Works (4)

Updated: July 5th, 2023 16:00

2007 journal article

Modification of Lactobacillus beta-glucuronidase activity by random mutagenesis

GENE, 389(2), 122–127.

By: M. Callanan n, W. Russell n & T. Klaenhammer n

author keywords: Lactobacillus gasseri gusA; gene; reporter enzyme; pH range; Epicurian coli
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; DNA, Bacterial; Escherichia coli / enzymology; Escherichia coli / genetics; Genes, Bacterial; Glucuronidase / chemistry; Glucuronidase / genetics; Glucuronidase / metabolism; Hydrogen-Ion Concentration; Lactobacillus / enzymology; Lactobacillus / genetics; Molecular Sequence Data; Mutagenesis; Sequence Alignment; Sequence Analysis, DNA; Transformation, Bacterial
TL;DR: The modified GusA3 enzyme has expanded potential for use as a reporter enzyme in expression hosts that are not acidophilic, as well as lactic acid bacteria and other microorganisms that grow in acidifying environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM


By: E. Altermann n, W. Russell n, M. Azcarate-Peril n, R. Barrangou n, B. Buck n, O. McAuliffe n, N. Souther n, A. Dobson n ...

author keywords: adhesion; stress response; proteolytic system; sugar metabolism; in silico analysis
MeSH headings : Bacterial Adhesion / genetics; Bacteriocins / genetics; Bacteriophages / genetics; Bacteriophages / pathogenicity; Carbohydrate Metabolism; Computational Biology; Genome, Bacterial; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Lactobacillus acidophilus / virology; Molecular Sequence Data; Peptide Hydrolases / genetics; Prophages / genetics; Sequence Analysis, DNA; Transposases / genetics
TL;DR: These features within the genome sequence of L. acidophilus are likely to contribute to the organisms' gastric survival and promote interactions with the intestinal mucosa and microbiota. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2002 journal article

Overexpression of peptidases in Lactococcus and evaluation of their release from leaky cells

JOURNAL OF DAIRY SCIENCE, 85(10), 2438–2450.

By: T. Tuler n, M. Callanan n & T. Klaenhammer n

author keywords: leaky Lactococcus; peptidase
MeSH headings : Aminopeptidases / genetics; Aminopeptidases / metabolism; Bacterial Proteins; Cloning, Molecular; Coumarins / metabolism; DNA Restriction Enzymes; Escherichia coli / genetics; Gene Expression; Genetic Vectors; Glutamyl Aminopeptidase; Lactococcus / enzymology; Lactococcus / genetics; Lactococcus lactis / enzymology; Lactococcus lactis / genetics; Metalloendopeptidases / genetics; Metalloendopeptidases / metabolism; Oligopeptides / metabolism; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Plasmids / genetics; Promoter Regions, Genetic; Substrate Specificity; Transformation, Bacterial
TL;DR: Results showed different levels of enzymatic overexpression associated with the cellular fraction and negligible amounts of activity present within the supernatant fraction of leaky lactococcal peptidases investigated in this study. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis

GENE, 278(1-2), 101–106.

By: M. Callanan*, . PW O'Toole, M. Lubbers & K. Polzin

author keywords: bacteriophage; Lactococcus; origin of replication; theta replication
MeSH headings : Bacteriophages / genetics; Base Composition; Blotting, Southern; DNA Replication; DNA, Viral / genetics; Electrophoresis, Agar Gel; Electrophoresis, Gel, Two-Dimensional; Genome, Viral; Lactococcus lactis / virology; Replication Origin / genetics
TL;DR: 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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