@misc{orndorff_hamrick_smoak_havell_2006, title={Host and bacterial factors in listeriosis pathogenesis}, volume={114}, ISSN={["1873-2542"]}, DOI={10.1016/j.vetmic.2005.12.003}, abstractNote={Members of the Genus Listeria are ubiquitous environmental saprophytic microorganisms. If ingested they can cause a severe disseminated disease (listeriosis) that has a high mortality rate, the highest of any food-borne pathogen, even with antibiotic therapy. Central to the high mortality rate is the hallmark characteristic of the microorganism to grow intracellularly. The presence of listeriae in food processing plants has resulted in many outbreaks of human disease and large scale recalls of processed foods. Despite the ubiquity of the microorganism, the actual disease rate (those animals showing disease signs over those exposed) is quite low and disease is almost always associated with an underlying predisposition (pregnancy being the most common in otherwise normal individuals). There are many features of the pathogenesis of listeriosis that have remained mysterious despite the extensive use of the microorganism in the study of cell-mediated immunity and intracellular growth. Informational advances such as the sequence of the mouse and listerial genomes, and technical advances such as the discovery of listeria-susceptible mouse strains, may renew interest in the study of the natural pathogenesis of the disease. This may be further facilitated by studies that employ the natural inoculation route and mimic common predisposing conditions witnessed in victims of natural outbreaks.}, number={1-2}, journal={VETERINARY MICROBIOLOGY}, author={Orndorff, PE and Hamrick, TS and Smoak, IW and Havell, EA}, year={2006}, month={Apr}, pages={1–15} }
 @article{cesta_baty_keene_smoak_malarkey_2005, title={Pathology of end-stage remodeling in a family of cats with hypertrophic cardiomyopathy}, volume={42}, ISSN={["1544-2217"]}, DOI={10.1354/vp.42-4-458}, abstractNote={ End-stage hypertrophic cardiomyopathy (ES-HCM), affecting 5-10% of human hypertrophic cardiomyopathy (HCM) patients, is characterized by relative thinning of the ventricular walls and septum with dilation of the ventricular lumen, decreased fractional shortening, and progression to heart failure. C. J. Baty and others recently documented similar progressive changes to ES-HCM in a family of four cats through serial echocardiograms. At the time of heart failure, these cats exhibited changes similar to those exhibited by human ES-HCM patients. Our objectives were to describe the pathologic alterations associated with ES-HCM and investigate the pathogenesis in three of the four cats. Grossly, there was left atrial dilation with relative thinning of the interventricular septum (IVS) and left ventricular free wall (LVFW). The left atrium contained large thrombi in two of the three cats, and all three cats died following thromboembolization of the aortic bifurcation. Histologically, all three cats had subendocardial and myocardial fibrosis, predominantly of the IVS and LVFW, and one cat had acute, multifocal, myocardial infarcts with mononuclear inflammatory cell infiltrates. The pathogenesis of ES-HCM is uncertain, but theories implicate occlusion of the coronary blood flow by thickening of the coronary vessels, coronary vascular thromboembolism or coronary vessel spasm, apoptosis of myocytes, and myocardial hypertrophy beyond the ability of the vasculature to supply blood. Apoptosis assays did not reveal any apoptotic myocytes. Considering the hypercoagulative state of these cats, coronary vascular thromboembolism could be a major contributing factor. We cannot exclude apoptosis or coronary vessel spasm on the basis of the data presented. }, number={4}, journal={VETERINARY PATHOLOGY}, author={Cesta, MF and Baty, CJ and Keene, BW and Smoak, IW and Malarkey, DE}, year={2005}, month={Jul}, pages={458–467} }
 @article{smoak_byrd_abu-issa_goddeeris_anderson_morris_yamamura_klingensmith_meyers_2005, title={Sonic hedgehog is required for cardiac outflow tract and neural crest cell development}, volume={283}, ISSN={["1095-564X"]}, DOI={10.1016/j.ydbio.2005.04.029}, abstractNote={The Hedgehog signaling pathway is critical for a significant number of developmental patterning events. In this study, we focus on the defects in pharyngeal arch and cardiovascular patterning present in Sonic hedgehog (Shh) null mouse embryos. Our data indicate that, in the absence of Shh, there is general failure of the pharyngeal arch development leading to cardiac and craniofacial defects. The cardiac phenotype results from arch artery and outflow tract patterning defects, as well as abnormal development of migratory neural crest cells (NCCs). The constellation of cardiovascular defects resembles a severe form of the human birth defect syndrome tetralogy of Fallot with complete pulmonary artery atresia. Previous studies have demonstrated a role for Shh in NCC survival and proliferation at later stages of development. Our data suggest that SHH signaling does not act directly on NCCs as a survival factor, but rather acts to restrict the domains that NCCs can populate during early stages (e8.5-10.5) of cardiovascular and craniofacial development.}, number={2}, journal={DEVELOPMENTAL BIOLOGY}, author={Smoak, IW and Byrd, NA and Abu-Issa, R and Goddeeris, MM and Anderson, R and Morris, J and Yamamura, K and Klingensmith, J and Meyers, EN}, year={2005}, month={Jul}, pages={357–372} }
 @article{smoak_2004, title={Hyperglycemia-induced TGF beta and fibronectin expression in embryonic mouse heart}, volume={231}, ISSN={["1058-8388"]}, DOI={10.1002/dvdy.20123}, abstractNote={Abstract}, number={1}, journal={DEVELOPMENTAL DYNAMICS}, author={Smoak, IW}, year={2004}, month={Sep}, pages={179–189} }
 @article{ghatnekar_barnes_dow_smoak_2004, title={Hypoglycemia-induced changes in cell death and cell proliferation in the organogenesis-stage embryonic mouse heart (Retracted article. See vol 76, pg 278, 2006)}, volume={70}, ISSN={["1542-0760"]}, DOI={10.1002/bdra.20000}, abstractNote={Abstract}, number={3}, journal={BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY}, author={Ghatnekar, GS and Barnes, JA and Dow, JL and Smoak, IW}, year={2004}, month={Mar}, pages={121–131} }
 @article{joyner_smoak_2004, title={In vivo hyperglycemia and its effect on Glut-1 expression in the embryonic heart}, volume={70}, ISSN={["1542-0760"]}, DOI={10.1002/bdra.20046}, abstractNote={Abstract}, number={7}, journal={BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY}, author={Joyner, NT and Smoak, IW}, year={2004}, month={Jul}, pages={438–448} }
 @article{hamrick_horton_spears_havell_smoak_orndorff_2003, title={Influence of pregnancy on the pathogenesis of listeriosis in mice inoculated intragastrically}, volume={71}, ISSN={["1098-5522"]}, DOI={10.1128/IAI.71.9.5202-5209.2003}, abstractNote={ABSTRACT}, number={9}, journal={INFECTION AND IMMUNITY}, author={Hamrick, TS and Horton, JR and Spears, PA and Havell, EA and Smoak, IW and Orndorff, PE}, year={2003}, month={Sep}, pages={5202–5209} }
 @article{ghatnekar_gracz_smoak_2002, title={C-13-NMR study of hypoglycemia-induced glycolytic changes in embryonic mouse heart}, volume={66}, ISSN={["0040-3709"]}, DOI={10.1002/tera.10103}, abstractNote={Abstract}, number={5}, journal={TERATOLOGY}, author={Ghatnekar, GS and Gracz, HS and Smoak, IW}, year={2002}, month={Nov}, pages={267–272} }
 @article{el-bayomy_smoak_branch_2002, title={Embryotoxicity of the pesticide mirex in vitro}, volume={22}, ISSN={["0270-3211"]}, DOI={10.1002/tcm.10016}, abstractNote={Abstract}, number={4}, journal={TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS}, author={El-Bayomy, AA and Smoak, IW and Branch, S}, year={2002}, pages={239–249} }
 @article{abu-issa_smyth_smoak_yamamura_meyers_2002, title={Fgf8 is required for pharyngeal arch and cardiovascular development in the mouse}, volume={129}, number={19}, journal={Development (Cambridge, England)}, author={Abu-Issa, R. and Smyth, G. and Smoak, I. and Yamamura, K. and Meyers, E. N.}, year={2002}, pages={4613–4625} }
 @misc{smoak_2002, title={Hypoglycemia and embryonic heart development}, volume={7}, ISSN={["1093-4715"]}, DOI={10.2741/smoak}, abstractNote={Abnormal embryonic development is a complication of the diabetic pregnancy, and heart defects are among the most common and detrimental congenital malformations of the diabetic embryopathy. Hypoglycemia is a common side effect of diabetes therapy and is a potential teratogen. An association between hypoglycemia and congenital defects has been difficult to demonstrate in humans, but in vivo and in vitro animal studies have illustrated the importance of glucose as a substrate for normal development. Hypoglycemia alters embryonic heart morphology, producing abnormal looping and chamber expansion, decreased myocardial thickness, disorganized layers, and decreased overall size. Hypoglycemia decreases embryonic heart rate and vascularity, and it alters embryonic heart metabolism by increasing glucose uptake and glycolysis. Hypoglycemia also affects protein expression in the embryonic heart, increasing the expression of glucose regulated proteins, hexokinase, and glucose transport protein. Thus, hypoglycemia interferes with normal cardiogenesis and alters morphology, function, metabolism, and expression of certain proteins in the developing heart. It is likely that these factors contribute to heart defects observed in the diabetic embryopathy, but the definitive link has yet to be made. Future studies are expected to further elucidate mechanisms mediating hypoglycemia-induced cardiac dysmorphogenesis.}, journal={FRONTIERS IN BIOSCIENCE-LANDMARK}, author={Smoak, IW}, year={2002}, month={Jan}, pages={D307–D318} }
 @article{smoak_2002, title={Tolbutamide alters glucose transport and metabolism in the embryonic mouse heart}, volume={65}, ISSN={["0040-3709"]}, DOI={10.1002/tera.1094}, abstractNote={Abstract}, number={1}, journal={TERATOLOGY}, author={Smoak, IW}, year={2002}, month={Jan}, pages={19–25} }
 @article{barnes_smoak_2000, title={Glucose-regulated protein 78 (GRP78) is elevated in embryonic mouse heart and induced following hypoglycemic stress}, volume={202}, ISSN={["0340-2061"]}, DOI={10.1007/s004290000090}, abstractNote={This study investigates the distribution and heart levels of glucose regulated protein (GRP) 78 during normal development and in response to hypoglycemia in the mouse. Results demonstrate that GRP78 is strongly expressed with in the heart, neural tube, gut endoderm, somites, and surface ectoderm of mouse embryos during early organogenesis, and GRP78 staining remains prominent in the heart from gestational days 9.5 through 13.5. Cardiac myocytes are the primary site of GRP78 expression within the heart. GRP78 levels are highest in the heart during early organogenesis and levels decrease significantly by the fetal period. GRP78 expression is increased after 24 h of hypoglycemia in the early organogenesis-stage heart. Considering the tissue specific pattern of GRP expression and changes during development of the heart, GRPs may play significant roles in the normal differentiation and development of cardiac tissue. GRP induction may also be involved in hypoglycemia-induced cardiac dysmorphogenesis.}, number={1}, journal={ANATOMY AND EMBRYOLOGY}, author={Barnes, JA and Smoak, IW}, year={2000}, month={Jul}, pages={67–74} }
 @article{smoak_branch_2000, title={Glut-1 expression and its response to hypoglycemia in the embryonic mouse heart}, volume={201}, ISSN={["0340-2061"]}, DOI={10.1007/s004290050321}, abstractNote={The embryonic heart depends on glucose during early organogenesis. Glut-1 functions in constitutive glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. This study focuses on Glut-1 expression in the heart during normal organogenesis using immunohistochemistry for Glut-1 distribution, Western analysis for Glut-1 protein levels, and reverse transcriptase polymerase chain reaction for Glut-1 mRNA levels. The role of Glut in glucose uptake response to hypoglycemia in the embryonic heart is evaluated using the Glut inhibitor cytochalasin B. Cardiac Glut-1 expression is also evaluated after in vitro hypoglycemic exposure. Glut-1 levels are highest on gestational days 9-10, intermediate on gestational day 10.5, and lowest on gestational days 11.5-13.5 in the normal embryonic heart. Cardiac Glut-1 mRNA levels similarly decline between gestational days 9.5 and gd 13.5. Cytochalasin B produces a dose-dependent decrease in glucose uptake in hearts exposed to hypoglycemia for 30 min or 6 h, implicating Glut in this response. Glut-1 protein expression is unchanged after 2 or 6 h but increased after 12 and 24 h of hypoglycemia in the gestational day 9.5 heart. Thus, Glut-1 expression is prominent in the embryonic heart and is correlated with changes in cardiac glucose requirements during normal organogenesis. Glut activity increases in response to acute hypoglycemia and the expression of Glut-1 increases in response to prolonged hypoglycemia. These results support the importance of Glut-1 during normal cardiogenesis and in response to hypoglycemia in the embryonic heart.}, number={5}, journal={ANATOMY AND EMBRYOLOGY}, author={Smoak, IW and Branch, S}, year={2000}, month={May}, pages={327–333} }
 @book{smoak_2000, title={Veterinary embryology and teratology}, ISBN={0966683250}, publisher={Weybridge, VT: Cherry Tree Books}, author={Smoak, I. W.}, year={2000} }
 @article{smoak_1999, title={Cromakalim: Embryonic effects and reduction of tolbutamide-induced dysmorphogenesis in vitro}, volume={60}, ISSN={["0040-3709"]}, DOI={10.1002/(SICI)1096-9926(199911)60:5<260::AID-TERA6>3.0.CO;2-P}, abstractNote={Cromakalim is a K(+) channel opener that causes smooth muscle relaxation by activating ATP-sensitive K(+) (K(ATP)) channels and producing membrane hyperpolarization. Cromakalim counteracts sulfonylurea-induced K(ATP) channel inhibition in adult cells, but little is known regarding its embryonic effects, alone or in combination with sulfonylureas. K(ATP) channels have been demonstrated in the embryo, but their role in normal and abnormal development is unknown. Early-somite mouse embryos were exposed for 24 hr in vitro to cromakalim at concentrations of 0 (Cntl), 1, 10, 100, 200, or 500 microM in 0.125% DMSO. Embryos were also exposed for 24 hr in vitro to a dysmorphogenic tolbutamide concentration (110 microg/ml) combined with a subdysmorphogenic concentration of cromakalim (1 microM). Embryos were evaluated for somite number, heart rate, malformations, and embryonic and yolk sac protein content. Embryos exposed to 1 microM cromakalim were similar to controls. Cromakalim exposure increased malformation rates at concentrations >/=200 microM, decreased heart rates at >/=10 microM, and decreased somite and protein values at 500 microM. Defects involved cranial neural tube, optic vesicle, heart, and somites. A malformation rate of 59% in embryos exposed to 110 microg/ml tolbutamide was reduced to 13% by adding 1 microM cromakalim to the culture medium. Heart rate, somite number, and protein values were also improved by combined exposure to cromakalim and tolbutamide compared with exposure to tolbutamide alone. These results support previous findings with diazoxide (K(+) channel opener) and chlorpropamide (sulfonylurea) and further suggest a potential role for K(ATP) channel effects in sulfonylurea-induced dysmorphogenesis.}, number={5}, journal={TERATOLOGY}, author={Smoak, IW}, year={1999}, month={Nov}, pages={260–264} }
 @article{barnes_smoak_branch_1999, title={Expression of glucose-regulated proteins (GRP78 and GRP94) in hearts and fore-limb buds of mouse embryos exposed to hypoglycemia in vitro}, volume={4}, ISSN={["1355-8145"]}, DOI={10.1379/1466-1268(1999)004<0250:EOGRPG>2.3.CO;2}, abstractNote={Hypoglycemia, the classic inducer of glucose-related protein (GRP) synthesis, is dysmorphogenic in rodent embryos and detrimentally affects the heart. This study compares GRP induction in a target vs non-target tissue by evaluating GRP expression in hearts and fore-limb buds of mouse embryos following exposure to hypoglycemia in vitro. Gestational day 9.5 embryos were exposed to 2, 6, and 24 h of either mild (80 mg/dl glucose) or severe (40 mg/dl glucose) hypoglycemia using the method of whole-embryo culture. GRP78 increased in a dose- and time-dependent fashion in embryonic hearts exposed to either 40 mg/dl or 80 mg/dl glucose, whereas GRP94 levels increased in hearts only after 24 h of hypoglycemia. In contrast to the heart, GRP induction in fore-limb buds occurred only with GRP78 following the most severe level and duration of hypoglycemia. RT-PCR analysis demonstrated an elevation in GRP78 and GRP94 message levels in embryonic hearts following severe hypoglycemia. However, mRNA levels did not increase in response to mild hypoglycemia. Overall, these data demonstrate the preferential induction of GRPs in the heart as compared to fore-limb buds in mouse embryos exposed to hypoglycemia. Increases in GRP protein levels may be a more reliable biomarker of stress than message levels. However, both tissues and methods should be examined for enhanced biomarker sensitivity.}, number={4}, journal={CELL STRESS & CHAPERONES}, author={Barnes, JA and Smoak, IW and Branch, S}, year={1999}, month={Dec}, pages={250–258} }
 @article{smoak_1999, title={Glucose metabolism in mouse embryos exposed to metformin in vitro}, volume={13}, ISSN={["0887-2333"]}, DOI={10.1016/S0887-2333(98)00061-7}, abstractNote={Metformin is an oral hypoglycaemic agent used to treat patients with non-insulin-dependent diabetes mellitus, but its effect on embryonic tissues has not been well studied. Early-somite mouse embryos were exposed in whole embryo culture to metformin (0-2000mug/ml) and assayed for glucose uptake and glycolysis at 6, 12 and 24 hours. Embryos exposed to metformin for 6 hours were also evaluated for glucose uptake in the presence of 0 or 100 mum cytochalasin B. Glucose uptake was increased in embryos exposed to metformin at 2000mug/ml for 6 hours and 1000mug/ml or more for 12 hours. Glycolysis was increased in embryos exposed to metformin at 2000mug/ml for 6 or 24 hours and 1000mug/ml or more for 12 hours, producing lactate concentrations up to six times higher than controls. Glut-1 was increased in embryos exposed to 1000mug/ml or more for 6 hours, and metformin-stimulated glucose uptake was significantly decreased by cytochalasin B. Thus, glucose uptake and glycolytic metabolism are increased in early-somite mouse embryos in response to high concentrations of metformin in vitro, and the mechanism of increased glucose uptake appears to involve a cytochalasin B-sensitive glucose transporter.}, number={1}, journal={TOXICOLOGY IN VITRO}, author={Smoak, IW}, year={1999}, month={Feb}, pages={27–33} }
 @article{fritz_smoak_branch_1999, title={Hexokinase I expression and activity in embryonic mouse heart during early and late organogenesis}, volume={112}, ISSN={["0301-5564"]}, DOI={10.1007/s004180050417}, abstractNote={Hexokinase (HK) catalyzes the first step in glucose metabolism, that is, the conversion of glucose to glucose-6-phosphate (G6P). Four HK isoforms have been identified, of which HK-I is predominant in embryonic and fetal tissues. HK-I has been studied in preimplantation embryos and in fetal stages, but little is known about its activity or expression in the early postimplantation embryo. We evaluated HK-I expression, HK-I activity, and glycolytic metabolism in the embryonic mouse heart during early [gestational day (gd) 9.5] and late (gd 13.5) organogenesis. Immunohistochemistry demonstrated that HK-I is localized mainly in the heart at both stages, with stronger expression on gd 13.5. Densitometry after SDS-PAGE/western analysis confirmed higher immunodetectable HK-I protein levels in hearts on gd 13.5 vs gd 9.5. By contrast, RT-PCR demonstrated higher HK-I mRNA expression on gd 9.5 vs gd 13.5. Similarly, cardiac HK-I activity (conversion of glucose to G6P) and glycolysis (conversion of glucose to lactate) were higher on gd 9.5 than on gd 13.5. These results suggest a complex regulation of HK-I expression and activity in the embryonic heart during organogenesis, involving a change in the intrinsic activity of the enzyme with development. HK-I appears to play an important role in glucose metabolism during this critical stage of cardiogenesis.}, number={5}, journal={HISTOCHEMISTRY AND CELL BIOLOGY}, author={Fritz, HL and Smoak, IW and Branch, S}, year={1999}, month={Nov}, pages={359–365} }
 @article{smoak_emanuel_1998, title={Tolbutamide: Placental transfer, tissue distribution, and metabolic effects in murine embryos}, volume={82}, number={4}, journal={Pharmacology and Toxicology}, author={Smoak, I. W. and Emanuel, A. N.}, year={1998}, pages={203–208} }
 @article{smoak_1997, title={Brief hypoglycemia alters morphology, function, and metabolism of the embryonic mouse heart}, volume={11}, number={4}, journal={Reproductive Toxicology (Elmsford, N.Y.)}, author={Smoak, I. W.}, year={1997}, pages={495–502} }
 @article{smoak_amiss_1997, title={Chlorobutanol: maternal serum levels and placental transfer in the mouse}, volume={39}, number={5}, journal={Veterinary and Human Toxicology}, author={Smoak, I. W. and Amiss, T. J.}, year={1997}, pages={287–290} }
 @article{piscopo_smoak_1997, title={Comparison of effects of albendazole sulfoxide on in vitro produced bovine embryos and rat embryos}, volume={58}, number={9}, journal={American Journal of Veterinary Research}, author={Piscopo, S. E. and Smoak, I. W.}, year={1997}, pages={1038–1042} }
 @article{barnes_smoak_1997, title={Immunolocalization and heart levels of GRP94 in the mouse during post-implantation development}, volume={196}, ISSN={["0340-2061"]}, DOI={10.1007/s004290050102}, abstractNote={Glucose-regulated proteins (GRPs), which belong to the highly conserved family of stress proteins, are resident to the endoplasmic reticulum and function as molecular chaperones. Heat shock proteins have been shown to be developmentally regulated, but little work has been done to investigate the expression of GRPs during embryogenesis. Therefore, this study examined the distribution of GRP94 within mouse embryos during the period of organogenesis and characterized levels of GRP94 within the developing heart during organogenesis and late fetal stages. Our results demonstrate that the GRP94 protein is constitutively expressed within mouse embryos during early stages of organogenesis and is localized particularly within the developing heart, neuroepithelium, and surface ectoderm tissues. Positive staining for GRP94 remains within developing heart tissues throughout organogenesis and is found primarily within the atrial and ventricular myocardial cells. Western blot analysis of GRP94 expression demonstrates a significantly higher level of GRP94 in embryonic hearts during early stages of organogenesis than in later stages of organogenesis or the fetal period. These results demonstrate that the stress protein GRP94 is constitutively expressed within specific tissues during post-implantation mouse development and suggest that GRPs may play an important role in the process of myocardial cell differentiation and heart development.}, number={4}, journal={ANATOMY AND EMBRYOLOGY}, author={Barnes, JA and Smoak, IW}, year={1997}, month={Oct}, pages={335–341} }
 @article{smoak_hudson_1996, title={Persistent oropharyngeal membrane in a Hereford calf}, volume={33}, ISSN={["0300-9858"]}, DOI={10.1177/030098589603300110}, abstractNote={Persistent oropharyngeal membrane was found in a 6-day-old Hereford calf. The calf was unable to nurse and had not passed feces since birth. Physical examination revealed a fold of tissue spanning the caudal oral cavity, and a barium study demonstrated that food remained within the oral cavity despite a swallowing reflex. A thickened epiglottis was demonstrated radiographically but was not confirmed on necropsy. Necropsy revealed a complete soft-tissue partition between oral cavity and oropharynx with a central blind-ended diverticulum. Only a few cases of persistent oropharyngeal membrane have been reported previously, all in humans. This is the first report of this defect in domestic animals. Possible similarities to the more common persistent anal membrane are discussed.}, number={1}, journal={VETERINARY PATHOLOGY}, author={Smoak, IW and Hudson, LC}, year={1996}, month={Jan}, pages={80–82} }