modification of Whey Protein Isolate Functionality
Wilcox, C. P., Clare, D. A., Valentine, V. W., & Swaisgood, H. E. (2002, May 21). Journal of Agricultural and Food Chemistry.
author keywords: immobilization; trypsin; transglutaminase; whey protein; functionality
MeSH headings : Adsorption; Biotinylation; Chemical Phenomena; Chemistry, Physical; Cross-Linking Reagents; DNA, Recombinant; Dithiothreitol / pharmacology; Enzymes, Immobilized; Escherichia coli / genetics; Gels; Glass; Milk Proteins / chemistry; Milk Proteins / metabolism; Recombinant Fusion Proteins; Streptavidin / genetics; Temperature; Transglutaminases / genetics; Transglutaminases / metabolism; Trypsin / genetics; Trypsin / metabolism; Viscosity; Whey Proteins
topics (OpenAlex): Proteins in Food Systems; Blood properties and coagulation; Transgenic Plants and Applications
TL;DR:
By combining limited proteolysis with controlled cross-linking, it was possible to create derivatives of whey proteins with enhanced functional properties that allowed for recycling of the enzyme, eliminated the requirement for a downstream inactivation step, and permitted control over the extent of modification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 article
Molecular design, expression, and affinity immobilization of a trypsin-streptavidin fusion protein☆
Clare, D. A., Valentine, V. W., Catignani, G. L., & Swaisgood, H. E. (2001, April 1). Enzyme and Microbial Technology.
author keywords: cloning; protein expression; immobilized enzymes; trypsin
topics (OpenAlex): Biotin and Related Studies; Bacterial Genetics and Biotechnology; Advanced biosensing and bioanalysis techniques
TL;DR:
This enzyme bioreactor should serve as an excellent prototype for future studies that will examine the effect of limited proteolysis on functional characteristics of milk proteins, including gelling, emulsifying and foaming properties.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018