@article{veleva_heath_cooper_patterson_2008, title={Selective endothelial cell attachment to peptide-modified terpolymers}, volume={29}, ISSN={["1878-5905"]}, DOI={10.1016/j.biomaterials.2008.05.022}, abstractNote={In a previous report we screened a combinatorial peptide library to identify novel ligands that bind with high affinity and specificity to human blood outgrowth endothelial cells (HBOEC). In this study we demonstrate the use of the phage display-selected-HBOEC-specific peptides as a tool to direct and modulate endothelial cell (EC) behavior with a focus on designing functional biomaterials intended for use in cardiovascular applications. First, we ensured that our peptide ligands did not interfere with EC function as tested by proliferation, migration, tube formation, and response to vascular endothelial growth factor. Second, peptides that supported EC function were incorporated into methacrylic terpolymers via chain transfer free radical polymerization. The HBOEC-specific peptide, TPSLEQRTVYAK, when covalently coupled to a terpolymer matrix, retained binding affinity towards HBOEC in a serum-free medium. Under the same binding conditions, the attachment of human umbilical vein endothelial cells (HUVEC) was limited, thus establishing HBOEC specificity. To our knowledge, this is the first report demonstrating specificity in binding to peptide-modified biomaterials of mature EC, i.e., HUVEC, and EC of progenitor origin such as HBOEC. The findings from this work could facilitate the development of autologous cell therapies with which to treat cardiovascular disease.}, number={27}, journal={BIOMATERIALS}, author={Veleva, Anka N. and Heath, Daniel E. and Cooper, Stuart L. and Patterson, Cam}, year={2008}, month={Sep}, pages={3656–3661} }
 @article{veleva_cooper_patterson_2007, title={Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells}, volume={98}, ISSN={["1097-0290"]}, DOI={10.1002/bit.21420}, abstractNote={Abstract}, number={1}, journal={BIOTECHNOLOGY AND BIOENGINEERING}, author={Veleva, Anka N. and Cooper, Stuart L. and Patterson, Cam}, year={2007}, month={Sep}, pages={306–312} }
 @article{fussell_cooper_2004, title={Synthesis and characterization of acrylic terpolymers with RGD peptides for biomedical applications}, volume={25}, ISSN={["1878-5905"]}, DOI={10.1016/j.biomaterials.2003.09.062}, abstractNote={The goal of this research was to design a biomaterial, using acrylic terpolymers, which could support endothelial cells and function in small diameter vascular graft applications. Hexyl methacrylate (HMA) and octyl methacrylate (OMA) were used as comonomers to produce a material with a low glass transition temperature (T(g)). Methacrylic acid (MAA) was used to provide ionic character, and methyl methacrylate (MMA) was selected because of its wide usage in biomedical applications. Cation neutralization was employed to modify the mechanical properties. RGD-based peptide sequences were attached to promote endothelial cell adhesion, because vascular grafts seeded with endothelial cells have fewer problems with thrombosis. The two methods used to incorporate peptide sequences were a chain transfer reaction during polymerization, and a coupling reaction attaching the peptides to carboxyl groups on the polymer after polymerization. The compositions that produced T(g)s of approximately 0 degrees C were 75 mol% OMA and 92 mol% HMA. The Young's modulus of the HMA copolymer was approximately 0.37 MPa, well below the desired value of 0.9 MPa. Likewise, the Young's modulus of approximately 0.50 MPa for the OMA copolymer was also below the desired value. After partial neutralization with sodium cations, the Young's moduli increased to approximately 0.93 and 0.99 MPa, respectively. The chain transfer reaction lowered the molecular weights and mechanical properties of the copolymers, while the coupling reaction method had little effect on these properties. The chain transfer method appears to be a promising one-step method to produce polymers with a wide range of peptide concentrations.}, number={15}, journal={BIOMATERIALS}, author={Fussell, GW and Cooper, SL}, year={2004}, month={Jul}, pages={2971–2978} }
 @article{ma_cooper_2002, title={Effect of polydispersity on viscoelasticity and shear thickening in aqueous solutions of hydrocarbon end-capped poly(ethylene oxide)}, volume={35}, ISSN={["1520-5835"]}, DOI={10.1021/ma010390p}, abstractNote={The linear viscoelastic and shear thickening behavior in aqueous solutions of model hydrophobically end-capped poly(ethylene oxide) were examined. Comparisons are made between monodisperse samples, unimodal polydisperse samples, and bimodal polydisperse samples. While the linear viscoelastic behavior is qualitatively similar for both polydisperse and monodisperse systems, the steady shear response displayed a marked dependence on the type of polydispersity, especially in the shear thickening behavior. No significant shear thickening was observed in unimodal polydisperse samples, and one possible explanation for this lack of significant shear thickening could be due to a lack of cooperative non-Gaussian chain stretching. 1 Samples with bimodal molecular weight distribution exhibit similar shear thickening behavior as monodisperse samples. Furthermore, the characteristics of shear thickening of the bimodal system were found to be the same as in the monodisperse polymer with the lower molecular weight (10K), instead of that of the monodisperse polymer with the same average molecular weight (20K). The mechanism of shear thickening is due to the non-Gaussian chain stretching of the shorter polymer chain.}, number={6}, journal={MACROMOLECULES}, author={Ma, SX and Cooper, SL}, year={2002}, month={Mar}, pages={2024–2029} }
 @article{chen_cooper_2002, title={Interactions between dendrimer biocides and bacterial membranes}, volume={23}, ISSN={["1878-5905"]}, DOI={10.1016/S0142-9612(02)00036-4}, abstractNote={Dendrimer biocides have been shown to be more potent than their small molecule counterparts. In this study, several techniques were utilized to investigate the interactions between quaternary ammonium functionalized poly(propylene imine) dendrimers and bacterial membranes. Both Gram-positive and Gram-negative bacteria were tested. The techniques employed include UV-Vis spectroscopy, differential scanning calorimetry, and bioluminescence. When treated with dendrimer biocides, release of 260nm adsorbing materials from Escherichia coli strains quickly increased and reached a plateau afterwards, while release of 260 nm absorbing materials from Staphylococcus aureus increased monotonically with the concentration due to the difference in cell structures. The different release behavior also correlates with the antimicrobial properties against these two types of bacteria. Bioluminescence experiments using bacteria containing stress-responsive bioluminescent reporter gene fusions provided information suggesting that damage to the cell membranes is the primary mechanism of the antimicrobial action for dendrimer biocides. High concentrations of calcium ions can limit the efficacy of the dendrimer biocides, although the tested concentration range is much higher than most practical applications. Differential scanning calorimetry studies showed at high concentrations that dendrimer biocides formed precipitates with phospholipid vesicles, suggesting a strong interaction with this model of bacterial membrane. These results provide insight about the antibacterial action of dendrimer biocides and establish the basis for their mode of action.}, number={16}, journal={BIOMATERIALS}, author={Chen, CZS and Cooper, SL}, year={2002}, month={Aug}, pages={3359–3368} }
 @article{tegoulia_cooper_2002, title={Staphylococcus aureus adhesion to self-assembled monolayers: effect of surface chemistry and fibrinogen presence}, volume={24}, number={3-4}, journal={Colloids and Surfaces. B, Biointerfaces}, author={Tegoulia, V. A. and Cooper, S. L.}, year={2002}, pages={217–228} }