Works (28)
Updated: August 2nd, 2023 21:16
2020 journal article
The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis
JOURNAL OF MOLECULAR BIOLOGY, 432(2), 343–357.
author keywords: Biofilms; Bacillus subtilis; Transcriptional regulation; NMR solution structure
MeSH headings : Amino Acid Sequence / genetics; Bacillus subtilis / genetics; Bacillus subtilis / ultrastructure; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / ultrastructure; Biofilms / growth & development; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / ultrastructure; Gene Expression Regulation, Bacterial / genetics; Molecular Docking Simulation; Mutation / genetics; Protein Binding / genetics; Protein Conformation
TL;DR:
This work presents the first full-length structural model of tetrameric SinR using a hybrid approach combining high-resolution solution NMR, chemical crosslinking, mass spectrometry, and molecular docking, and provides an evidence-based mechanism that confirms how disruption of the SinR tetramer by SinI regulates gene expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: February 27, 2020
2019 journal article
Translating antibody-binding peptides into peptoid ligands with improved affinity and stability
JOURNAL OF CHROMATOGRAPHY A, 1602, 284–299.
author keywords: Peptoid ligands; Molecular docking; Affinity chromatography; Antibodies; Protein A mimetic
MeSH headings : Adsorption; Alkalies / chemistry; Amino Acid Sequence; Animals; Antibodies / metabolism; Antibody Affinity; Binding Sites; CHO Cells; Cattle; Cricetinae; Cricetulus; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Ligands; Molecular Docking Simulation; Peptides / chemistry; Peptoids / chemistry; Protein Binding; Proteolysis; Temperature
TL;DR:
This work proposes a route for translation utilizing the IgG-binding peptide HWRGWV as reference sequence and forms a method guiding the design of peptoid variants of cognate peptide ligands, and help addressing the challenges that, despite the structural similarity, the peptide-to-peptoid translation presents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 16, 2019
2017 journal article
Design, selection, and development of cyclic peptide ligands for human erythropoietin
JOURNAL OF CHROMATOGRAPHY A, 1500, 105–120.
author keywords: Erythropoietin; Cyclic peptide ligands; Ligand screening; Affinity chromatography; Affinity maturation; In-silico docking
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Erythropoietin / chemistry; Humans; Kinetics; Ligands; Molecular Sequence Data; Peptides, Cyclic / chemistry; Peptides, Cyclic / isolation & purification; Protein Binding
TL;DR:
Mutagenesis studies performed on FSLLSH with natural and non-natural amino acid substitutions led to the identification of critical EPO-binding determinants, and the discovery of new peptide ligands that facilitate EPO recovery.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Structure of the Francisella response regulator QseB receiver domain, and characterization of QseB inhibition by antibiofilm 2-aminoimidazole-based compounds
MOLECULAR MICROBIOLOGY, 106(2), 223–235.
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Bacterial Proteins / ultrastructure; Biofilms / drug effects; Francisella tularensis / metabolism; Gene Expression Regulation, Bacterial / genetics; Imidazoles / metabolism; Imidazoles / pharmacology; Protein Binding; Virulence / drug effects; Virulence Factors / genetics; Virulence Factors / metabolism
TL;DR:
It is shown that QseB interacts with 2‐aminoimidazole based compounds that impede its function, and this information will assist in tailoring compounds to act as adjuvants that will enhance the effect of antibiotics.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Human Norovirus Aptamer Exhibits High Degree of Target Conformation-Dependent Binding Similar to That of Receptors and Discriminates Particle Functionality
MSPHERE, 1(6).
author keywords: aptamer; GII.4; histo-blood group antigen; infectivity; noroviruses
TL;DR:
A hitherto unexplored property of a different type of ligand, a nucleic acid aptamer, to mimic receptor binding behavior and assess capsid functionality for a selected strain of norovirus is reported, suggesting that other aptamers may show promise in the study of other fastidious microorganisms.
(via Semantic Scholar)
msphere.asm.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
10. Reduced Inequalities
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
NMR-based structural analysis of threonylcarbamoyl-AMP synthase and Its substrate interactions
Journal of Biological Chemistry, 290(33), 20032–20043.
Source: NC State University Libraries
Added: August 6, 2018
2014 journal article
A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA
JOURNAL OF MOLECULAR BIOLOGY, 426(9), 1911–1924.
author keywords: transition state regulator; AbbA; DNA mimic; molecular docking; NMR
MeSH headings : Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Docking Simulation; Protein Conformation; Protein Multimerization
TL;DR:
Interestingly, it is shown that AbbA has structural and surface characteristics that closely mimic the DNA phosphate backbone, enabling it to readily carry out its physiological function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Chemical shift assignments and secondary structure prediction of the phosphorelay protein VanU from Vibrio anguillarum
BIOMOLECULAR NMR ASSIGNMENTS, 8(1), 177–179.
author keywords: VanU; NMR; Vibrio anguillarum; Phosphorelay
MeSH headings : Bacterial Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular; Phosphotransferases / chemistry; Protein Structure, Secondary; Vibrio / metabolism
TL;DR:
The 1H, 13C, and 15N backbone and side chain resonance assignments and secondary structure prediction for VanU from V. anguillarum are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Structure and DNA-Binding Traits of the Transition State Regulator AbrB
STRUCTURE, 22(11), 1650–1656.
MeSH headings : Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Binding Sites; DNA, Bacterial / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Docking Simulation; Protein Multimerization; Protein Structure, Secondary; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
It is noted that AbrB possesses a strip of positive electrostatic potential encompassing its DNA-binding region and that its C-terminal domain aids in DNA binding.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
H-1, C-13, and N-15 resonance assignments and secondary structure prediction of the full-length transition state regulator AbrB from Bacillus anthracis
BIOMOLECULAR NMR ASSIGNMENTS, 6(1), 95–98.
author keywords: AbrB; NMR; Bacillus anthracis; Transition state regulator
MeSH headings : Bacillus anthracis; Bacterial Proteins / chemistry; DNA-Binding Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary; Transcription Factors / chemistry
TL;DR:
Backbone and sidechain resonance assignments and secondary structure prediction for the full-length AbrB protein from B. anthracis are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Identification of BfmR, a Response Regulator Involved in Biofilm Development, as a Target for a 2-Aminoimidazole-Based Antibiofilm Agent
BIOCHEMISTRY, 51(49), 9776–9778.
MeSH headings : Acinetobacter baumannii / drug effects; Acinetobacter baumannii / growth & development; Bacterial Proteins / chemistry; Bacterial Proteins / physiology; Biofilms / drug effects; Imidazoles / pharmacology; Microscopy, Confocal; Models, Molecular
TL;DR:
The 2AI compound studied here represents a unique small molecule scaffold that targets bacterial response regulators and is cell permeable, while pull-down assays identified BfmR, a response regulator that is the master controller of biofilm formation, as a target for this compound.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Structural insights into the calcium-dependent interaction between calbindin-D28K and caspase-3
FEBS LETTERS, 586(20), 3582–3589.
author keywords: Calbindin-D28K; Caspase-3; Isothermal titration calorimetry; Molecular docking
MeSH headings : Calbindins; Calcium / metabolism; Caspase 3 / chemistry; Caspase 3 / metabolism; Enzyme Activation; Hydrogen Bonding; Molecular Docking Simulation; Protein Binding; Protein Conformation; S100 Calcium Binding Protein G / chemistry; S100 Calcium Binding Protein G / metabolism
TL;DR:
Calbindin‐D28K and Caspase‐3 bind by isothermal titration calorimetry and this results in new insights into the mechanism by which these molecules bind to each other.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Binding site on human immunoglobulin G for the affinity ligand HWRGWV
Journal of Molecular Recognition, 23(3), 271–282.
Source: NC State University Libraries
Added: August 6, 2018
2010 journal article
Long range dynamic effects of point-mutations trap a response regulator in an active conformation
FEBS LETTERS, 584(19), 4203–4207.
author keywords: NMR; Conformational transition; Intra-protein communication; Response regulator; Spo0F
MeSH headings : Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Mutant Proteins / chemistry; Mutant Proteins / genetics; Mutant Proteins / metabolism; Nuclear Magnetic Resonance, Biomolecular; Point Mutation; Principal Component Analysis; Protein Conformation; Second Messenger Systems / genetics; Signal Transduction / genetics; Thermodynamics
TL;DR:
It is shown that point‐mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation, which results in functionally hyperactive Spo0f.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
NMR Solution Structure and DNA-binding Model of the DNA-binding Domain of Competence Protein A
JOURNAL OF MOLECULAR BIOLOGY, 398(2), 248–263.
author keywords: competence; ComA; NMR; two-component; response regulator
MeSH headings : Amino Acid Sequence; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; DNA / chemistry; DNA / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Helix-Turn-Helix Motifs; Models, Molecular; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Tertiary
TL;DR:
Using NMR spectroscopy, the high-resolution 3D solution structure of the C-terminal DNA-binding domain of ComA (ComAC) has been determined and a model of ComAC bound to the ComA recognition sequences on the srfA promoter has been developed, suggesting a basis for DNA binding specificity within the NarL family.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Structural Characterization of the Conformational Change in Calbindin-D-28k upon Calcium Binding Using Differential Surface Modification Analyzed by Mass Spectrometry
BIOCHEMISTRY, 48(36), 8603–8614.
MeSH headings : Amino Acid Sequence; Animals; Apoproteins / chemistry; Calbindin 1; Calbindins; Cysteine / chemistry; Disulfides / chemistry; EF Hand Motifs; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Rats; S100 Calcium Binding Protein G / chemistry; S100 Calcium Binding Protein G / metabolism; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
TL;DR:
Differential surface modification of lysine and histidine residues analyzed by mass spectrometry has been used in this study to identify, for the first time, the specific regions of calbindin-D28k undergoing conformational changes between the holo and apo states and indicate that there is predominantly no disulfide bond between these residues in the apoprotein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Co-Evolving Motions at Protein−Protein Interfaces of Two-Component Signaling Systems Identified by Covariance Analysis†
Biochemistry, 47(30), 7782–7784.
MeSH headings : Analysis of Variance; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Binding Sites; Magnetic Resonance Spectroscopy; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins / chemistry; Proteins / metabolism; Signal Transduction
TL;DR:
C covariance is shown to identify a cluster of co-evolving dynamic residues in two-component proteins and NMR dynamics and structural studies suggest that motions serve to precisely arrange the site of phosphoryl transfer within the complex.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2008 journal article
Insights into the Nature of DNA Binding of AbrB-like Transcription Factors
STRUCTURE, 16(11), 1702–1713.
MeSH headings : Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Binding Sites; DNA / chemistry; DNA / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Conformation; Thermodynamics; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT are presented and a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8 is generated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Targeting RNA with cysteine-constrained peptides
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 18(2), 565–567.
author keywords: RNA; cyclic peptides; HADDOCK
MeSH headings : Base Sequence; Cysteine / chemistry; Humans; Models, Molecular; Peptides, Cyclic / chemistry; RNA / chemistry
TL;DR:
This approach has successfully identified novel cyclic peptide constructs that can target bTAR RNA and RNA/peptide interactions were effectively modeled using the HADDOCK docking program.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Abh and AbrB control of Bacillus subtilis antimicrobial gene expression
JOURNAL OF BACTERIOLOGY, 189(21), 7720–7732.
MeSH headings : Bacillus subtilis / genetics; Bacillus subtilis / growth & development; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Binding Sites; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Kinetics; Molecular Sequence Data; Mutation; Plasmids; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR:
Abh is part of the complex interconnected regulatory system that controls gene expression during the transition from active growth to stationary phase and is subject to a degree of AbrB-affording negative regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Peptide binding proclivities of calcium loaded calbindin-D28k
FEBS LETTERS, 581(24), 4778–4782.
author keywords: calbindin-D28k; NMR; chemical shift; peptides; modeling
MeSH headings : Animals; Calbindin 1; Calbindins; Calcium / metabolism; Circular Dichroism; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Peptides / metabolism; Protein Binding; Protein Structure, Tertiary; Rats; S100 Calcium Binding Protein G / metabolism; Titrimetry
TL;DR:
It is shown that calbindin‐D28k undergoes significant conformational changes upon binding calcium, whereas only minor changes occur when binding target peptides in its Ca2+‐loaded state.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition
FEBS LETTERS, 581(7), 1425–1429.
author keywords: sporulation; kinases; Spo0F mutants
MeSH headings : Alanine / chemistry; Alanine / genetics; Amino Acid Sequence; Amino Acid Substitution; Bacillus subtilis / metabolism; Bacillus subtilis / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Histidine Kinase; Nuclear Magnetic Resonance, Biomolecular; Protein Conformation; Protein Interaction Mapping; Protein Kinases / chemistry; Protein Kinases / metabolism; Protein Structure, Secondary; Spores, Bacterial
TL;DR:
How buried residues and intra‐protein communication networks contribute to precise molecular recognition by ensuring that the correct surface is presented is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Tandem mass spectrometry acquisition approaches to enhance identification of protein-protein interactions using low-energy collision-induced dissociative chemical crosslinking reagents
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(21), 3395–3408.
MeSH headings : Amino Acid Sequence; Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Cross-Linking Reagents / chemistry; DNA-Binding Proteins / chemistry; Molecular Sequence Data; Peptide Mapping; Peptides / chemistry; Protein Conformation; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry / methods; Transcription Factors / chemistry
TL;DR:
The ability to segregate intrapeptide and interpeptide crosslinks using ITCID represents the first step towards high-throughput analysis of protein-protein cross links using the CID-CXL reagents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
NMR structure of AbhN and comparison with AbrBN - First insights into the DNA binding promiscuity and specificity of AbrB-like transition state regulator proteins
JOURNAL OF BIOLOGICAL CHEMISTRY, 281(30), 21399–21409.
MeSH headings : Amino Acid Sequence; Bacillus subtilis / enzymology; Bacterial Proteins / metabolism; Cloning, Molecular; DNA / chemistry; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Deoxyribonuclease I / chemistry; In Vitro Techniques; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Protein Binding; Sequence Homology, Amino Acid; Transcription Factors / metabolism
TL;DR:
The high resolution NMR solution structure of the N-terminal domain of the Bacillus subtilis transition state regulator Abh (AbhN) is presented, only the second such structure to date, and the results of accompanying in vitro DNA-binding studies serve to highlight binding differences between the two proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins
FEBS LETTERS, 579(25), 5669–5674.
author keywords: NMR solution structure; protein-DNA interactions; structural genomics; structural classification of proteins
MeSH headings : Amino Acid Sequence; Bacillus subtilis / metabolism; Bacterial Proteins; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / classification; Dimerization; Escherichia coli Proteins / chemistry; Molecular Sequence Data; Protein Structure, Tertiary; Transcription Factors / chemistry; Transcription Factors / classification
TL;DR:
The putative DNA‐binding site of AbrB is found on the same face as the DNA‐ binding site of MazE and appears similar, both in structure and sequence, to the exposed conserved region of MraZ, which strongly suggests that MRAZ also binds DNA and allows for a consensus model of DNA recognition by the members of this novel protein superfamily.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2004 journal article
Evaluation of the DNA Binding Tendencies of the Transition State Regulator AbrB†
Biochemistry, 43(51), 16106–16118.
MeSH headings : Bacillus subtilis / metabolism; Circular Dichroism; DNA / chemistry; DNA / metabolism; Kinetics; Protein Binding / physiology; Spectrometry, Mass, Electrospray Ionization; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
This comprehensive and corroborative spectroscopic study endorses the use of microESI-MS for rapidly ascertaining qualitative binding trends in noncovalent systems in a high-throughput manner.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2002 journal article
Macromolecular assembly of the transition state regulator AbrB in its unbound and complexed states probed by microelectrospray ionization mass spectrometry
ANALYTICAL BIOCHEMISTRY, 306(2), 222–227.
author keywords: AbrB; transition-state regulator; macromolecular assembly; mass spectrometry
MeSH headings : Bacillus subtilis; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Chromatography, Gel; DNA / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Escherichia coli; Macromolecular Substances; Protein Binding; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Spectrometry, Mass, Electrospray Ionization; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
This study represents the first detailed characterization of the stoichiometry of a transition-state regulator binding to one of its target promoters and demonstrates a capacity for DNA binding, underscoring the role of the N-terminal domain in both DNA recognition and dimerization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Stoichiometries of protein - Protein/DNA binding and conformational changes for the transition-state regulator AbrB measured by pseudo cell-size exclusion chromatography-mass spectrometry
BIOCHEMISTRY, 41(25), 7859–7865.
MeSH headings : Bacillus subtilis / chemistry; Bacterial Proteins / chemistry; Chromatography, Gel / instrumentation; Chromatography, Gel / methods; DNA / chemistry; DNA-Binding Proteins / chemistry; Electron Transport; Electron-Transferring Flavoproteins; Flavoproteins / chemistry; Geobacillus stearothermophilus / chemistry; Humans; Mass Spectrometry / instrumentation; Mass Spectrometry / methods; Protein Binding; Protein Conformation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Transcription Factors / chemistry
TL;DR:
On-line pseudo cell-size exclusion chromatography-mass spectrometry (PsC-SEC-MS) enables the efficient determination of gross conformational changes upon complexation and shows that when the complex binds to sinIR, the hydrodynamic volume of the complex is notably reduced compared to that of the apoprotein, indicating a protein conformational change.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
10. Reduced Inequalities
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
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