Feng Zhao

Works (1)

Updated: July 5th, 2023 15:59

2002 article

Purification and Characterization of the Fusion Protein Trypsin-Streptavidin Expressed in Escherichia coli

Zhao, F., Clare, D. A., Catignani, G. L., & Swaisgood, H. E. (2002, August 1). Journal of Protein Chemistry.

By: F. Zhao n, D. Clare n, G. Catignani n & H. Swaisgood n

author keywords: affinity column; fusion protein; protein expression; protein purification; trypsin; streptavidin
MeSH headings : Blotting, Western; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Streptavidin / chemistry; Trypsin / chemistry
topics (OpenAlex): Biotin and Related Studies; Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research
TL;DR: The molecular size of the soluble purified fusion protein was determined by size-exclusion chromatography using Superose 12 FPLC, indicating that the soluble protein exists as a monomer; thus, the presence of the trypsin domain must prevent the streptavidin domain from tetramer formation. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2026) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.