@article{upchurch_rose_eweida_zuo_2005, title={Expression of the cercosporin transporter, CFP, in tobacco reduces frog-eye lesion size}, volume={27}, ISSN={["1573-6776"]}, DOI={10.1007/s10529-005-1780-3}, abstractNote={The cercosporin Major Facilitator Superfamily (MFS) transporter, CFP, under the control of the CaMV 35S promoter, was introduced into the Xanthi cultivar of tobacco by Agrobacterium-mediated transformation. CFP(+) transgenic plants were physically indistinguishable from non-transgenic Xanthi and progressed normally through growth to seed set. Accumulation of CFP in the leaf membrane fraction of CFP(+ )transgenic plants was associated with decreased cercosporin phytotoxicity. Frog-eye leaf lesions on CFP(+ )transgenic plants infected with Cercospora nicotianae conidia were smaller but were similar in number to those on non-transgenic plants. We conclude that transgenic expression of CFP may have relevance for a disease control strategy in Cercospora-plant pathosystems where cercosporin is implicated in pathogen virulence.}, number={20}, journal={BIOTECHNOLOGY LETTERS}, author={Upchurch, RG and Rose, MS and Eweida, M and Zuo, WN}, year={2005}, month={Oct}, pages={1543–1550} }
 @article{upchurch_rose_eweida_callahan_2002, title={Transgenic assessment of CFP-mediated cercosporin export and resistance in a cercosporin-sensitive fungus}, volume={41}, ISSN={["0172-8083"]}, DOI={10.1007/s00294-002-0280-4}, abstractNote={Cercosporin is a toxic polyketide produced by many phytopathogenic members of the fungal genus Cercospora. Cercospora species, themselves, exhibit the highest level of self-resistance to this almost universally toxic photosensitizer. Although the mechanism of cercosporin self-resistance is multi-faceted, partial resistance does appear to be provided by the encoded product of CFP ( cercosporin facilitator protein), a gene recently isolated from the pathogen of soybean, C. kikuchii. CFP has significant similarity to the major facilitator superfamily of integral membrane transport proteins. We expressed CFP in the cercosporin non-producing, cercosporin-sensitive fungus, Cochliobolus heterostrophus, in order to assess the transport activity of CFP and the contribution of CFP to cercosporin resistance in a fungal species free of endogenous toxin production. Expression of the CFP transgene in this fungus results in increased resistance to cercosporin due, apparently, to its export out of the fungus.}, number={1}, journal={CURRENT GENETICS}, author={Upchurch, RG and Rose, MS and Eweida, M and Callahan, TM}, year={2002}, month={Apr}, pages={25–30} }