@article{dil_qureshi_2003, title={Interleukin-1 beta does not contribute to genetic strain-based differences in iNOS expression and activity in chicken macrophages}, volume={27}, ISSN={["0145-305X"]}, DOI={10.1016/S0145-305X(02)00075-7}, abstractNote={The expression of IL-1beta and inducible nitric oxide synthase (iNOS) from iNOS hypo (GB2, B(6)B(6)) and hyper (K-strain, B(15)B(15)) responder chickens was examined. Compared to GB2, macrophages from K-strain expressed higher iNOS mRNA as quantitated by reverse transcriptase polymerase (RT-PCR) chain reaction after stimulation with 1 microgram/ml of Escherichia coli (E. coli) lipopolysaccharide (LPS). On the contrary, IL-1beta mRNA expression was comparable between K and GB2 macrophages at 3h post-LPS stimulation but persisted up to 9h only in GB2 macrophages. The LPS-inducible interleukin-1 (IL-1) surface receptor expression, measured by flow cytometry, was higher in GB2 than on K-strain macrophages. Blocking of IL-1 receptor by the anti-IL-1 receptor antibody reduced the LPS-mediated iNOS expression by 50% as quantified by competitive RT-PCR. Furthermore, iNOS activity (nitrite) was also reduced to 50%. However, this magnitude of inhibition was similar in both K and GB2 macrophages. While these observations suggest that IL-1beta is involved in mediating LPS-induced iNOS expression and activity, the differential response of GB1 and K-strain macrophages in terms of LPS-induced iNOS expression and activity is unlikely to be modulated by IL-1beta.}, number={2}, journal={DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY}, author={Dil, N and Qureshi, MA}, year={2003}, month={Feb}, pages={137–146} }
 @article{dil_qureshi_2002, title={Involvement of lipopolysaccharide related receptors and nuclear factor kappa B in differential expression of inducible nitric oxide synthase in chicken macrophages from different genetic backgrounds}, volume={88}, ISSN={["1873-2534"]}, DOI={10.1016/S0165-2427(02)00153-8}, abstractNote={Macrophages from Cornell K-strain chickens (B(15)B(15)) are hyper and from GB2 chickens (B(6)B(6)) are hypo-responders to LPS-mediated inducible NOS (iNOS) expression and activity. The molecular mechanism(s) responsible for this differential expression is not yet fully understood. We have previously reported that macrophages from K (iNOS hyper-responder) and GB2 (iNOS hypo-responder) chickens differ in constitutive expression of TLR4 but not in CD14 molecules. The objectives of the current study was to determine if the iNOS differences between K and GB2 macrophages are possibly due to differential expression of LPS-induced TLR4, CD14 and/or nuclear factor kappa B (NF kappa B). The results showed that Sephadex-elicited, adherence purified K macrophages expressed more inducible TLR4 and CD14 receptors (P<0.05) at 6 and 12h post-LPS stimulation than GB2 macrophages as measured by flow cytometry. In addition, pre-incubation of macrophages from a transformed chicken macrophage cell line, MQ-NCSU, with 50 microg/ml anti-CD14 and anti-TLR4 antibodies significantly reduced where as pre-incubation with 100 microg/ml completely blocked LPS-mediated iNOS activity as measured by nitrite levels. Furthermore, the amount of nuclear bound NF kappa B was found to be significantly greater in K than in GB2 macrophages at 3 min post-LPS stimulation. This nuclear localization of NF kappa B as well as iNOS activity was completely inhibited by pretreatment of macrophages with 50 micro M MG132, a proteosome inhibitor, both in K and GB2 macrophages. Taken together, these findings suggest that a differential and perhaps more stronger LPS-mediated signaling via CD14, TLR4 and NF kappa B is responsible for the heightened iNOS gene induction in K-strain (hyper-responder) macrophages than in GB2 (hypo-responder) chickens.}, number={3-4}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={Dil, N and Qureshi, MA}, year={2002}, month={Sep}, pages={149–161} }