@article{tremblay_vaewhongs_turner_sit_lommel_2005, title={Cell wall localization of Red clover necrotic mosaic virus movement protein is required for cell-to-cell movement}, volume={333}, ISSN={["0042-6822"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-13644249879&partnerID=MN8TOARS}, DOI={10.1016/j.virol.2004.12.019}, abstractNote={The Red clover necrotic mosaic virus movement protein (MP) is essential for cell-to-cell movement. Eight previously characterized alanine-scanning mutants of the MP were fused to the green fluorescent protein (GFP) and expressed from viral infectious transcripts. Inoculated plants were assayed for movement and intracellular accumulation of MP by confocal laser-scanning microscopy. A strict correlation was observed between the targeting to the cell wall (presumably the plasmodesmata) and cell-to-cell movement. Complementation of dysfunctional MP mutants with either wild-type MP or other null mutants in some cases rescued intracellular targeting and movement. The data suggest the presence of distinct domains in the MP for virus movement (near residues 27-31), complementarity (near residues 122 and 128), and intracellular localization (near residue 161). These data support a model of MP interacting cooperatively with itself to bind viral RNA, localize to and modify plasmodesmata and effect virus movement.}, number={1}, journal={VIROLOGY}, publisher={Elsevier BV}, author={Tremblay, D and Vaewhongs, AA and Turner, KA and Sit, TL and Lommel, SA}, year={2005}, month={Mar}, pages={10–21} }
 @article{fellers_tremblay_handest_lommel_2002, title={The Potato virus Y (MNR)-N-S Nlb-replicase is the elicitor of a veinal necrosis-hypersensitive response in root knot nematode resistant tobacco}, volume={3}, ISSN={["1464-6722"]}, DOI={10.1046/j.1364-3703.2002.00106.x}, abstractNote={SummaryA root knot nematode resistance gene in Nicotiana tabacum, Rk, providing resistance to the nematode parasite Meloidogyne incognita is tightly linked to, or is a pleiotropic gene with a veinal necrosis systemic hypersensitive response to infection by Potato virus Y (PVY) MsNr. The single PVY MsNr open reading frame was sequenced and found to have 89% protein identity to PVY N. Individual PVY MsNr polypeptides were deduced and the corresponding cDNA were cloned into a Potato virus X (PVX) based expression vector and used as templates for in vitro transcriptions. Infected plant sap, from N. benthamiana inoculated with infectious RNA, was used to inoculate both root knot nematode (RKN) resistant and susceptible tobacco lines. Lines were then evaluated for the induction of the hypersensitive response. The PVY MsNr NIb‐replicase protein was found to induce a hypersensitive response 10 days post inoculation in nematode resistant tobacco. None of the other PVX/PVY MsNr constructs induced a hypersensitive response. The NIb‐replicase of PVY N, which shares 93% identity to PVY MsNr, did not induce a hypersensitive response when expressed from the PVX vector. This confirmed that the PVY MsNr NIb‐replicase is the elicitor of PVY MsNr veinal necrosis on RKN plants and thus the first report of a Potyvirus replicase functioning as an avirulence factor.}, number={3}, journal={MOLECULAR PLANT PATHOLOGY}, author={Fellers, JP and Tremblay, D and Handest, MF and Lommel, SA}, year={2002}, month={May}, pages={145–152} }