@article{cullison_jaykus_2002, title={Magnetized carbonyl iron and insoluble zirconium hydroxide mixture facilitates bacterial concentration and separation from nonfat dry milk}, volume={65}, ISSN={["0362-028X"]}, DOI={10.4315/0362-028X-65.11.1806}, abstractNote={A mixture of magnetized carbonyl iron and insoluble zirconium hydroxide was investigated for its ability to concentrate various foodborne pathogens from 25-ml samples of reconstituted nonfat dry milk. Each sample was artificially contaminated with 10(3) to 10(6) CFU/25 ml of representative foodborne pathogens (Salmonella enterica serovar Enteritidis, Listeria monocytogenes, and Bacillus cereus spores) and processed for bacterial concentration with high-speed centrifugation for the primary concentration followed by a secondary concentration step involving the carbonyl iron-zirconium hydroxide mixture. Bacterial recoveries, as evaluated on the basis of loss to discarded supernatants, exceeded 75% for all organisms at all inoculum levels and were usually >90%. Recovery was confirmed by direct plating of the immobilized pellet, for which the valueswere similar albeit more varied. Additional experiments confirmed that the magnetized carbonyl iron-insoluble zirconium hydroxide mixture was relatively nontoxic to both Salmonella Enteritidis and L monocytogenes Overall, the entire concentration scheme resulted in a 25-fold reduction in sample volume with the recovery of viable bacterial cells. This novel compound shows promise for facilitating inexpensive, rapid, and effective bacterial concentration in food systems.}, number={11}, journal={JOURNAL OF FOOD PROTECTION}, author={Cullison, MA and Jaykus, LA}, year={2002}, month={Nov}, pages={1806–1810} }