Works (1)
Updated: July 5th, 2023 15:59
2002 journal article
Overexpression of peptidases in Lactococcus and evaluation of their release from leaky cells
JOURNAL OF DAIRY SCIENCE, 85(10), 2438–2450.
author keywords: leaky Lactococcus; peptidase
MeSH headings : Aminopeptidases / genetics; Aminopeptidases / metabolism; Bacterial Proteins; Cloning, Molecular; Coumarins / metabolism; DNA Restriction Enzymes; Escherichia coli / genetics; Gene Expression; Genetic Vectors; Glutamyl Aminopeptidase; Lactococcus / enzymology; Lactococcus / genetics; Lactococcus lactis / enzymology; Lactococcus lactis / genetics; Metalloendopeptidases / genetics; Metalloendopeptidases / metabolism; Oligopeptides / metabolism; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Plasmids / genetics; Promoter Regions, Genetic; Substrate Specificity; Transformation, Bacterial
TL;DR:
Results showed different levels of enzymatic overexpression associated with the cellular fraction and negligible amounts of activity present within the supernatant fraction of leaky lactococcal peptidases investigated in this study.
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