@article{joobeur_gusmini_zhang_levi_xu_wehner_oliver_dean_2006, title={Construction of a watermelon BAC library and identification of SSRs anchored to melon or Arabidopsis genomes}, volume={112}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-006-0258-6}, abstractNote={A bacterial artificial chromosome (BAC) library was constructed for watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus) with an average insert-size of 106 kb, providing 21 haploid genome equivalents. The library was used to identify BAC clones that are anchored to probes evenly distributed on the genomes of melon or Arabidopsis. Twenty eight probes (representing 66% of the tested probes) from melon and 30 probes (65%) from Arabidopsis identified positive BAC clones. Two methods were implemented to identify SSRs from the positively hybridizing BAC clones. First, analysis of BAC end sequences revealed 37 SSRs. For the second method, pooled DNA of BACs identified by the melon probes was used to develop a shotgun library. The library was then screened with synthetic SSR oligonucleotides by hybridization. Sequence analysis of positively hybridizing shotgun clones revealed 142 different SSRs. Thirty eight SSRs were characterized using three watermelon cultivars, five plant introduction (PI) accessions of C. lanatus var lanatus and four PIs of C. lanatus var citroides. Of these, 36 (95%) were found to be polymorphic with up to six alleles per marker. Polymorphism information content values for polymorphic markers varied between 0.22 and 0.79 with an average of 0.53. The methods described herein will be valuable for the construction of a watermelon linkage map with SSRs evenly distributed on its genome that is anchored to the genomes of melon and Arabidopsis.}, number={8}, journal={THEORETICAL AND APPLIED GENETICS}, author={Joobeur, T. and Gusmini, G. and Zhang, X. and Levi, A. and Xu, Y. and Wehner, T. C. and Oliver, M. and Dean, R. A.}, year={2006}, month={May}, pages={1553–1562} } @article{gusmini_wehner_joobeur_dean_levi_2004, title={Protocol for DNA extraction from watermelon leaves for SSR marker studies}, ISBN={1064-5594}, number={27}, journal={Report (Cucurbit Genetics Cooperative)}, author={Gusmini, G. and Wehner, T. C. and Joobeur, T. and Dean, R. A. and Levi, A.}, year={2004}, pages={25} } @article{joobeur_king_nolin_thomas_dean_2004, title={The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features}, volume={39}, ISSN={["1365-313X"]}, DOI={10.1111/j.1365-313X.2004.02134.x}, abstractNote={Summary}, number={3}, journal={PLANT JOURNAL}, author={Joobeur, T and King, JJ and Nolin, SJ and Thomas, CE and Dean, RA}, year={2004}, month={Aug}, pages={283–297} } @article{levi_thomas_joobeur_zhang_davis_2002, title={A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides x C-lanatus var. lanatus) x Citrullus colocynthis}, volume={105}, ISSN={["1432-2242"]}, DOI={10.1007/S00122-001-0860-6}, abstractNote={A genetic linkage map was constructed for watermelon using a testcross population [Plant Accession Griffin 14113 ( Citrullus lanatus var. citroides) x New Hampshire Midget (NHM; C. lanatus var. lanatus)] x U.S. Plant Introduction (PI) 386015 ( Citrullus colocynthis). The map contains 141 randomly amplified polymorphic DNA (RAPD) markers produced by 78 primers, 27 inter-simple sequence repeat (ISSR) markers produced by 17 primers, and a sequence-characterized amplified region (SCAR) marker that was previously reported as linked (1.6 cM) to race-1 Fusarium wilt [incited by Fusarium oxysporum Schlechtend.:Fr. f. sp. niveum (E.F.Sm.) W.C. Synder & H.N. Hans] resistance in watermelon. The map consists of 25 linkage groups. Among them are a large linkage group that contains 22 markers covering a mapping distance of 225.6 cM and six large groups each with 10-20 markers covering a mapping distance of 68.8 to 110.8 cM. There are five additional linkage groups consisting of 3-7 markers per group, each covering a mapping distance of 36.5 to 57.2 cM. The 13 remaining linkage groups are small, each consisting of 2-11 markers covering a mapping distance of 3.5-29.9 cM. The entire map covers a total distance of 1,166.2 cM with an average distance of 8.1 cM between two markers. This map is useful for the further development of markers linked to disease resistance and watermelon fruit qualities.}, number={4}, journal={THEORETICAL AND APPLIED GENETICS}, author={Levi, A and Thomas, CE and Joobeur, T and Zhang, X and Davis, A}, year={2002}, month={Sep}, pages={555–563} }