@article{diniz_wood_maggi_sontakke_stepnik_breitschwerdt_2009, title={Co-isolation of Bartonella henselae and Bartonella vinsonii subsp. berkhoffii from blood, joint and subcutaneous seroma fluids from two naturally infected dogs}, volume={138}, ISSN={0378-1135}, url={http://dx.doi.org/10.1016/j.vetmic.2009.01.038}, DOI={10.1016/j.vetmic.2009.01.038}, abstractNote={This report describes the clinical presentation, isolation and treatment of two dogs naturally infected with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii. Chronic and progressive polyarthritis was the primary complaint for dog #1, from which B. henselae and B. vinsonii subsp. berkhoffii were cultured on three independent occasions from blood and joint fluid samples, despite administration of nearly 4 months of non-consecutive antibiotic therapy. A clinically atypical and progressively severe trauma-associated seroma was the primary complaint for dog #2, from which B. henselae and B. vinsonii subsp. berkhoffii were isolated from serum, blood and seroma fluid. Dogs can be co-infected with two Bartonella spp. and infection with these organisms should not be ruled out if specific antibodies are not detected. Specialized culture techniques should be used for isolation and to assess antibiotic efficacy.}, number={3-4}, journal={Veterinary Microbiology}, publisher={Elsevier BV}, author={Diniz, Pedro Paulo Vissotto de Paiva and Wood, Michael and Maggi, Ricardo G. and Sontakke, Sushama and Stepnik, Matt and Breitschwerdt, Edward B.}, year={2009}, month={Sep}, pages={368–372} }
 @article{cadenas_maggi_diniz_breitschwerdt_sontakke_breithschwerdt_2007, title={Identification of bacteria from clinical samples using Bartonella alpha-Proteobacteria growth medium}, volume={71}, ISSN={0167-7012}, url={http://dx.doi.org/10.1016/j.mimet.2007.08.006}, DOI={10.1016/j.mimet.2007.08.006}, abstractNote={In an effort to overcome historical problems associated with the isolation of Bartonella species from animal and human blood samples, our laboratory developed a novel, chemically modified, insect-based, liquid culture medium (Bartonella alpha-Proteobacteria growth medium, BAPGM). In this study, we describe the isolation of non-Bartonella bacteria from aseptically obtained human blood and tissue samples that were inoculated into BAPGM pre-enrichment culture medium, and were obtained during attempts to define each individuals Bartonella infection status. After incubation for at least 7 days in liquid BAPGM, pre-enriched inoculums were sub-cultured onto a BAPGM/blood agar plate. Bacterial DNA was extracted from pooled plated colonies and amplified using conventional PCR targeting the 16S rRNA gene. Subsequently, amplicons were cloned, sequenced and compared to GenBank database sequences using the BLAST program. Regardless of the patient's Bartonella status, seventeen samples generated only one 16S rDNA sequence, representing the following genera: Arthrobacter, Bacillus, Bartonella, Dermabacter, Methylobacterium, Propionibacterium, Pseudomonas, Staphylococcus and bacteria listed as “non-cultured” in the GenBank database. Alkalibacterium, Arthrobacter, Erwinia, Kineococcus, Methylobacterium, Propionibacterium, Sphingomonas, and Staphylococcus were isolated from nine Bartonella-infected individuals. Co-isolation of Acinetobacter, Sphingomonas, Staphylococcus spp. and bacteria listed as “non-cultured” in the GenBank database was achieved for four samples in which Bartonella spp. were not detected. Despite the phylogenetic limitations of using partial 16S rRNA gene sequencing for species and strain identification, the investigational methodology described in this study may provide a complementary approach for the isolation and identification of bacteria from patient samples.}, number={2}, journal={Journal of Microbiological Methods}, publisher={Elsevier BV}, author={Cadenas, Maria B. and Maggi, Ricardo G. and Diniz, Pedro P.V.P. and Breitschwerdt, Kyle T. and Sontakke, Sushama and Breithschwerdt, Edward B.}, year={2007}, month={Nov}, pages={147–155} }
 @article{jones_valenzisi_sontakke_sprayberry_maggi_hegarty_breitschwerdt_2007, title={Use of an insect cell culture growth medium to isolate bacteria from horses with effusive, fibrinous pericarditis: A preliminary study}, volume={121}, ISSN={0378-1135}, url={http://dx.doi.org/10.1016/j.vetmic.2006.11.024}, DOI={10.1016/j.vetmic.2006.11.024}, abstractNote={Effusive, fibrinous pericarditis is an uncommon disease entity in horses. In 2001, pericarditis occurred in conjunction with an epizootic in central Kentucky that was associated with exposure to eastern tent caterpillars (ETCs). Bacterial isolation from equine pericardial fluid samples was attempted using an insect cell culture growth medium (ICCGM). Using previously cultured, stored frozen samples from four horses with fibrinous pericarditis, inoculation of 10% blood agar plates yielded no growth, whereas simultaneous inoculation of ICCGM resulted in the isolation of Proprionibacterium acnes, Staphylococcus equorum, a Streptococcus sp. and Pseudomonas rhodesiae from pericardial fluid samples. A similar or novel caterpillar-associated bacteria was not identified; however, use of an ICCGM might enhance isolation of bacteria from equine pericardial fluid.}, number={1-2}, journal={Veterinary Microbiology}, publisher={Elsevier BV}, author={Jones, Samuel L. and Valenzisi, Amy and Sontakke, Sushama and Sprayberry, Kimberly A. and Maggi, Ricardo and Hegarty, Barbara and Breitschwerdt, Edward}, year={2007}, month={Mar}, pages={177–181} }
 @article{kelly_rolain_maggi_sontakke_keene_hunter_lepidi_breitschwerdt_breitschwerdt_raoult_et al._2006, title={Bartonella quintana Endocarditis in Dogs}, volume={12}, ISSN={1080-6040}, url={http://dx.doi.org/10.3201/eid1212.060724}, DOI={10.3201/eid1212.060724}, abstractNote={TOC summary line: PCR and sequencing provide the first evidence that B. quintana can be pathogenic in dogs.}, number={12}, journal={Emerging Infectious Diseases}, publisher={Centers for Disease Control and Prevention (CDC)}, author={Kelly, P. and Rolain, J. M. and Maggi, Ricardo and Sontakke, S. and Keene, B. and Hunter, S. and Lepidi, H. and Breitschwerdt, K. T. and Breitschwerdt, Edward and Raoult, D. and et al.}, year={2006}, pages={1869–1872} }
 @misc{breitschwerdt_sontakke_2006, title={Media and methods for cultivation of microorganisms}, volume={7,115,385}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Breitschwerdt, E. B. and Sontakke, S.}, year={2006} }
 @article{pressler_hardie_pitulle_hopwood_sontakke_breitschwerdt_2002, title={Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion}, volume={220}, ISSN={0003-1488}, url={http://dx.doi.org/10.2460/javma.2002.220.1336}, DOI={10.2460/javma.2002.220.1336}, abstractNote={A 3-year-old spayed female Whippet was examined for cough and respiratory distress. Lung lobe torsion with pleural effusion was diagnosed, and lung lobectomy was performed. Pleural effusion recurred during the following 27 months; conventional bacteriologic cultures of pleural effusion did not result in bacterial growth. A second lung lobectomy, pleuroperitoneal shunt placement. and pericardectomy were subsequently performed. Mycobacterium kansasii was eventually isolated from pleural fluid and identified by polymerase chain reaction amplification and DNA sequencing. The dog was euthanatized before therapeutic response could be evaluated. To our knowledge, this is the first report of M. kansasii infection in a dog. Additionally, this is the first report of mycobacterial isolation from pleural fluid, and one of few reports of antemortem mycobacterial isolation from a body fluid, as opposed to identification in specimens during histologic examination. Routine bacteriologic culture methods are insufficient to isolate mycobacterial agents, and special methods are indicated in dogs with persistent pleural effusion.}, number={9}, journal={Journal of the American Veterinary Medical Association}, publisher={American Veterinary Medical Association (AVMA)}, author={Pressler, Barrak M. and Hardie, Elizabeth M. and Pitulle, Christian and Hopwood, Robin M. and Sontakke, Sushama and Breitschwerdt, Edward B.}, year={2002}, month={May}, pages={1336–1340} }